CORE--SPECTROSCOPY SUPPORT

核心——光谱支持

• 批准号: 7142501
• 负责人: Robert C Young
• 金额: $ 11.5万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142501
• 关键词: bioimaging /biomedical imaging; biomedical facility; circular dichroism; fluorescence spectrometry; magnetic resonance imaging; microscopy; neoplasm /cancer; nuclear magnetic resonance spectroscopy; spectrometry

The services offered by the Spectroscopy Support Facility (SSF) cover two distinct areas: 1) applications of nuclear magnetic resonance (NMR), circular dichroism, and fluorescence spectroscopy for studies of biomolecular structure and function, and 2) magnetic resonance microscopy (MRM) for In vivo imaging of small animals especially relevant to cancer research. The Facility houses two well equipped NMR instruments, a 600 MHz (14 Tesla) magnet with a Bruker DMX-600 console, a wide-bore 300 MHz (7 Tesla) magnet with a Bruker DRX-300 console, including a Biospec microimaging accessory. Facility personnel maintain and operate the spectrometers, perform NMR analytical and small-animal imaging services, and offer expert advice and technical support to users. The 600 MHz instrument is fully equipped for heteronuclear multidimensional NMR studies on the structure, dynamics and interactions of biological macromolecules. The 300 MHz instrument is well suited for multinuclear analysis of small molecules and studies of enzyme function involving isotopically-labeled ligands. Small-animal imaging on the 300 MHz instrument is of interest to investigators studying mouse models of cancer. MRM permits monitoring of tumor development, growth, perfusion, vascularization, and response to treatment. New capabilities for optical imaging are being implemented in order to study tumors expressing luminescent or fluorescent proteins in small-animal models. The Spectroscopy Support Facility was rated "Very Good to Excellent" at the last review. In 2003, the Facility was used by 18 peer-reviewed, funded investigators from 9 different Programs in all three Divisions. This represents an 80% increase over the number of users five years ago and addresses the previous concern that the Facility primarily served a small number of investigators in the Biomolecular Structure and Function Program. The broadened impact of the Facility is largely due to the implementation of small animal imaging, which made it possible to support cancer-related research Programs by eight additional users from all three Divisions. After the MR ilmaging services became fully operational, the usage of the 300 MHz instrument increased by two-fold. Over 99% of the total usage is by peer-reviewed, funded investigators. Data obtained with the help of the Facility have resulted in 27 publications in peer-reviewed journals over the past five years.

光谱学支持设施（SSF）提供的服务涵盖两个不同的领域： 1)核磁共振（NMR）、圆二色性和荧光的应用 用于生物分子结构和功能研究的光谱学，以及2）磁共振 显微镜（MRM）用于小动物的体内成像，特别是与癌症研究相关的。该设施拥有两台设备齐全的核磁共振仪器，一台600 MHz（14特斯拉）磁铁，配有布鲁克DMX-600控制台，一台宽孔300 MHz（7特斯拉）磁铁，配有布鲁克DRX-300控制台，包括Biospec显微成像配件。设施人员维护和操作光谱仪，进行核磁共振分析和小动物成像服务，并为用户提供专家咨询和技术支持。600 MHz仪器配备齐全，可用于对生物大分子的结构、动力学和相互作用进行异相多维NMR研究。300 MHz仪器非常适合小分子的多核分析和涉及同位素标记配体的酶功能研究。300 MHz仪器上的小动物成像对研究小鼠癌症模型的研究人员很感兴趣。MRM允许监测肿瘤的发展、生长、灌注、血管形成和对治疗的反应。光学成像的新功能正在实施，以研究在小动物模型中表达发光或荧光蛋白的肿瘤。光谱学支持设施在上次审查中被评为“非常好到优秀”。2003年，来自所有三个司的9个不同方案的18名经同行审查并得到资助的调查员使用了该机制。这比五年前的用户数量增加了80%，并解决了以前的问题，即该设施主要为生物分子结构和功能计划的少数研究人员提供服务。该基金扩大影响的主要原因是实施了小动物成像，这使得来自所有三个司的另外八个用户能够支持与癌症有关的研究方案。在磁共振成像服务全面投入使用后，300 MHz仪器的使用量增加了两倍。超过99%的总使用量是由同行评审，资助的调查人员。在该基金的帮助下获得的数据 在过去五年中，在同行评审的期刊上发表了27篇论文。