Core--Microchemical

微型化学设施是该方案的一个组成部分。通过使用该核心提供的专门知识、设备和用品，这项工作将得以继续。除了为智力互动、实验设计、故障排除和其他咨询提供中心外，微化学设施核心还作为服务核心运作，提供许多生物试剂，包括在这项研究中大量使用的寡核苷酸引物。此外，该核心还完成转录本(RT-PCR)产物和克隆DNA(即，质粒、逆转录病毒载体和AAV载体)的自动DNA测序。这个 该设备还将提供实验细胞系的突变分析，并为进入临床试验的患者进行分子诊断。大容量测序能力也将被需要来验证载体构建，并在基因扫描模式下，用于对插入目标细胞的基因进行定量分析。微化学设备核心还提供大量其他分析和合成仪器(例如，多肽测序、多肽合成等)。它将在较低频率的基础上使用。

The Microchemical Facility is an integral component of this program. Continuation of this work will be made possible by the use of expertise, equipment and supplies provided by this core. In addition for providing a center for intellectual interactions, experimental design, trouble-shooting and other consultation, the Microchemical Facility core operates as a service core which provides a number of biologic reagents including oligonucleotide primers heavily used in this research. In addition, the core accomplishes automated DNA sequencing of transcripts (RT-PCR) products) and cloned DNA (i.e., plasmid, retrovirus constructs and AAV vectors). The facility will also provide mutation analysis of experimental cell lines and for molecular diagnosis of patients entering into a clinical trial. The high-capacity sequencing capability will also be required to validate vector constructs and, in the Gene Scan mode, for quantitative analysis of gene insertion into target cells. The Microchemical Facility core also provides a host of other instrumentation for analysis and synthesis (e.g. for peptide sequencing, peptide synthesis, etc.) which will be used on a less frequent basis.