CORE C--ADMINISTRATIVE

核心C--行政

• 批准号: 7432437
• 负责人: THOMAS Scott EDGINGTON
• 金额: $ 23.84万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7432437
• 关键词: Advisory Committees; Affinity; Cell Culture Techniques; Cells; Clone Cells; Data; Engineering; Equipment; Gene Transfer; Generations; Goals; Human Resources; Hybridomas; Immune Sera; Institutes; Mass Spectrum Analysis; Monoclonal Antibodies; Post-Translational Protein Processing; Process; Production; Proteins; Proteolytic Processing; Reagent; Recombinant Proteins; Recruitment Activity; Research Personnel; Resources; Safety Management; Sterility; Technical Expertise; Techniques; Technology; Variant; carboxylation; experience; glycosylation; monoclonal antibody production; programs

This core is a necessary and integral component of this Program Project. It provides highly experienced technical expertise for the projects needed to meet the goals of the Program. It provides for the production and purification of the many recombinant proteins, wild-type or modified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translational modification of proteins such as proper glycosylation, gamma carboxylation, proteolytic processing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highly specific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment. The core personnel are among the most expert personnel in sterile technique, cell culture, monoclonal antibody production, safety management, and recombinant protein production in the Institute.

该核心是本方案项目的必要组成部分。它提供了高度 经验丰富的技术专长，为项目需要，以满足该计划的目标。它 提供了许多重组蛋白的生产和纯化，野生型或 修改，对于项目。这需要通过细菌宿主以及真核宿主如CHO-K1和变体细胞进行生产，所述变体细胞已经被工程化以进行适当的翻译后修饰。 蛋白质的修饰，例如适当的糖基化、γ羧化、蛋白水解 处理、纯度和功能完整性分析。该核心提供了向细胞的基因转移、克隆和这些经修饰细胞的克隆的扩增。它提供了新蛋白质的抗血清或对蛋白质修饰具有特异性的抗血清。此外，它是非常专业的杂交瘤代单克隆抗体的生产，选择，并建立长期的高亲和力和高度 特异性单克隆抗体试剂，用于没有此类试剂的项目。专门知识与新参与各种技术的调查人员分享。核心人员协助各种研究人员进行更复杂的电泳技术和质谱处理。他们监督共用设备的适当使用。核心人员是研究所在无菌技术、细胞培养、单克隆抗体生产、安全管理、重组蛋白生产等方面最专业的人员。