GENE KNOCKOUT/TRANSGENIC ANIMAL SHARED RESOURCE

描述：(申请人描述)基因敲除/转基因动物 共享资源将为西奈山学院的癌症研究人员提供 能够获得最先进的动物生产技术的医药 活体研究的模型。共享资源将提供四个基本要素 为癌症研究提供服务。前两个，转基因或基因的生产 基因敲除小鼠，将使研究人员能够制备新的小鼠品系，这些品系可以 用于研究各种基因产物在癌症生物学中的作用。这个 第三项服务是小鼠品系的冷冻保存，这将使 研究人员将创建一个重要的小鼠品系知识库，而不需要 无限期繁育这些品系的必要性。最后的服务，重新派生 这将使西奈山的癌症研究人员能够获得老鼠 来自其他机构的调查人员的线路，最终可以使用 在西奈山的啮齿动物特有的无病原体屏障设施内。这 是一项重要的能力，因为其他机构的动物可能是 感染各种病原体的小鼠不能直接进入 西奈山的屏障设施。通过以下方式提供这些服务 共享资源将增强研究项目的类型，可以 由西奈山的癌症研究人员进行。

DESCRIPTION: (Applicant's Description) The Gene Knockout/Transgenic Animal Shared Resource will provide cancer researchers at the Mount Sinai School of Medicine with access to state-of-the-art technology for producing animal models for in vivo studies. The Shared Resource will provide four essential services for cancer research. The first two, production of transgenic or gene knockout mice, will allow researchers to prepare novel mouse lines that can be used to study the effects of various gene products in cancer biology. The third service, cryopreservation of mouse lines, will make it possible for researchers to create a repository of important mouse lines without the necessity to breed these lines indefinitely. The final service, rederivation of mouse lines, will allow cancer researchers at Mount Sinai to obtain mouse lines from investigators at other institutions which can ultimately be used within the rodent specific pathogen-free barrier facility at Mount Sinai. This is an important capability, since animals at other institutions which may be infected with various mouse pathogens can not be directly introduced into the barrier facility at Mount Sinai. The availability of these services through the Shared Resource will enhance the types of research projects that can be performed by cancer researchers at Mount Sinai.