Measuring Entomological Risk for Dengue

测量登革热的昆虫学风险

• 批准号: 7087286
• 负责人: THOMAS WALLACE SCOTT
• 金额: $ 50.22万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7087286
• 关键词: Aedes; DNA; Peru; blood; clinical research; dengue virus; household; human; immunity; infection; measurement; serotyping; temperature; urban area

DESCRIPTION (provided by applicant): The impact of human movement patterns on exposure of people to bites from mosquito vectors is a major knowledge gap in the understanding of dengue virus (DV) transmission dynamics. Because Ae. aegypti is a daytime biting mosquito we anticipate that the daytime activity patterns of human hosts will profoundly affect their risk of exposure to infective mosquito bites. Testing these ideas will require identification of operationally feasible measures of entomological risk estimated at an appropriate geographic scale that accounts for movements of susceptible humans in and out of high risk areas. Relative risk of DV infection has never been rigorously measured at the multiple locations that susceptible individual visit during their daily activities; like the homes of neighbors or relatives, schools, markets, workplaces or other communities. In the Amazonian city of Iquitos, Peru where we have over 6 years of Ae. aegypti and dengue research experience we propose to test the hypothesis that spatial dimensions of dengue virus transmission are defined by daily patterns of human movement. We predict that DV incidence rates are positively correlated with the number of bites received from Aedes aegypti at high risk locations, serotype specific herd immunity, and ambient temperature. We intend to test our hypothesis in the context of 2 specific aims. First, a combination of retrospective and prospective cohort studies will be used to determine whether measurement of entomological risk across a person's activity space provides a better prediction of DV infection risk than limiting assessment to their household. Second, we will use DNA profiling of blood in engorged mosquitoes to determine the risk of a person being bitten by Ae. aegypti at different sites across their activity space. Data collected in these field studies will be analyzed using a series of spatial statistical techniques and social network modeling to define the spatial dimensions of DV transmission and the predictive value of entomological surveillance. Our goal is to clarify the value of entomological indices in dengue surveillance programs by defining the spatial dimensions of DV transmission. Our research will be the most definitive test to-date of the empirical relationship between entomological risk and DV transmission. Our results will be relevant for dengue prevention programs based on vector control and/or vaccine strategies and will have immediate, practical applications for dengue surveillance and control worldwide.

描述(申请人提供)：人类活动模式对人们接触蚊媒叮咬的影响是理解登革热病毒(DV)传播动力学的一个主要知识空白。因为Ae.埃及伊蚊是一种白天叮咬的蚊子，我们预计人类宿主的白天活动模式将深刻影响他们接触感染蚊子叮咬的风险。测试这些想法将需要确定在适当的地理范围内估计的昆虫学风险的操作上可行的措施，以说明易感人群进出高风险地区的情况。在日常活动中易受感染的多个地点，如邻居或亲戚的家中、学校、市场、工作场所或其他社区，从未严格衡量过DV感染的相对风险。在秘鲁的亚马逊河城市伊基托斯，我们有超过6年的Ae。埃及伊蚊和登革热的研究经验，我们建议检验登革热病毒传播的空间维度由人类日常运动模式定义的假设。我们预测，DV发病率与高危地区埃及伊蚊叮咬次数、血清型特异性群体免疫和环境温度呈正相关。我们打算在两个具体目标的背景下检验我们的假设。首先，将使用回顾和前瞻性队列研究相结合的方法来确定，衡量一个人的活动空间的昆虫学风险是否比将评估局限于其家庭更能更好地预测DV感染风险。其次，我们将使用充血蚊子血液的DNA图谱来确定一个人被Ae叮咬的风险。埃及伊蚊在不同地点的活动空间。在这些实地研究中收集的数据将利用一系列空间统计技术和社会网络建模进行分析，以确定DV传播的空间维度和昆虫学监测的预测价值。我们的目标是通过定义登革热传播的空间维度来阐明昆虫学指数在登革热监测项目中的价值。我们的研究将是迄今为止对昆虫学风险和DV传播之间的经验关系的最明确的检验。我们的结果将与基于病媒控制和/或疫苗战略的登革热预防计划相关，并将立即在世界各地的登革热监测和控制中应用。