Acquisition of a Varian 600 MHz High-Resolution NMR

获取 Varian 600 MHz 高分辨率 NMR

• 批准号: 6803723
• 负责人: Phil Crews
• 金额: $ 27.16万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6803723
• 关键词: biomedical equipment purchase; chemical structure; chemical structure function; nuclear magnetic resonance spectroscopy

DESCRIPTION (provided by applicant): The aim of this grant application is to purchase a state-of-the-art 600 MHz NMR system equipped with a shielded magnet. It will greatly expand and modernize the campus Nuclear Magnetic Resonance (NMR) Laboratory housed in the Department of Chemistry and Biochemistry. Currently, the department has two NMR spectrometers - a 500 MHz instrument (NSF/NIH funded, 1995) and a 250 MHz instrument (FIMA funded, 1990). Obtaining high quality NMR data is a daily exercise in the research programs of the seven NIH grantees involved in this application. Their programs are investigating research topics that are of tremendous biomedical significance. The current and future initiatives will play a pivotal role in new discoveries that have many broader implications. We believe that the equipment proposed herein will also be an important tool for enhancing our teaching and research. There will be five major and four minor users of the proposed equipment. Two major users (Millhauser and Rohl) are probing the role of protein signaling and folding, which depend on proposed instrument. Three other major users (Crews, Konopelski, and Lokey) depend on the rapid characterization of complex bioorganic compound mixtures. The research of four other minor users involves: radical reagents (Braslau), new chiral synthesis tools (Singaram), metal ions in metalloenzymes and therapeutics (Mascharak), dynamics of active site iron in lipoxygenases (Holman). We propose to obtain a 600 MHz narrow bore NMR spectrometer and there are three companies manufacturing NMR systems operating at 14.1 T including the Bruker ADVANCE-ICE with an Ultrashield 600 Magnet, the JEOL ECA-600, and the Varian Unity Inova 600.These include (a) Magnet, (b) Console, (c) Pulse filed gradients, (d) Wave Form Generators, (e) Probes (with four probes as follows: (i) 1H{13C/X} PFG Tunable Triple Resonance with capabilities for single irradiation of 13C and X frequencies tunable from 15N to 31P; (ii) 1H{15N-31P} PFG indirect detect, the probe of choice for indirect detection of small and large biomolecules; (iii) 1H-19F/15N-31P 3mm PFG Dual Broadband, a probe useful for single resonance studies of sample limited compounds. (iv) A cryo cooled triple resonance probe for maximum sensitivity will be the 1H{13C/15N} 5mm PFG Triple), and (f) a Protasis Corporation CapNMR micro flow probe. The addition of a state-of-the-art 600 MHz instrument will greatly expand the range of experiments including essential capabilities such as those outlined in the research descriptions presented in the proposal. The instrument and its complement of probes will be essential for maintaining modern research with competitive throughput and the training of our students in a modern research environment.

描述（由申请人提供）：本资助申请的目的是购买一个国家的最先进的600兆赫核磁共振系统配备了屏蔽磁铁。它将大大扩大和现代化的校园核磁共振（NMR）实验室设在化学和生物化学系。目前，该部门有两个NMR光谱仪-一个500 MHz仪器（NSF/NIH资助，1995年）和一个250 MHz仪器（FIMA资助，1990年）。获得高质量的NMR数据是参与本申请的七个NIH受资助者研究计划的日常工作。他们的项目正在调查具有巨大生物医学意义的研究课题。当前和未来的举措将在具有更广泛影响的新发现中发挥关键作用。我们相信，本文建议的设备也将是加强我们的教学和研究的重要工具。 拟议设备将有五个主要用户和四个次要用户。两个主要用户（Millhauser和Rohl）正在探索蛋白质信号传导和折叠的作用，这取决于拟议的仪器。其他三个主要用户（Crews，Konopelski和Lokey）依赖于复杂生物有机化合物混合物的快速表征。其他四个小用户的研究涉及：自由基试剂（Braslau），新的手性合成工具（Singaram），金属酶和治疗中的金属离子（Mascharak），脂氧合酶中活性部位铁的动力学（Holman）。 我们建议获得600 MHz窄孔NMR光谱仪，并且有三家公司制造在14.1 T下操作的NMR系统，包括具有Ultrashield 600磁体的Bruker ADVANCE-ICE、JEOL ECA-600和Varian Unity Inova 600。这些包括（a）磁体，（B）控制台，（c）脉冲场梯度，（d）波形发生器，（e）探针（具有如下四种探针：（i）1H{13 C/X} PFG可调谐三重共振，具有从15 N到31 P可调谐的13 C和X频率的单次照射能力;（ii）1H{15 N-31 P} PFG间接检测，用于间接检测小和大生物分子的探针的选择;（iii）1H-19 F/15 N-31 P 3 mm PFG Dual Broadband，一种用于样品有限化合物的单共振研究的探针。(iv)用于最大灵敏度的低温冷却三重共振探针将是1H{13 C/15 N} 5 mm PFG Triple），以及（f）Protasis Corporation CapNMR微流探针。增加一台最先进的600兆赫仪器将大大扩大实验范围，包括提案中研究说明所概述的基本能力。该仪器及其探头的补充将是必不可少的，以保持具有竞争力的吞吐量和我们的学生在现代研究环境中的培训现代研究。