Protein Core

肯塔基大学分子医学中心的蛋白质核心与生物医学 促进重组蛋白和其他重组蛋白的表达、纯化和鉴定的研究人员 大分子。它使可用的设备、仪器和专业知识通常在 单独的研究实验室。核心有发酵器，生物反应器和组织培养设施 根据需要在细菌、酵母、昆虫或哺乳动物细胞中表达重组蛋白。它还坚持认为 细胞裂解设备和广泛的重组蛋白纯化设施。此外，a 核心有一系列用于生物物理表征的分析仪器，包括动态 光散射、分光偏振、荧光、等温滴定量热法、高效液体 层析、分析超速离心法和X射线结晶学。结晶学设施包括 用于自动结晶试验的仪器、内部数据收集设备以及 集团(SER-CAT)在高级光子源运营最先进的数据收集设施 在阿贡国家实验室内。一名核心工作人员维护设备，提供培训， 与研究人员就各自的项目进行合作。经验丰富的用户可以直接访问核心设施， 而新用户首先由核心人员进行培训，并协助进行初步实验。用户拥有更多 困难的项目得到核心人员的额外援助。承接服务项目 有选择地针对缺乏专业知识或人员自行开展工作的用户。核心是 每年由代表50-70个研究小组的研究人员使用，超过4200个人使用 为第二阶段科布雷供资的头四年记录的仪器或设施。外展活动 包括核心网站、核心联合教员的演讲、个人和小组培训课程、a 每月的研究系列讲座，以及参加研究生课程和NSF REU暑期项目。这个 CORE在获得新仪器和引进新技术方面很积极。向自给自足转型 区域设施计划在Cobre第三阶段融资过程中进行。

The Protein Core in the University of Kentucky Center for Molecular Medicine works with biomedical investigators to facilitate the expression, purification, and characterization of recombinant proteins and other macromolecules. It makes available equipment, instrumentation, and expertise not normally be found within individual research laboratories. The Core has fermentors, bioreactors, and tissue culture facilities for expressing recombinant protein in bacteria, yeast, insect, or mammalian cells as required. It also maintains cell cracking equipment and an extensive facility for the purification of recombinant proteins. In addition, a range of analytical instruments for biophysical characterization are available in the Core, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, analytical ultracentrifugation, and X-ray crystallography. Crystallography facilities include instruments for automated crystallization trials, in house data collection equipment, and membership in a group (SER-CAT) operating a state-of-the-art data collection facility at the Advanced Photon Source located within Argonne National Laboratory. A Core staff member maintains the equipment, provides training and, works with researchers on their individual projects. Experienced users have direct access to Core facilities, while new users are first trained by Core personnel and assisted in initial experiments. Users with more difficult projects receive additional assistance from the Core personnel. Service projects are undertaken selectively for users who lack the expertise or personnel to carry out the work on their own. The Core is used by investigators representing 50-70 research groups each year, with over 4200 individual uses of instruments or facilities documented for the first four years of Phase II COBRE funding. Outreach activities include the Core web site, talks given by Core associated faculty, individual and group training sessions, a monthly research talk series, and participation in graduate courses and an NSF REU summer program. The Core is active in obtaining new instrumentation and introducing new techniques. Transition to a selfsustaining regional facility is planned over the course of Phase III COBRE funding.