Network for Premature Birth Research Analytical Core

描述(由申请人提供)：本提案旨在创建一个分析核心，该核心将为早产研究基因组和蛋白质组学网络提供服务。分析核心将由五个部分组成，将满足以下需要：1)样品处理、储存和分配/检索；2)高通量基因分型和基因研究解释；3)微阵列分析和数据挖掘；4)蛋白质组学、脂类组学和氧化应激评估；以及5)生物信息学和统计学。这一核心的每个组成部分都将由一名在具体方法和数据解释方面具有专门知识的常设调查员监督。分析核心1职能的全面协调将由首席调查员负责，他还将担任样品处理、储存、分配/回收组件和管理单位的主任(Jerome F.Strauss，III，M.D.，Ph.D.)，与组件设施主任(基因分型和基因分析：Richard S.Spielman，Ph.D.；微阵列：Don Baldwin Ph.D.；蛋白质组学/脂质组学和氧化应激：Ian Blair，Ph.D.：生物信息学和统计学：David Fenstermacher，Ph.D.)合作。世卫组织将担任分析核心指导委员会。首席调查员将担任指导委员会主席，并与参与该计划的其他调查人员和NICHD工作人员进行联系。拟议的核心建立在宾夕法尼亚大学在早产遗传学、复杂性状遗传学、蛋白质组学、氧化应激生物标志物研究和微阵列分析方面的既定优势的基础上。现有的设施已准备好适应高通量的分析。此外，核心关键人员可以在研究设计和数据分析方面提供专家建议。基于混合图谱和传递不平衡检验建立的概念方案，以确定血统对早产和早产后新生儿结局的影响，以及关联和连锁研究，以确定特定的风险促进和保护基因，以及起源父母的影响。

DESCRIPTION (provided by applicant): This proposal is submitted to create an Analytical Core, which will serve the Genomic and Proteomics Network for Premature Birth Research. The Analytical Core will have five components that will meet the needs for 1) sample processing, storage and distribution/retrieval; 2) high-throughput genotyping and interpretation of genetic studies; 3) microarray analysis and data mining; 4) proteomics, lipidomics and assessment of oxidative stress; and 5) Bioinformatics and statistics. Each component of this Core will be supervised by an established investigator with expertise in the specific methodology and data interpretation. The overall coordination of Analytical Core 1 functions will be the responsibility of the Principal Investigator who will also serve as the Director of the sample processing, storage, distribution/retrieval component and administrative unit (Jerome F. Strauss, III, M.D., Ph.D.), working in concert with the component facility directors (Genotyping and genetic analysis: Richard S. Spielman, Ph.D.; Microarray: Don Baldwin Ph.D.; Proteomics/lipidomics and oxidative stress: Ian Blair, Ph.D.: Bioinformatics and statistics: David Fenstermacher, Ph.D.) who will serve as the Analytical Core Steering Committee. The Principal Investigator will serve as the Steering Committee Chair and the interface with the other investigators and N.I.C.H.D. staff participating in this program. The proposed Core builds on established strengths of the University of Pennsylvania in the study of the genetics of preterm birth, the genetics of complex traits, proteomics, the study of oxidative stress biomarkers, and microarray analysis. The existing facilities are prepared to accommodate high-throughput assays. In addition, the Core key personnel can provide expert advice in study design and data analysis. A Concept Protocol built around the use of admixture mapping and the transmission disequilibrium test to identify the impact of ancestry on preterm birth and neonatal outcomes after preterm delivery as well as association and linkage studies to identify specific risk promoting and protective genes, and parent of origin effects.