Fluorescence microscopy image acquisition/analysis sys

荧光显微镜图像采集/分析系统

• 批准号: 6736158
• 负责人: DAVID G DRUBIN
• 金额: $ 24.56万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6736158
• 关键词: biomedical equipment purchase; fluorescence microscopy; image processing

DESCRIPTION (provided by applicant): State-of-the-art imaging of whole cells and of isolated cytoskeletal elements and organelles is vital to achieving the Specific Aims of the NIH grants awarded to each of the six U.C. Berkeley cell biologists participating in this Shared Instrumentation Grant proposal. Acquisition and analysis of fluorescence images using the latest technologies has become essential to the continued success of each of these research programs. The overall aim of this proposal is to secure funds to acquire a state-of-the-art Olympus imaging system with capabilities that are currently unavailable to the applicants, or that are in such high demand that progress is being significantly hindered by competition for time on the one existing shared system. The capabilities to be provided by this imaging system include Total Internal Reflection (TIRF) microscopy, high-speed multi-color image acquisition for real time analysis of dynamic cellular processes, and deconvolution microscopy. The specific system for which funding is being requested, and how the capabilities of this system relate to the Aims of the NIH-funded research program of each investigator, are described in detail in the body of this proposal.

描述（由申请人提供）：全细胞以及分离的细胞骨架元件和细胞器的最先进成像对于实现 NIH 授予加州大学六个项目的特定目标至关重要。参与这项共享仪器拨款提案的伯克利细胞生物学家。使用最新技术采集和分析荧光图像对于每个研究项目的持续成功至关重要。该提案的总体目标是获得资金来购买最先进的奥林巴斯成像系统，该系统具有申请人目前无法获得的功能，或者需求量很大，以至于现有共享系统上的时间竞争严重阻碍了进展。该成像系统提供的功能包括全内反射 (TIRF) 显微镜、用于实时分析动态细胞过程的高速多色图像采集以及反卷积显微镜。本提案正文详细描述了正在申请资助的具体系统，以及该系统的功能如何与 NIH 资助的每个研究者的研究计划的目标相关。