Core--Biotechnology

核心--生物科技

• 批准号: 7432443
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 24.75万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7432443
• 关键词: Affinity; Biotechnology; Cells; Clone Cells; Engineering; Equipment; Gene Transfer; Generations; Goals; Human Resources; Hybridomas; Immune Sera; Mass Spectrum Analysis; Monoclonal Antibodies; Post-Translational Protein Processing; Process; Production; Proteins; Proteolytic Processing; Reagent; Recombinant Proteins; Research Personnel; Technical Expertise; Techniques; Technology; Variant; carboxylation; experience; glycosylation; monoclonal antibody production; programs

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment.

该核心是本方案项目的必要组成部分。它提供了高度 经验丰富的技术专长，为项目需要，以满足该计划的目标。它提供了许多重组蛋白的生产和纯化，野生型或修饰，为项目。这需要通过细菌宿主以及真核宿主如CHO-K1和变体细胞进行生产，所述变体细胞已被工程化用于蛋白质的适当翻译后修饰，如适当糖基化、γ羧化、蛋白水解加工、纯度和功能完整性分析。该核心提供了向细胞的基因转移、克隆和这些经修饰细胞的克隆的扩增。它提供针对新蛋白质或蛋白质修饰的特异性抗血清。此外，它在单克隆抗体生产，选择和建立长期高亲和力和高特异性单克隆抗体试剂的项目中非常专业，这些试剂不存在。专门知识与新参与各种技术的调查人员分享。核心人员协助各调查人员进行更多的调查， 复杂的电泳技术和质谱处理。他们监督共用设备的适当使用。