Core--Biotechnology

核心--生物科技

• 批准号: 7105009
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 21.44万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7105009
• 关键词: biomedical facility; biotechnology; hybridomas; immunologic substance development /preparation; mass spectrometry; molecular cloning; monoclonal antibody; peptide chemical synthesis; technology /technique development; thromboplastin; tissue /cell preparation; tissue resource /registry; transfection; vascular endothelium

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment.

该核心是该程序项目的必要组成部分。它提供了高度的 为满足计划目标所需的项目经验丰富的技术专长。它为项目提供了许多重组蛋白的生产和纯化，用于项目。这需要细菌宿主以及经过精心设计的诸如CHO-K1和变体细胞等真核宿主的生产，这些细胞是针对蛋白质进行适当的翻译后修饰的，例如适当的糖基化，γ羧化，蛋白水解处理，蛋白水解处理，纯度和功能整合的分析，分析。该核心为这些修饰细胞的这些克隆的克隆和扩展提供了基因转移。它为新蛋白质提供了抗血清，或针对蛋白质修饰的特异性。同样，它是杂交瘤生成的非常专家，用于单克隆抗体的产生，选择并建立长期高亲和力和高度特异性的单克隆抗体试剂，用于这些试剂不存在的项目。专业知识与新参与各种技术的调查人员共享。核心人员协助各种调查人员进行更多 精致的电泳技术和质谱的处理。他们监督了普通共享设备的正确使用。