Acquisition of a Varian 600 MHz High-Resolution NMR

获取 Varian 600 MHz 高分辨率 NMR

• 批准号: 6803723
• 负责人: Phil Crews
• 金额: $ 27.16万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6803723
• 关键词: biomedical equipment purchase; chemical structure; chemical structure function; nuclear magnetic resonance spectroscopy

DESCRIPTION (provided by applicant): The aim of this grant application is to purchase a state-of-the-art 600 MHz NMR system equipped with a shielded magnet. It will greatly expand and modernize the campus Nuclear Magnetic Resonance (NMR) Laboratory housed in the Department of Chemistry and Biochemistry. Currently, the department has two NMR spectrometers - a 500 MHz instrument (NSF/NIH funded, 1995) and a 250 MHz instrument (FIMA funded, 1990). Obtaining high quality NMR data is a daily exercise in the research programs of the seven NIH grantees involved in this application. Their programs are investigating research topics that are of tremendous biomedical significance. The current and future initiatives will play a pivotal role in new discoveries that have many broader implications. We believe that the equipment proposed herein will also be an important tool for enhancing our teaching and research. There will be five major and four minor users of the proposed equipment. Two major users (Millhauser and Rohl) are probing the role of protein signaling and folding, which depend on proposed instrument. Three other major users (Crews, Konopelski, and Lokey) depend on the rapid characterization of complex bioorganic compound mixtures. The research of four other minor users involves: radical reagents (Braslau), new chiral synthesis tools (Singaram), metal ions in metalloenzymes and therapeutics (Mascharak), dynamics of active site iron in lipoxygenases (Holman). We propose to obtain a 600 MHz narrow bore NMR spectrometer and there are three companies manufacturing NMR systems operating at 14.1 T including the Bruker ADVANCE-ICE with an Ultrashield 600 Magnet, the JEOL ECA-600, and the Varian Unity Inova 600.These include (a) Magnet, (b) Console, (c) Pulse filed gradients, (d) Wave Form Generators, (e) Probes (with four probes as follows: (i) 1H{13C/X} PFG Tunable Triple Resonance with capabilities for single irradiation of 13C and X frequencies tunable from 15N to 31P; (ii) 1H{15N-31P} PFG indirect detect, the probe of choice for indirect detection of small and large biomolecules; (iii) 1H-19F/15N-31P 3mm PFG Dual Broadband, a probe useful for single resonance studies of sample limited compounds. (iv) A cryo cooled triple resonance probe for maximum sensitivity will be the 1H{13C/15N} 5mm PFG Triple), and (f) a Protasis Corporation CapNMR micro flow probe. The addition of a state-of-the-art 600 MHz instrument will greatly expand the range of experiments including essential capabilities such as those outlined in the research descriptions presented in the proposal. The instrument and its complement of probes will be essential for maintaining modern research with competitive throughput and the training of our students in a modern research environment.

描述(由申请人提供)：本次拨款申请的目的是购买配备屏蔽磁铁的最先进的600 MHz核磁共振系统。它将大大扩展和现代化校园核磁共振(核磁共振)实验室设在化学和生物化学系。目前，该部门有两台核磁共振光谱仪--一台500 MHz仪器(NSF/NIH资助，1995)和一台250 MHz仪器(FIMA资助，1990)。获取高质量的核磁共振数据是参与这一应用的七个NIH受赠者的研究计划中的日常工作。他们的项目正在调查具有巨大生物医学意义的研究课题。当前和未来的倡议将在具有更广泛影响的新发现中发挥关键作用。我们相信，这里提出的设备也将是加强我们的教学和研究的重要工具。 拟议设备将有五个主要用户和四个次要用户。两个主要用户(Millhauser和Rohl)正在探索蛋白质信号和折叠的作用，这取决于所建议的仪器。其他三个主要用户(CROWS、Konopelski和Lokey)依赖于复杂生物有机化合物混合物的快速表征。其他四个次要用户的研究涉及：自由基试剂(Braslau)、新的手性合成工具(Singaram)、金属酶和治疗学中的金属离子(Mascharak)、脂氧合酶中活性部位铁的动力学(Holman)。 我们建议获得一台600 MHz窄口径核磁共振仪，有三家公司生产在14.1T下运行的核磁共振系统，包括带有超声波屏蔽600磁铁的Bruker Advance-ICE，JEOL ECA-600和Varian Unity Inova 600。这些系统包括(A)磁铁，(B)控制台，(C)脉冲场梯度，(D)波形发生器，(E)探头(具有如下四个探头：(I)1H{13C/X}PFG可调三重共振，单次照射13C和X频率的能力从15N到31P可调)；(Ii)1H{15N-31P}PFG间接检测，这是间接检测小分子和大生物分子的首选探针；(Iii)1H-19F/15N-31P 3 mm PFG双宽带，一种适用于有限样品的单共振研究的探针。(Iv)用于获得最高灵敏度的冷冻冷却三重共振探头将是1H{13C/15N}5 mm PFG Triple)，以及(F)Protasis Corporation CapNMR微流动探头。增加一台最先进的600兆赫仪器将大大扩大实验的范围，包括提案中提出的研究说明中概述的基本能力。该仪器及其探头的补充对于保持具有竞争力的现代研究和我们的学生在现代研究环境中的培训至关重要。