Core--Microchemical

核心--微量化学

• 批准号: 6861193
• 负责人: CHARLOTTE BROWN
• 金额: $ 2.24万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6861193
• 关键词: biomedical facility; chromatography; nucleic acid chemical synthesis; nucleic acid sequence; protein sequence; reagent /indicator

The Microchemical Facility is an integral component of this program. Continuation of this work will be made possible by the use of expertise, equipment and supplies provided by this core. In addition for providing a center for intellectual interactions, experimental design, trouble-shooting and other consultation, the Microchemical Facility core operates as a service core which provides a number of biologic reagents including oligonucleotide primers heavily used in this research. In addition, the core accomplishes automated DNA sequencing of transcripts (RT-PCR) products) and cloned DNA (i.e., plasmid, retrovirus constructs and AAV vectors). The facility will also provide mutation analysis of experimental cell lines and for molecular diagnosis of patients entering into a clinical trial. The high-capacity sequencing capability will also be required to validate vector constructs and, in the Gene Scan mode, for quantitative analysis of gene insertion into target cells. The Microchemical Facility core also provides a host of other instrumentation for analysis and synthesis (e.g. for peptide sequencing, peptide synthesis, etc.) which will be used on a less frequent basis.

微量化学设施是该计划的一个组成部分。将利用该核心提供的专门知识、设备和用品继续开展这项工作。除了提供智力互动，实验设计，故障排除和其他咨询中心外，微量化学设施核心还作为服务核心运营，提供许多生物试剂，包括本研究中大量使用的寡核苷酸引物。此外，核心完成转录物（RT-PCR）产物）和克隆DNA（即，质粒、逆转录病毒构建体和AAV载体）。的 该设施还将提供实验细胞系的突变分析和进入临床试验的患者的分子诊断。还需要高容量测序能力来验证载体构建体，并在基因扫描模式下对插入靶细胞的基因进行定量分析。微量化学设施核心还提供了许多其他分析和合成仪器（例如肽测序、肽合成等）。其将在较不频繁的基础上使用。