The Acute Phase Response in Atherosclerosis

动脉粥样硬化的急性期反应

• 批准号: 6915052
• 负责人: STEPHEN V DESIDERIO
• 金额: $ 40.88万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-21 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6915052
• 关键词: acute phase protein; atherosclerosis; gene expression; genetic transcription; immunocytochemistry; immunopathology; immunoregulation; inflammation; interleukin 6; laboratory mouse; microarray technology; pathologic process; transcription factor

DESCRIPTION (provided by applicant): A growing body of evidence supports a prominent role for inflammation in the development of atherosclerosis. The evolution of atheromatous lesions is guided in part by the action of inflammatory mediators, and in apparently healthy subjects, the presence of systemic inflammatory markers is positively correlated with the subsequent occurrence of cardiovascular disease. Nonetheless, the contributions of systemic inflammation to development of atherosclerosis are not well understood. The major component of systemic inflammation, the acute phase response (APR), is triggered in two phases. The first phase leads to the production of proinflammatory cytokines such as IL-lbeta, tumor necrosis factor alpha (TNFalpha) and IL-6 at the site of injury. The second phase involves the action of those cytokines on target organs such as the liver, where synthesis of acute phase proteins is stimulated or suppressed. IL-6, an indicator of increased risk for future myocardial infarction, exerts its effects on APR genes principally through the transcription factor Stat3. Stat3 is expressed as two isoforms with distinct biochemical properties. The use of an alternative splice acceptor site specifies the Stat3beta (Stat 3beta) isoform. We have used a novel approach to generate mice that are selectively unable to synthesize the Stat3beta isoform. Our preliminary observations uncover a role for Stat3beta in the modulation of Stat3-mediated transcription, and suggest that Stat3beta is essential for normal recovery from systemic inflammation. To explore the role of Stat3 isoforms in the regulation of the acute phase response, and the relation of the acute phase response to atherosclerosis, we will pursue three aims. We will develop detailed transcriptional maps of the hepatic and endothelial acute phase responses in normal and Stat3b-deficient mice by genome-wide microarray assays. Using a combination of biochemical and computational approaches, we will define how Stat3beta modulates expression of Stat3-responsive genes. Third, we will test the hypothesis that chronic systemic inflammation promotes development of atherosclerosis by examining the influence of Stat3beta-deficiency on development of atherosclerosis in mouse models.

描述（由申请人提供）：越来越多的证据支持炎症在动脉粥样硬化发展中的重要作用。动脉粥样硬化病变的进展部分由炎症介质的作用引导，并且在表面健康的受试者中，全身炎症标记物的存在与随后的心血管疾病的发生呈正相关。尽管如此，全身炎症对动脉粥样硬化发展的作用还不清楚。全身性炎症的主要组成部分，急性期反应（APR），在两个阶段触发。第一阶段导致在损伤部位产生促炎细胞因子，例如IL-1 β、肿瘤坏死因子α（TNF α）和IL-6。第二阶段涉及这些细胞因子对靶器官如肝脏的作用，其中刺激或抑制急性期蛋白的合成。IL-6是未来心肌梗死风险增加的指标，主要通过转录因子Stat 3对APR基因发挥作用。Stat 3表达为具有不同生化特性的两种同种型。选择性剪接受体位点的使用指定了Stat 3beta（Stat 3beta）同种型。我们使用了一种新的方法来产生选择性无法合成Stat 3 β亚型的小鼠。我们的初步观察揭示了Stat 3 β在Stat 3介导的转录调节中的作用，并表明Stat 3 β对全身性炎症的正常恢复至关重要。为了探索Stat 3亚型在急性期反应调节中的作用，以及急性期反应与动脉粥样硬化的关系，我们将追求三个目标。我们将通过全基因组微阵列分析开发正常和Stat 3b缺陷小鼠肝脏和内皮细胞急性期反应的详细转录图谱。使用生物化学和计算方法相结合，我们将定义Stat 3beta如何调节Stat 3响应基因的表达。第三，我们将通过检测Stat 3 β缺乏对小鼠模型动脉粥样硬化发展的影响来检验慢性全身性炎症促进动脉粥样硬化发展的假设。