IDENTIFICATION OF NOVEL MEMBERS OF S CEREVISIAE CYTOKINESIS APPARATUS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 由于人类胞质分裂的重要成分在酿酒酵母中高度保守，我正在利用酵母的实验优势来研究胞质分裂。为了将细胞动态环收缩从细胞的相关过程中分离出来，并更好地控制实验参数，我正在开发一种细胞动态环收缩的无细胞功能分析。令人兴奋的是，我发现有可能从酵母中分离出完整的细胞动力装置。为了找到足够的细胞动环收缩机制的确定清单，我正在寻找与部分纯化的、完整的啤酒细胞质分裂装置相关的新因素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As important elements of human cytokinesis are highly conserved in S. cerevisiae, I am exploiting yeast's experimental advantages to study cytokinesis. To isolate cytokinetic ring contraction from related processes of the cell and give greater control of experimental parameters, I am developing a cell free functional assay for cytokinetic ring contraction. Excitingly, I have found it possible to isolate intact cytokinetic apparatus from yeast. To move to a definitive list of machinery sufficient for cytokinetic ring contraction, I am seeking to identify novel factors that are associated with partially purified, intact cerevisiae cytokinesis apparatus.