Developing proteomics to look at the regulation of innate lymphoid cells by IL-33.

开发蛋白质组学来研究 IL-33 对先天淋巴细胞的调节。

• 批准号: 2593375
• 金额: --
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2593375
• 关键词: Developing; proteomics; look; regulation; innate

Type 2 innate lymphoid cells (ILC2s) play key roles in mediating type 2 immune responses, however beyond their ability to produce cytokines the function and regulation is still poorly understood. ILC2s are activated by IL-33 and IL-25, both of which are important in the response to helminth infections. In this project will look at the changes in ILC2s induced by these cytokines, focusing on functions that would help shape the Th2 immune response. Previous studies on ILC2s have been limited by the low numbers of ILC2s in the body, however recent advances in proteomics and mass spectrometry mean it in now possible use these techniques to look at the signalling and functional changes in these cells. This will be combined with loss of function and small molecule inhibitor studies to dissect the signalling pathways that control the function of these cells, initially focusing on the p38 MAPK pathway.

2 型先天淋巴细胞 (ILC2) 在介导 2 型免疫反应中发挥着关键作用，但除了产生细胞因子的能力之外，其功能和调节仍知之甚少。 ILC2 由 IL-33 和 IL-25 激活，这两种物质对于应对蠕虫感染都很重要。在这个项目中，我们将研究这些细胞因子诱导的 ILC2 的变化，重点关注有助于塑造 Th2 免疫反应的功能。先前对 ILC2 的研究因体内 ILC2 数量较少而受到限制，但是蛋白质组学和质谱学的最新进展意味着现在可以使用这些技术来观察这些细胞中的信号传导和功能变化。这将与功能丧失和小分子抑制剂研究相结合，剖析控制这些细胞功能的信号通路，最初关注 p38 MAPK 通路。