IDENTIFICATION OF NOVEL MEMBERS OF S CEREVISIAE CYTOKINESIS APPARATUS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 作为人类胞质分裂的重要元件，在S.酿酒酵母，我正在利用酵母的实验优势来研究胞质分裂。 为了从细胞的相关过程中分离细胞动力学环收缩，并更好地控制实验参数，我正在开发一种用于细胞动力学环收缩的无细胞功能测定。 令人兴奋的是，我发现有可能从酵母中分离出完整的细胞动力学装置。 移动到一个明确的清单机械足以胞质环收缩，我正在寻求确定新的因素，与部分纯化，完整的酿酒酵母胞质分裂装置。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As important elements of human cytokinesis are highly conserved in S. cerevisiae, I am exploiting yeast's experimental advantages to study cytokinesis. To isolate cytokinetic ring contraction from related processes of the cell and give greater control of experimental parameters, I am developing a cell free functional assay for cytokinetic ring contraction. Excitingly, I have found it possible to isolate intact cytokinetic apparatus from yeast. To move to a definitive list of machinery sufficient for cytokinetic ring contraction, I am seeking to identify novel factors that are associated with partially purified, intact cerevisiae cytokinesis apparatus.