NIMH Center Repository Supporting Stem Cell Research

描述(申请人提供)：本修订U24 L\/lH068457-08，“国家精神障碍合作研究中心”(国家精神疾病研究中心)申请资助“支持与精神障碍相关的干细胞研究的资料库”，以回应NOT-MH-10-024。自1998年底以来，NIMHC建立了一个强大的基础设施，用于接收血液样本和外部生产的细胞培养。NIMHC的生物储存库位于罗格斯大学，其数据储存库位于华盛顿大学医学院。已经从血液中提取了DNA和RNA，并为NIMHC内部培养了超过75,000个淋巴母细胞系，第一次尝试的成功率为99.8%。在过去的5年里，NIMHC向世界各地的研究人员分发了450,000个DNA，15,000个RNA和8,000个细胞系。基于满足NIMH遗传学计划受赠者的需求，NIMHC维护着安全的临床(表型)和基因组数据库，以及一个受密码保护的安全网站，供NIMH批准的研究人员访问。NIMHC还开发并提供了基于网络的生物信息学资源，用于精神病学遗传学研究的设计、分析和解释。我们建议利用NIMHC的设施、专业知识和经验为希望利用人类患者和控制原代细胞、成体干细胞和诱导多能干细胞(IPSCs)研究精神障碍的细胞基础的研究人员提供服务。NIMHC将接收用于建立和储存原代细胞培养或成体干细胞系的原始组织样本。NIMHC还将接受原代细胞、成体干细胞和IPSCs的培养，用于鉴定和储存。这些细胞系以及描述细胞系的临床和基因组数据以及分子和表型数据将被分发给NIMH批准的研究人员。NIMHC还将就这些干细胞系的性质和用途提供咨询。在较小的程度上，NIMHC还将生产、鉴定和储存由原代细胞制成的IPSC。在研究界内自由共享原代细胞和干细胞可能会极大地加快理解精神障碍的细胞基础的进展。 公共卫生相关性：精神障碍是一种常见、广泛和不同种类的疾病，其生物学基础尚不清楚。阐明这些疾病的潜在遗传和生理基础对于创造有效的治疗方法是必要的。神经细胞或来自成人干细胞的其他细胞提供了在细胞培养和药物初步筛选中对这些疾病进行建模的可能性。

DESCRIPTION (provided by applicant): This revision of U24 l\/lH068457-08, "NIMH Center for Collaborative Studies on Mental Disorders," (NIMHC) requests funding for a "Repository Supporting Stem Cell Research Relevant to Mental Disorders" in response to NOT-MH-10-024. Since late 1998, the NIMHC, whose biorepository is at Rutgers University and whose data repository is at Washington University School of Medicine, has established a robust infrastructure for the receipt of blood samples and externally produced cell cultures. DNA and RNA has been extracted from blood and over 75,000 lymphoblastoid cell lines have been produced in-house for the NIMHC, with 99.8% success on the first attempt. In the past 5 years, the NIMHC has distributed -450,000 DNA, ~15,000 RNA and ~8,000 cell lines to researchers throughout the world. Based on serving the needs of NIMH Genetics Initiative grantees, the NIMHC maintains secure clinical (phenotypic) and genomic databases and a password protected, secure website for NIMH-approved researchers to access. The NIMHC has also developed and made available web-based bioinformatics resources for the design, analysis and interpretation of studies in psychiatric genetics. We propose to utilize the facilities, expertise and experience of the NIMHC to serve researchers wishing to use human patient and control primary cells, adult stem cells and induced pluripotent stem cells (iPSCs) for investigation of the cellular bases of mental disorders. The NIMHC will receive primary tissue samples for the establishment and banking of primary cell cultures or adult stem cell lines. The NIMHC will also receive cultures of primary cells, adult stem cells and iPSCs for characterization and banking. These cell lines, along with subject clinical and genomic data and molecular and phenotypic data describing the cell lines, will be distributed to NIMH-approved researchers. The NIMHC will also provide consultation regarding the nature and use of these stem cell lines. To a lesser extent, the NIMHC will also produce, characterize and bank iPSCs made from primary cells. Freely sharing primary and stem cells within the research community is likely to greatly accelerate progress in understanding the cellular bases of mental disorders. Public Health Relevance: Mental disorders are a common, widespread and heterogeneous group of diseases whose biological bases are poorly understood. Elucidation of the underlying genetic and physiological bases for these disorders is necessary for the creation of effective therapeutics. Nerve cells or other cells derived from adult stem cells offer the possibility of modeling these disorders in cell culture and for preliminary screening of pharmaceuticals.