Mouse Genetics

项目摘要/摘要 Tisch癌症研究所(TCI)小鼠遗传学共享资源基金(MGSRF)为TCI成员提供 利用最先进的设备在小鼠身上生产转基因和基因敲除癌症模型，如 以及相关的啮齿动物胚胎学技术。MGSRF通过注射DNA产生转基因小鼠 小鼠原核阶段的单细胞胚胎，并协助实验室生产 通过使用小鼠胚胎干细胞或CRISPR/Cas9基因编辑来靶向突变小鼠品系。 除了创造新的小鼠品系外，MGSRF还协助TCI实验室维护 通过冷冻保存精子和从其他机构引进新的品系 从冷冻胚胎或精子、活胚胎中恢复小鼠，或通过使用体外受精重新衍生 被运送到ISMMS的个体雄性。该共享资源设施具有悠久的历史 为TCI实验室创造小鼠，MGSRF在癌症项目中的作用将继续加强和 提高TCI成员使用新的突变小鼠模型进行前沿癌症研究的能力。

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Mouse Genetics Shared Resource Facility (MGSRF) provides TCI members with access to state-of-the-art facilities for the production of transgenic and knockout cancer models in mice, as well as related rodent embryology techniques. The MGSRF produces transgenic mice by injection of DNA fragments into pronuclear stage single-cell mouse embryos, and assists laboratories with the production of targeted mutant lines of mice through the use of mouse embryonic stem (ES) cells or CRISPR/Cas9 gene editing. In addition to the creation of novel mouse lines, the MGSRF assists TCI laboratories with the maintenance of existing lines through the cryopreservation of sperm, and with the import of novel lines from other institutions by recovering mice from cryopreserved embryos or sperm, live embryos, or through the use of IVF rederivation of individual males that are shipped to ISMMS. This Shared Resource Facility has a long-standing history of creating mice for TCI laboratories, and the MGSRF’s role in the cancer programs will continue to strengthen and enhance the ability of TCI members to conduct cutting-edge cancer research using novel mutant mouse models.