Genetic Analysis of Conotoxin Targets

芋螺毒素靶标的遗传分析

基本信息

  • 批准号:
    6931200
  • 负责人:
  • 金额:
    $ 24.95万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-08-02 至 2009-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The goal of the proposed research is to provide a genetic-based pharmacology for the study of synaptic and neural function. The tremendous diversity of toxins from the venoms of predatory Conus snail species provide a natural combinatorial-based pharmacology that can be used to selectively bind to the members of the large and diverse group of neurotransmitter receptors that mediate synaptic communication. We propose to use C. elegans to rapidly purify new toxins that will be of interest to the broad community of neurobiologists. We have three major aims. The first aim is to identify and purify peptide toxins from the venoms of Conus snails that disrupt the behavior of the soil nematode C. elegans by perturbing nervous system function. A long-term goal of this strategy is to use Conus toxins as specific probes that will permit the identification of new gene products that contribute to nervous system function. The second aim is to identify and purify peptide toxins that block specific ligand-gated currents in muscles and neurons of C. elegans. These toxins will be invaluable for C. elegans neurobiologists and will allow for detailed mechanistic studies of synaptic transmission in C. elegans that currently are not possible because of the lack of specific pharmacological agents to acutely block specific classes of currents. We will use genetic and electrophysiological strategies to determine the site and mechanism of action of the purified Conus toxins. We have also used tissue-specific promoters to express active Conus toxins in transgenic worms. The Conus Im1 toxin blocks approximately 50% of the ACh-gated current at the neuromuscular junction. The gene products that contribute to this Im1-sensitive current have not yet been identified. Using a new genetic strategy, we will identify gene products that are required for this portion of the synaptic cholinergic current. The tremendous diversity inherent in Conus peptides, the specific and potent receptor interactions, and the fact that these peptides can be expressed in transgenic organisms in a tissue specific manner, may in the future provide a framework for designing genetic-based therapies for neurological disorders.
描述(由申请人提供):拟议研究的目标是为突触和神经功能的研究提供基于遗传的药理学。来自肉食性芋螺属蜗牛物种毒液的毒素的巨大多样性提供了一种天然的基于组合的药理学,其可用于选择性地结合介导突触通信的大而多样的神经递质受体组的成员。我们建议使用C。这种新的毒素将引起广大神经生物学家的兴趣。我们有三个主要目标。第一个目的是鉴定和纯化芋螺毒液中干扰土壤线虫C. elegans通过扰乱神经系统功能。这一策略的长期目标是使用芋螺毒素作为特异性探针,这将允许识别有助于神经系统功能的新基因产物。第二个目标是鉴定和纯化阻断C.优雅的这些毒素对C. elegans神经生物学家,并将允许在C.目前还不可能,因为缺乏特定的药理学试剂来急性阻断特定类别的电流。我们将使用遗传学和电生理学策略来确定纯化的芋螺毒素的作用部位和机制。我们还使用组织特异性启动子在转基因蠕虫中表达活性芋螺毒素。芋螺Im1毒素阻断神经肌肉接头处大约50%的乙酰胆碱门控电流。目前还没有确定的基因产物,有助于这Im1敏感电流。使用一种新的遗传策略,我们将确定这部分突触胆碱能电流所需的基因产物。芋螺肽固有的巨大多样性,特异性和有效的受体相互作用,以及这些肽可以以组织特异性方式在转基因生物中表达的事实,可能在未来为设计神经系统疾病的遗传疗法提供框架。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Andres Villu Maricq其他文献

Andres Villu Maricq的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Andres Villu Maricq', 18)}}的其他基金

