Core--Biotechnology

核心--生物科技

• 批准号: 7432443
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 24.75万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7432443
• 关键词: Affinity; Biotechnology; Cells; Clone Cells; Engineering; Equipment; Gene Transfer; Generations; Goals; Human Resources; Hybridomas; Immune Sera; Mass Spectrum Analysis; Monoclonal Antibodies; Post-Translational Protein Processing; Process; Production; Proteins; Proteolytic Processing; Reagent; Recombinant Proteins; Research Personnel; Technical Expertise; Techniques; Technology; Variant; carboxylation; experience; glycosylation; monoclonal antibody production; programs

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment.

该核心是本计划项目的必要组成部分。它提供了高度 实现该计划目标所需的项目的经验丰富的技术专业知识。它为项目提供了许多野生型或修饰的重组蛋白的生产和纯化。这需要细菌宿主以及真核宿主（例如 CHO-K1）和变异细胞进行生产，这些宿主经过工程改造，可以对蛋白质进行适当的翻译后修饰，例如适当的糖基化、γ 羧化、蛋白水解加工、纯度和功能完整性分析。该核心提供基因转移至细胞克隆以及修饰细胞的这些克隆的扩增。它提供针对新蛋白质或针对蛋白质修饰的特异性抗血清。此外，它在用于单克隆抗体生产、选择和建立长期高亲和力和高特异性单克隆抗体试剂的杂交瘤生成方面非常专业，以用于其他不存在此类试剂的项目。与新涉足各种技术的研究人员分享专业知识。核心人员协助各调查人员开展更多工作 复杂的电泳技术和质谱处理。他们监督共用设备的正确使用。