Fluorescence microscopy image acquisition/analysis sys

描述（由申请人提供）：最先进的全细胞和分离的细胞骨架元件和细胞器成像对于实现NIH资助的特定目标至关重要，该资助授予参与此共享仪器资助提案的六位加州大学伯克利分校细胞生物学家。使用最新技术获取和分析荧光图像已成为这些研究项目持续成功的关键。该提案的总体目标是确保获得资金，以获得最先进的奥林巴斯成像系统，该系统的功能目前对申请人来说是不可用的，或者对该系统的需求如此之高，以至于由于对现有共享系统的时间竞争而严重阻碍了进展。该成像系统提供的功能包括全内反射（TIRF）显微镜、用于动态细胞过程实时分析的高速多色图像采集和反卷积显微镜。申请资助的具体系统，以及该系统的能力如何与nih资助的每个研究者的研究项目的目标相关联，在本提案的正文中详细描述。

DESCRIPTION (provided by applicant): State-of-the-art imaging of whole cells and of isolated cytoskeletal elements and organelles is vital to achieving the Specific Aims of the NIH grants awarded to each of the six U.C. Berkeley cell biologists participating in this Shared Instrumentation Grant proposal. Acquisition and analysis of fluorescence images using the latest technologies has become essential to the continued success of each of these research programs. The overall aim of this proposal is to secure funds to acquire a state-of-the-art Olympus imaging system with capabilities that are currently unavailable to the applicants, or that are in such high demand that progress is being significantly hindered by competition for time on the one existing shared system. The capabilities to be provided by this imaging system include Total Internal Reflection (TIRF) microscopy, high-speed multi-color image acquisition for real time analysis of dynamic cellular processes, and deconvolution microscopy. The specific system for which funding is being requested, and how the capabilities of this system relate to the Aims of the NIH-funded research program of each investigator, are described in detail in the body of this proposal.