Quantitave Analysis and Mapping of Metals in Tissues

组织中金属的定量分析和绘图

• 批准号: 6991076
• 负责人: ZEWU CHEN
• 金额: $ 9.99万
• 依托单位: X-RAY OPTICAL SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6991076
• 关键词: X ray; biomedical equipment; biomedical equipment development; evaluation /testing; fluorescence; metal poisoning; metals; optics

DESCRIPTION (provided by applicant): Bioessential metals operate at a delicate interface in biological systems between their essential functions and the destructive havoc they cause when they escape their normal control mechanisms. This is especially true for the so-called redox metals (Iron, Copper, Manganese, and Zinc) which are required for development and operation of the central nervous system and cardiovascular system but also play a central role in disorders such as Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), Friedreich's ataxia, and others. In addition, neurotoxic metals such as Lead, Mercury, Arsenic, and Cadmium have been shown to impair neural function even at trace levels. Current methods to monitor the concentration, distribution, and function of metals have specialized benefits but none allow quantitative measurement of the concentration and distribution of the broad range of metals and concentrations of interest. New and efficient x-ray optics now enable monochromatic micro x-ray fluorescence (MMXRF) tools for measurement of virtually all metals in tissue samples with high sensitivity, dynamic range and spatial resolution. Furthermore, this can be achieved with a safe, compact, reliable, low power, cost effective, easy-to- use analyzer that can operate in medical research laboratories directly on tissue samples before they are dried, stained or treated for other complementary studies. The aim of this project is to develop and apply such a point-of-use analyzer. Alzheimer's, Parkinson's, Huntington's, Friedreich's ataxia and other neurological and cardiovascular disorders have been associated with an excess or deficiency of both essential metals and toxic metals in brain and heart tissue. Laboratory based high-sensitivity, quantitative measurement of the concentration and distribution of metal clusters in tissue is now possible by use of new x-ray optics developed by X-ray Optical Systems.

描述（由申请人提供）：生物必需金属在其基本功能和它们在逃避正常控制机制时造成的破坏性破坏之间的生物系统中的微妙界面上起作用。这对于所谓的氧化还原金属（铁、铜、锰和锌）尤其如此，所述氧化还原金属是中枢神经系统和心血管系统的发育和操作所必需的，而且在诸如阿尔茨海默病（AD）、帕金森病（PD）、亨廷顿病（HD）、弗里德赖希共济失调等病症中也起中心作用。此外，神经毒性金属，如铅，汞，砷和镉已被证明损害神经功能，即使在痕量水平。目前监测金属的浓度、分布和功能的方法具有专门的益处，但没有一种方法能够定量测量广泛范围的金属的浓度和分布以及感兴趣的浓度。新型高效的X射线光学器件使单色显微X射线荧光（MMXRF）工具能够以高灵敏度、动态范围和空间分辨率测量组织样品中的几乎所有金属。此外，这可以通过安全、紧凑、可靠、低功耗、成本有效、易于使用的分析仪来实现，该分析仪可以在医学研究实验室中直接对组织样本进行操作，然后对组织样本进行干燥、染色或处理以用于其他补充研究。该项目的目的是开发和应用这样的使用点分析仪。阿尔茨海默氏症、帕金森氏症、亨廷顿氏症、弗里德赖希氏症共济失调以及其他神经系统和心血管疾病都与大脑和心脏组织中必需金属和有毒金属过量或缺乏有关。基于实验室的高灵敏度，定量测量组织中金属簇的浓度和分布，现在可以通过使用新的X射线光学系统开发的X射线光学。