Mouse Genetics

小鼠遗传学

• 批准号: 10674500
• 负责人: KEVIN A KELLEY
• 金额: $ 12.42万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674500
• 关键词: CRISPR/Cas technology; Cancer Center Support Grant; Cancer Model; Cells; Clone Cells; Core Facility; Cryopreservation; DNA; Dedications; Derivation procedure; Doctor of Philosophy; Education; Embryo; Embryology; Gene Targeting; Generations; Genes; Germ Cells; Individual; Injections; Institution; Knock-out; Laboratories; Lead; Maintenance; Malignant Neoplasms; Methods; Modernization; Mus; Price; Production; Recording of previous events; Research Personnel; Resource Sharing; Resources; Rodent; Role; System; Techniques; Transgenic Mice; Transgenic Organisms; anticancer research; embryo cryopreservation; embryonic stem cell; genome editing; in vivo; male; medical schools; member; mouse genetics; mouse model; mutant; mutant mouse model; novel; programs; sperm cell; sperm cryopreservation; zygote

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Mouse Genetics Shared Resource Facility (MGSRF) provides TCI members with access to state-of-the-art facilities for the production of transgenic and knockout cancer models in mice, as well as related rodent embryology techniques. The MGSRF produces transgenic mice by injection of DNA fragments into pronuclear stage single-cell mouse embryos, and assists laboratories with the production of targeted mutant lines of mice through the use of mouse embryonic stem (ES) cells or CRISPR/Cas9 gene editing. In addition to the creation of novel mouse lines, the MGSRF assists TCI laboratories with the maintenance of existing lines through the cryopreservation of sperm, and with the import of novel lines from other institutions by recovering mice from cryopreserved embryos or sperm, live embryos, or through the use of IVF rederivation of individual males that are shipped to ISMMS. This Shared Resource Facility has a long-standing history of creating mice for TCI laboratories, and the MGSRF’s role in the cancer programs will continue to strengthen and enhance the ability of TCI members to conduct cutting-edge cancer research using novel mutant mouse models.

项目概要/摘要 蒂施癌症研究所 (TCI) 小鼠遗传学共享资源设施 (MGSRF) 为 TCI 成员提供服务 拥有最先进的设施，用于在小鼠中生产转基因和基因敲除癌症模型，例如 以及相关的啮齿动物胚胎学技术。 MGSRF 通过注射 DNA 生产转基因小鼠 片段化为原核阶段单细胞小鼠胚胎，并协助实验室生产 通过使用小鼠胚胎干 (ES) 细胞或 CRISPR/Cas9 基因编辑来靶向小鼠突变系。 除了创建新的小鼠品系外，MGSRF 还协助 TCI 实验室维护 通过精子冷冻保存现有的细胞系，并通过从其他机构进口新的细胞系 从冷冻胚胎或精子、活胚胎中恢复小鼠，或通过体外受精再衍生 被运送到 ISMMS 的个体男性。这个共享资源设施有着悠久的历史 为 TCI 实验室培育小鼠，MGSRF 在癌症项目中的作用将继续加强和 增强 TCI 成员使用新型突变小鼠模型进行尖端癌症研究的能力。