Developing proteomics to look at the regulation of innate lymphoid cells by IL-33.

2型先天性淋巴样细胞（ILC 2）在介导2型免疫应答中起关键作用，然而除了它们产生细胞因子的能力之外，其功能和调节仍然知之甚少。ILC 2被IL-33和IL-25激活，这两种物质在蠕虫感染的应答中都很重要。在这个项目中，我们将研究这些细胞因子诱导的ILC 2的变化，重点关注有助于形成Th 2免疫反应的功能。以前对ILC 2的研究受到体内ILC 2数量较少的限制，但是蛋白质组学和质谱学的最新进展意味着现在可以使用这些技术来观察这些细胞中的信号传导和功能变化。这将与功能丧失和小分子抑制剂研究相结合，以剖析控制这些细胞功能的信号通路，最初集中在p38 MAPK通路上。

Type 2 innate lymphoid cells (ILC2s) play key roles in mediating type 2 immune responses, however beyond their ability to produce cytokines the function and regulation is still poorly understood. ILC2s are activated by IL-33 and IL-25, both of which are important in the response to helminth infections. In this project will look at the changes in ILC2s induced by these cytokines, focusing on functions that would help shape the Th2 immune response. Previous studies on ILC2s have been limited by the low numbers of ILC2s in the body, however recent advances in proteomics and mass spectrometry mean it in now possible use these techniques to look at the signalling and functional changes in these cells. This will be combined with loss of function and small molecule inhibitor studies to dissect the signalling pathways that control the function of these cells, initially focusing on the p38 MAPK pathway.