GENE KNOCKOUT/TRANSGENIC ANIMAL SHARED RESOURCE

描述：（申请人的描述）基因敲除/转基因动物 共享资源将提供癌症研究人员在西奈山学校 拥有最先进的动物生产技术的药物 用于体内研究的模型。共享资源将提供四个基本的 为癌症研究提供服务。前两种，生产转基因或基因 敲除小鼠，将使研究人员能够制备新的小鼠品系， 用于研究癌症生物学中各种基因产物的作用。的 第三项服务，小鼠品系的冷冻保存，将使其成为可能， 研究人员创建了一个重要的鼠标线库， 需要无限期地繁殖这些品系。最后的服务，再衍生 小鼠品系，将允许西奈山的癌症研究人员获得小鼠 来自其他机构的研究人员的线路， 在西奈山的啮齿动物专用无病原体屏障设施内。这 是一种重要的能力，因为其他机构的动物可能 感染了各种小鼠病原体不能直接进入 西奈山的屏障设施通过以下方式提供这些服务 共享资源将加强研究项目的类型， 是由西奈山的癌症研究人员完成的

DESCRIPTION: (Applicant's Description) The Gene Knockout/Transgenic Animal Shared Resource will provide cancer researchers at the Mount Sinai School of Medicine with access to state-of-the-art technology for producing animal models for in vivo studies. The Shared Resource will provide four essential services for cancer research. The first two, production of transgenic or gene knockout mice, will allow researchers to prepare novel mouse lines that can be used to study the effects of various gene products in cancer biology. The third service, cryopreservation of mouse lines, will make it possible for researchers to create a repository of important mouse lines without the necessity to breed these lines indefinitely. The final service, rederivation of mouse lines, will allow cancer researchers at Mount Sinai to obtain mouse lines from investigators at other institutions which can ultimately be used within the rodent specific pathogen-free barrier facility at Mount Sinai. This is an important capability, since animals at other institutions which may be infected with various mouse pathogens can not be directly introduced into the barrier facility at Mount Sinai. The availability of these services through the Shared Resource will enhance the types of research projects that can be performed by cancer researchers at Mount Sinai.