Measuring Entomological Risk for Dengue

测量登革热的昆虫学风险

• 批准号: 7087286
• 负责人: THOMAS WALLACE SCOTT
• 金额: $ 50.22万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7087286
• 关键词: Aedes; DNA; Peru; blood; clinical research; dengue virus; household; human; immunity; infection; measurement; serotyping; temperature; urban area

DESCRIPTION (provided by applicant): The impact of human movement patterns on exposure of people to bites from mosquito vectors is a major knowledge gap in the understanding of dengue virus (DV) transmission dynamics. Because Ae. aegypti is a daytime biting mosquito we anticipate that the daytime activity patterns of human hosts will profoundly affect their risk of exposure to infective mosquito bites. Testing these ideas will require identification of operationally feasible measures of entomological risk estimated at an appropriate geographic scale that accounts for movements of susceptible humans in and out of high risk areas. Relative risk of DV infection has never been rigorously measured at the multiple locations that susceptible individual visit during their daily activities; like the homes of neighbors or relatives, schools, markets, workplaces or other communities. In the Amazonian city of Iquitos, Peru where we have over 6 years of Ae. aegypti and dengue research experience we propose to test the hypothesis that spatial dimensions of dengue virus transmission are defined by daily patterns of human movement. We predict that DV incidence rates are positively correlated with the number of bites received from Aedes aegypti at high risk locations, serotype specific herd immunity, and ambient temperature. We intend to test our hypothesis in the context of 2 specific aims. First, a combination of retrospective and prospective cohort studies will be used to determine whether measurement of entomological risk across a person's activity space provides a better prediction of DV infection risk than limiting assessment to their household. Second, we will use DNA profiling of blood in engorged mosquitoes to determine the risk of a person being bitten by Ae. aegypti at different sites across their activity space. Data collected in these field studies will be analyzed using a series of spatial statistical techniques and social network modeling to define the spatial dimensions of DV transmission and the predictive value of entomological surveillance. Our goal is to clarify the value of entomological indices in dengue surveillance programs by defining the spatial dimensions of DV transmission. Our research will be the most definitive test to-date of the empirical relationship between entomological risk and DV transmission. Our results will be relevant for dengue prevention programs based on vector control and/or vaccine strategies and will have immediate, practical applications for dengue surveillance and control worldwide.

描述（由申请方提供）：人类活动模式对人类暴露于蚊媒叮咬的影响是了解登革热病毒（DV）传播动力学的一个主要知识空白。因为爱埃及伊蚊是一种白天叮咬的蚊子，我们预期人类宿主的日间活动模式会严重影响他们被蚊子叮咬的风险。要检验这些想法，就需要确定在适当的地理尺度上估计的昆虫学风险的操作上可行的措施，这种尺度要考虑到易感人群进出高风险地区的情况。DV感染的相对风险从未在易感个体在日常活动中访问的多个地点进行过严格测量;如邻居或亲戚的家，学校，市场，工作场所或其他社区。在秘鲁的亚马逊城市伊基托斯，我们有超过6年的Ae。埃及和登革热的研究经验我们建议检验登革热病毒传播的空间维度由人类活动的日常模式确定这一假设。我们预测DV发病率与在高风险地点收到的埃及伊蚊叮咬次数、血清型特异性群体免疫力和环境温度呈正相关。我们打算在两个具体目标的背景下测试我们的假设。首先，将使用回顾性和前瞻性队列研究相结合的方法来确定是否在一个人的活动空间内测量昆虫学风险比限制对其家庭的评估更好地预测DV感染风险。其次，我们将使用充血蚊子血液的DNA分析来确定一个人被Ae叮咬的风险。埃及人在其活动空间的不同地点。在这些实地研究中收集的数据将使用一系列的空间统计技术和社会网络建模进行分析，以确定DV传播的空间维度和昆虫监测的预测价值。我们的目标是通过定义DV传播的空间维度来阐明昆虫学指标在登革热监测计划中的价值。我们的研究将是迄今为止对昆虫学风险与DV传播之间的经验关系的最确定的测试。我们的研究结果将与基于病媒控制和/或疫苗策略的登革热预防计划相关，并将对全球登革热监测和控制产生直接的实际应用。