Network for Premature Birth Research Analytical Core

早产研究分析核心网络

• 批准号: 6943666
• 负责人: SAMUEL I. PARRY
• 金额: $ 84.07万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6943666
• 关键词: bioinformatics; biomarker; biomedical resource; biotechnology; cervical /vaginal smear; clinical research; cooperative study; functional /structural genomics; genetic markers; high throughput technology; human genetic material tag; human subject; linkage mapping; microarray technology; nucleic acid amplification techniques; oxidative stress; premature infant human; premature labor; proteomics; statistics /biometry; women' s health

DESCRIPTION (provided by applicant): This proposal is submitted to create an Analytical Core, which will serve the Genomic and Proteomics Network for Premature Birth Research. The Analytical Core will have five components that will meet the needs for 1) sample processing, storage and distribution/retrieval; 2) high-throughput genotyping and interpretation of genetic studies; 3) microarray analysis and data mining; 4) proteomics, lipidomics and assessment of oxidative stress; and 5) Bioinformatics and statistics. Each component of this Core will be supervised by an established investigator with expertise in the specific methodology and data interpretation. The overall coordination of Analytical Core 1 functions will be the responsibility of the Principal Investigator who will also serve as the Director of the sample processing, storage, distribution/retrieval component and administrative unit (Jerome F. Strauss, III, M.D., Ph.D.), working in concert with the component facility directors (Genotyping and genetic analysis: Richard S. Spielman, Ph.D.; Microarray: Don Baldwin Ph.D.; Proteomics/lipidomics and oxidative stress: Ian Blair, Ph.D.: Bioinformatics and statistics: David Fenstermacher, Ph.D.) who will serve as the Analytical Core Steering Committee. The Principal Investigator will serve as the Steering Committee Chair and the interface with the other investigators and N.I.C.H.D. staff participating in this program. The proposed Core builds on established strengths of the University of Pennsylvania in the study of the genetics of preterm birth, the genetics of complex traits, proteomics, the study of oxidative stress biomarkers, and microarray analysis. The existing facilities are prepared to accommodate high-throughput assays. In addition, the Core key personnel can provide expert advice in study design and data analysis. A Concept Protocol built around the use of admixture mapping and the transmission disequilibrium test to identify the impact of ancestry on preterm birth and neonatal outcomes after preterm delivery as well as association and linkage studies to identify specific risk promoting and protective genes, and parent of origin effects.

描述（由申请人提供）：提交该提案以创建一个分析核心，该核心将为早产研究提供基因组和蛋白质组学网络。分析核心将有五个组件满足1）样品处理，存储和分配/检索的需求； 2）遗传研究的高通量基因分型和解释； 3）微阵列分析和数据挖掘； 4）蛋白质组学，脂质组学和氧化应激的评估； 5）生物信息学和统计。 该核心的每个组成部分将由一位既定的研究人员进行监督，该调查员在特定方法和数据解释方面具有专业知识。 分析核心1功能的总体协调将是主要研究人员的责任，他还将担任样品处理，存储，分销/检索和行政部门（Jerome F. Strauss，III，M.D.，M.D.，Ph.D.）的主任，与组件设施董事合作（基因分析和遗传分析：Richard S. Spiel S. Spiel S. ph.； Phord。蛋白质组学/脂肪组学和氧化压力：Ian Blair博士：生物信息学和统计学：David Fenstermacher，Ph.D。），他将担任分析核心指导委员会。首席调查员将担任指导委员会主席以及与其他调查人员和N.I.C.H.D.的界面。参加此计划的工作人员。提出的核心是基于宾夕法尼亚大学的既定优势，研究了早产，复杂性状，蛋白质组学，氧化应激生物标志物的研究和微阵列分析的研究。现有的设施准备适应高通量测定法。此外，核心关键人员可以在研究设计和数据分析方面提供专家建议。一种概念方案围绕使用混合图和传播不平衡测试构建，以确定祖先对早产后的早产和新生儿结局的影响，以及结合和链接研究，以识别特定的风险促进和保护性基因，以及对原始效应的父母。