ENDOCYTOSIS AND CELL CYCLE IN SACCHAROMYCES CEREVISIAE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 最近的活细胞成像研究，加上越来越多的实验遗传学， 生化和药理学数据扩大了我们对这种分子的认识 内吞过程中发生的事件以及肌动蛋白所起的作用(托雷特和德鲁宾， 2006)。在这项合作中，我们的目标是加深对内吞作用的理解 酵母菌利用相关荧光和软x射线断层成像技术对细胞进行成像 一些不同突变的内细胞型的超微结构。这些信息 所获得的结果不仅突出了这些突变对内吞过程的影响 和机器，但确定这些变化对组织的影响 整个细胞。细胞周期是所有生物细胞赖以生存的基本机制 生物体从单细胞细菌复制到多细胞哺乳动物。最多的 细胞周期的基本功能是准确复制细胞中的大量DNA 然后精确地将这些副本分离成完全相同的基因 子代细胞。已经证明，有一个维持比率的一般控制 细胞核体积与细胞体积之比。然而，这种在整个细胞周期中的相关性 还没有完全阐明。此外，很重要的一点是要了解其他 细胞器在细胞周期中是遗传的。此次协作的另一个目标是使用 软X射线断层扫描研究细胞器位置和体积的变化 细胞周期各阶段细胞器的密度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent live-cell imaging studies, coupled with a growing body of experimental genetic, biochemical and pharmacological data have expanded our knowledge of the molecular events that occur during endocytosis and the role played by actin (Toret and Drubin, 2006). In this collaboration we aim to develop a deeper understanding of endocytosis in yeast using correlated fluorescence and soft x-ray tomography to image the cellular ultrastructure of a number of different mutant endocytic phenotypes. The information obtained will not only highlight the effect of these mutations on the endocytic process and machinery, but establish the consequences of these changes on the organization of the entire cell. The cell cycle is the essential mechanism by which the cells of all living organisms from unicellular bacteria to the multicellular mammals duplicate. The most basic function of the cell cycle is to accurately duplicate the vast amount of DNA in the chromosomes and then segregate the copies precisely into genetically identical daughter cells. It has been shown that there is a general control that maintains a ratio of nuclear volume to cell volume. However, such correlation during the entire cell cycle has not been fully elucidated. In addition, it is very important to understand how other organelles are inherited during the cell cycle. Another goal of this collaboration is to use soft xray tomography to study the changes in organelle positioning and the volume and density of organelles during the phases of the cell cycle.