Peptidergic Modulation of NMDA-Receptor Mediated Neurotransmission
NMDA 受体介导的神经传递的肽能调节
  • 批准号:
    10280822
  • 财政年份:
    2021
  • 资助金额:
    $ 24.95万
  • 项目类别:
Regulation of NMDAR-Mediated Synaptic Signaling
NMDAR 介导的突触信号传导的调节
  • 批准号:
    10533340
  • 财政年份:
    2021
  • 资助金额:
    $ 24.95万
  • 项目类别:
Peptidergic Modulation of NMDA-Receptor Mediated Neurotransmission
NMDA 受体介导的神经传递的肽能调节
  • 批准号:
    10622524
  • 财政年份:
    2021
  • 资助金额:
    $ 24.95万
  • 项目类别:
Regulation of NMDAR-Mediated Synaptic Signaling
NMDAR 介导的突触信号传导的调节
  • 批准号:
    10346564
  • 财政年份:
    2021
  • 资助金额:
    $ 24.95万
  • 项目类别:
Peptidergic Modulation of NMDA-Receptor Mediated Neurotransmission
NMDA 受体介导的神经传递的肽能调节
  • 批准号:
    10443850
  • 财政年份:
    2021
  • 资助金额:
    $ 24.95万
  • 项目类别:
Mechanistic studies of synaptopathies associated with Alzheimer's risk factors
与阿尔茨海默病危险因素相关的突触病的机制研究
  • 批准号:
    9980770
  • 财政年份:
    2019
  • 资助金额:
    $ 24.95万
  • 项目类别:
Mechanistic studies of synaptopathies associated with Alzheimer's risk factors
与阿尔茨海默病危险因素相关的突触病的机制研究
  • 批准号:
    9808919
  • 财政年份:
    2019
  • 资助金额:
    $ 24.95万
  • 项目类别:
2015 Modulation of Neural Circuits & Behavior Gordon Research Conference
2015 神经回路的调制
  • 批准号:
    8909848
  • 财政年份:
    2015
  • 资助金额:
    $ 24.95万
  • 项目类别:
Glutamate-Mediated Neurotransmission and the Control of Behavior
谷氨酸介导的神经传递和行为控制
  • 批准号:
    9009657
  • 财政年份:
    2015
  • 资助金额:
    $ 24.95万
  • 项目类别:
Glutamate-Mediated Neurotransmission and the Control of Behavior
谷氨酸介导的神经传递和行为控制
  • 批准号:
    9128053
  • 财政年份:
    2015
  • 资助金额:
    $ 24.95万
  • 项目类别:

相似海外基金

The rise of heterobranchs (Gastropoda) – comparative microanatomy and early evolution
异分支(腹足纲)的兴起——比较显微解剖学和早期进化
  • 批准号:
    419974396
  • 财政年份:
    2019
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Research Grants
Failure in a field of success - phylogeny, classification and palaeodiversity of Slit Shells (order Pleurotomariida: Gastropoda)
成功领域的失败——裂壳的系统发育、分类和古多样性(侧腹目:腹足纲)
  • 批准号:
    413609851
  • 财政年份:
    2018
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Research Grants
Combining target DNA enrichment and morphology to understand the evolution of Dexiarchia (Nudibranchia, Heterobranchia, Gastropoda, Mollusca)
结合目标 DNA 富集和形态学来了解 Dexiarchia(裸鳃亚纲、异鳃​​纲、腹足纲、软体动物)的进化
  • 批准号:
    384170056
  • 财政年份:
    2017
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Research Grants
Understanding kleptoplasty in Sacoglossa (Heterobranchia, Gastropoda) - a cellular histochemical and genomic approach
了解 Sacoglossa(异鳃纲、腹足纲)的盗贼成形术 - 一种细胞组织化学和基因组方法
  • 批准号:
    280302282
  • 财政年份:
    2015
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Research Grants
REVSYS: Systematic revision of onchidiid slugs (Mollusca, Gastropoda, Pulmonata)
REVSYS:兰花蛞蝓(软体动物、腹足动物、肺动物)的系统修订
  • 批准号:
    1419394
  • 财政年份:
    2013
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Standard Grant
DISSERTATION RESEARCH: Remnants of a Diverse Past: Assessing the Phylogenetic position of recently extinct Pleurocerid snails (Gastropoda: Pleuroceridae)
论文研究:多样化过去的遗迹:评估最近灭绝的侧蜗牛(腹足纲:侧蜗牛科)的系统发育位置
  • 批准号:
    1110638
  • 财政年份:
    2011
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Standard Grant
REVSYS: Systematic revision of onchidiid slugs (Mollusca, Gastropoda, Pulmonata)
REVSYS:兰花蛞蝓(软体动物、腹足动物、肺动物)的系统修订
  • 批准号:
    1050631
  • 财政年份:
    2011
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Standard Grant
PEET: Monographic revision of taxa of marine pulmonates (Mollusca, Gastropoda)
PEET:海洋肺动物类群(软体动物、腹足纲)专题修订
  • 批准号:
    0933276
  • 财政年份:
    2009
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Standard Grant
Evolution, Phylogenie und Taxonomie ausgewählter Überfamilien der Gastropoda im Zeitraum obere Trias bis mittlerer Jura
上三叠世至中侏罗世腹足纲选定总科的进化、系统发育和分类
  • 批准号:
    109935771
  • 财政年份:
    2009
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Research Grants
Collaborative Research: REVSYS: Worm-snails revised (Mollusca: Gastropoda)
合作研究:REVSYS:蠕虫-蜗牛修订版(软体动物:腹足纲)
  • 批准号:
    0841777
  • 财政年份:
    2009
  • 资助金额:
    $ 24.95万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了