Core--Microchemical

微化学设施是该计划的一个组成部分。利用这一核心提供的专门知识、设备和用品将使这项工作得以继续。除了提供智力互动、实验设计、故障排除和其他咨询中心外，微化学设施核心还作为服务核心运作，提供许多生物试剂，包括本研究中大量使用的寡核苷酸引物。此外，该核心完成转录本（RT-PCR）产物)和克隆DNA（即质粒、逆转录病毒构建体和AAV载体）的自动DNA测序。这个

The Microchemical Facility is an integral component of this program. Continuation of this work will be made possible by the use of expertise, equipment and supplies provided by this core. In addition for providing a center for intellectual interactions, experimental design, trouble-shooting and other consultation, the Microchemical Facility core operates as a service core which provides a number of biologic reagents including oligonucleotide primers heavily used in this research. In addition, the core accomplishes automated DNA sequencing of transcripts (RT-PCR) products) and cloned DNA (i.e., plasmid, retrovirus constructs and AAV vectors). The facility will also provide mutation analysis of experimental cell lines and for molecular diagnosis of patients entering into a clinical trial. The high-capacity sequencing capability will also be required to validate vector constructs and, in the Gene Scan mode, for quantitative analysis of gene insertion into target cells. The Microchemical Facility core also provides a host of other instrumentation for analysis and synthesis (e.g. for peptide sequencing, peptide synthesis, etc.) which will be used on a less frequent basis.