PROTEIN COMPOSITION OF ACTIN TAILS ASSEMBLED IN YEAST EXTRACTS

酵母提取物中组装的肌动蛋白尾部的蛋白质组成

• 批准号: 8365818
• 负责人: DAVID G DRUBIN
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365818
• 关键词: Actins; Biology; Cells; Clathrin; Complex; Cytosol; Defect; Disease; Endocytosis; Environment; Funding; Fungal Genome; Grant; Laboratories; Life; Mass Spectrum Analysis; Mediating; Membrane; Microfilaments; Microspheres; Molecular; National Center for Research Resources; Polystyrenes; Principal Investigator; Process; Proteins; Reaction; Research; Research Infrastructure; Resources; Role; Saccharomyces cerevisiae; Saccharomycetales; Set protein; Source; Structure; System; Tail; United States National Institutes of Health; Vesicle; Yeasts; base; coated pit; cost; extracellular; in vivo; macromolecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Clathrin-mediated endocytosis is a fundamental process in living cells, allowing them to capture macromolecules efficiently from the extracellular environment and package them into vesicles in the cytosol. Numerous diseases can be traced to a defect in the succession of molecular reactions leading to endocytosis. In the budding yeast Saccharomyces cerevisiae, endocytosis is localized to cortical actin patches, where a dense network of actin filaments is polymerized around clathrin-coated pits. This network is regulated by a complex set of proteins which cooperate in order to generate membrane invagination. We have recently developed in our laboratory a system in yeast extracts for the assembly of actin based structures around polystyrene microbeads. We believe that the protein composition of these structures could be similar to the protein composition found in vivo in cortical actin patches. We isolated the beads with their associated actin structures, and we would like to determine the protein composition by using mass spectroscopy. These results should allow us to further analyze the role of the different components during endocytosis.

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