GENE KNOCKOUT/TRANSGENIC ANIMAL SHARED RESOURCE

描述：（申请人的描述）基因敲除/转基因动物 共享资源将为西奈山医学院的癌症研究人员提供 拥有最先进的动物生产技术的医学 体内研究模型。共享资源将提供四个基本要素 癌症研究服务。前二、转基因或基因的产生 基因敲除小鼠，将使研究人员能够制备新的小鼠品系 用于研究各种基因产物在癌症生物学中的影响。的 第三项服务，小鼠细胞系的冷冻保存，将使 研究人员创建了一个重要小鼠品系的存储库，而无需 必须无限期地培育这些品系。最终服务，重新衍生 小鼠系，将使西奈山的癌症研究人员获得小鼠 最终可以使用的其他机构调查人员的线路 在西奈山的啮齿动物特异性无病原体屏障设施内。这个 是一项重要的能力，因为其他机构中的动物可能 感染多种小鼠病原体不能直接引入 西奈山的屏障设施。这些服务的可用性通过 共享资源将增强可进行的研究项目的类型 由西奈山的癌症研究人员进行。

DESCRIPTION: (Applicant's Description) The Gene Knockout/Transgenic Animal Shared Resource will provide cancer researchers at the Mount Sinai School of Medicine with access to state-of-the-art technology for producing animal models for in vivo studies. The Shared Resource will provide four essential services for cancer research. The first two, production of transgenic or gene knockout mice, will allow researchers to prepare novel mouse lines that can be used to study the effects of various gene products in cancer biology. The third service, cryopreservation of mouse lines, will make it possible for researchers to create a repository of important mouse lines without the necessity to breed these lines indefinitely. The final service, rederivation of mouse lines, will allow cancer researchers at Mount Sinai to obtain mouse lines from investigators at other institutions which can ultimately be used within the rodent specific pathogen-free barrier facility at Mount Sinai. This is an important capability, since animals at other institutions which may be infected with various mouse pathogens can not be directly introduced into the barrier facility at Mount Sinai. The availability of these services through the Shared Resource will enhance the types of research projects that can be performed by cancer researchers at Mount Sinai.