Dark-Field Hard-X-ray Microscope

暗场硬 X 射线显微镜

• 批准号: 6884925
• 负责人: Melvin Arthur Piestrup
• 金额: $ 10万
• 依托单位: ADELPHI TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884925
• 关键词: X ray microscopy; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; contrast media; gold; image enhancement; molecular /cellular imaging; optics; phantom model; radiation dosage; tomography

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase 1 project will develop a hard-x-ray microscope capable of producing dark-field images for the detection of colloidal gold particles. Gold colloidal particles are used as labels in the study of antigens and cell and protein structure. In prior research, Adelphi Technology Inc. has developed compound refractive lenses for use in an x-ray microscope that has been used to image biological material in the bright-field mode. A prototype dark-field microscope will be developed by modifying this existing microscope. The potential for successful development of the prototype imaging system is very high because prior work by other researchers using synchrotron sources that have demonstrated the ability to detect gold particle sizes down to 20 nm in very thin specimens. The use of harder x-rays promises detection of smaller size particles inside thicker specimens and the possibility of the use of tomography for volume analysis. Replacing the synchrotron with a conventional x-ray tube as the x-ray source permits commercial and scientific use outside national laboratories. Issues to be explored in Phase I include: adequacy of x-ray source intensity, dose to specimen, expected contrast, and resolution.

描述（由申请人提供）：这个小企业创新研究第1阶段项目将开发一种硬X射线显微镜，能够产生用于检测胶体金颗粒的暗场图像。胶体金颗粒在抗原、细胞和蛋白质结构的研究中用作标记。在之前的研究中，Adelphi Technology Inc.已经开发了用于X射线显微镜的复合折射透镜，该X射线显微镜已经用于以明场模式对生物材料成像。一个原型暗场显微镜将通过修改现有的显微镜。成功开发原型成像系统的潜力非常高，因为其他研究人员使用同步加速器光源的先前工作已经证明了在非常薄的标本中检测到20 nm以下金颗粒尺寸的能力。使用较硬的X射线有望检测较厚样品内的较小尺寸的颗粒，并有可能使用断层扫描进行体积分析。用传统的X射线管代替同步加速器作为X射线源，可以在国家实验室之外进行商业和科学用途。在第一阶段要探讨的问题包括：足够的X射线源强度，剂量标本，预期的对比度和分辨率。