ENDOCYTOSIS AND CELL CYCLE IN SACCHAROMYCES CEREVISIAE

酿酒酵母的内吞作用和细胞周期

• 批准号: 8362731
• 负责人: DAVID G DRUBIN
• 金额: $ 2.94万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362731
• 关键词: Actins; Bacteria; Biochemical; Cell Cycle; Cell Volumes; Cells; Chromosomes; Collaborations; Coupled; DNA; Data; Endocytosis; Event; Experimental Genetics; Fluorescence; Funding; Goals; Grant; Inherited; Knowledge; Life; Mammals; Molecular; Mutation; National Center for Research Resources; Nuclear; Organelles; Organism; Phase; Phenotype; Play; Positioning Attribute; Principal Investigator; Process; Research; Research Infrastructure; Resources; Role; Saccharomyces cerevisiae; Source; United States National Institutes of Health; X-Ray Tomography; Yeasts; cellular imaging; cost; daughter cell; density; mutant; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Recent live-cell imaging studies, coupled with a growing body of experimental genetic, biochemical and pharmacological data have expanded our knowledge of the molecular events that occur during endocytosis and the role played by actin (Toret and Drubin, 2006). In this collaboration we aim to develop a deeper understanding of endocytosis in yeast using correlated fluorescence and soft x-ray tomography to image the cellular ultrastructure of a number of different mutant endocytic phenotypes. The information obtained will not only highlight the effect of these mutations on the endocytic process and machinery, but establish the consequences of these changes on the organization of the entire cell. The cell cycle is the essential mechanism by which the cells of all living organisms from unicellular bacteria to the multicellular mammals duplicate. The most basic function of the cell cycle is to accurately duplicate the vast amount of DNA in the chromosomes and then segregate the copies precisely into genetically identical daughter cells. It has been shown that there is a general control that maintains a ratio of nuclear volume to cell volume. However, such correlation during the entire cell cycle has not been fully elucidated. In addition, it is very important to understand how other organelles are inherited during the cell cycle. Another goal of this collaboration is to use soft xray tomography to study the changes in organelle positioning and the volume and density of organelles during the phases of the cell cycle.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 最近的活细胞成像研究，加上越来越多的实验遗传， 生化和药理学数据扩展了我们对分子的认识 内吞作用过程中发生的事件以及肌动蛋白发挥的作用（Toret 和 Drubin， 2006）。在这次合作中，我们的目标是更深入地了解内吞作用 酵母使用相关荧光和软 X 射线断层扫描对细胞进行成像 许多不同突变内吞表型的超微结构。信息 获得的结果不仅会突出这些突变对内吞过程的影响 和机器，但确定这些变化对组织的影响 整个细胞。细胞周期是所有生物细胞的基本机制 从单细胞细菌到多细胞哺乳动物的生物体都是重复的。最 细胞周期的基本功能是准确地复制细胞中的大量DNA 染色体，然后将副本精确地分离成遗传相同的 子细胞。已经表明，存在一个维持比率的一般控制 核体积与细胞体积之比。然而，在整个细胞周期中这种相关性 尚未完全阐明。此外，了解其他方面的情况也非常重要。 细胞器在细胞周期中遗传。此次合作的另一个目标是利用 软 X 射线断层扫描用于研究细胞器定位和体积的变化 细胞周期各阶段细胞器的密度。