Network for Premature Birth Research Analytical Core

描述（由申请人提供）：提交此提案是为了创建一个分析核心，该分析核心将服务于早产研究的基因组和蛋白质组学网络。分析核将有五个组成部分，以满足以下需求：1)样品处理、储存和分发/检索；2)高通量基因分型和遗传研究解释；3)微阵列分析与数据挖掘；4)蛋白质组学、脂质组学与氧化应激评估；5)生物信息学与统计学。该核心的每个组成部分将由具有特定方法和数据解释专业知识的既定调查员监督。分析核心1功能的整体协调将由首席研究员负责，他也将担任样品处理，储存，分发/检索组件和管理单元的主任（Jerome F. Strauss， III, m.d., Ph.D.），与组件设施主任(基因分型和遗传分析：Richard S. Spielman博士，微阵列：Don Baldwin博士；蛋白质组学/脂质组学和氧化应激：Ian Blair博士；生物信息学和统计学：David Fenstermacher博士)，他将担任分析核心指导委员会。首席研究员将担任指导委员会主席，并与参与该项目的其他研究人员和N.I.C.H.D.工作人员进行沟通。拟议的核心建立在宾夕法尼亚大学在早产遗传学研究、复杂性状遗传学、蛋白质组学、氧化应激生物标志物研究和微阵列分析方面的既定优势之上。现有设施已准备好进行高通量分析。此外，核心关键人员可以在研究设计和数据分析方面提供专家意见。一项概念方案建立在使用混合制图和传播不平衡测试，以确定血统对早产和早产后新生儿结局的影响，以及关联和连锁研究，以确定特定的风险促进和保护基因，以及父母的起源效应。

DESCRIPTION (provided by applicant): This proposal is submitted to create an Analytical Core, which will serve the Genomic and Proteomics Network for Premature Birth Research. The Analytical Core will have five components that will meet the needs for 1) sample processing, storage and distribution/retrieval; 2) high-throughput genotyping and interpretation of genetic studies; 3) microarray analysis and data mining; 4) proteomics, lipidomics and assessment of oxidative stress; and 5) Bioinformatics and statistics. Each component of this Core will be supervised by an established investigator with expertise in the specific methodology and data interpretation. The overall coordination of Analytical Core 1 functions will be the responsibility of the Principal Investigator who will also serve as the Director of the sample processing, storage, distribution/retrieval component and administrative unit (Jerome F. Strauss, III, M.D., Ph.D.), working in concert with the component facility directors (Genotyping and genetic analysis: Richard S. Spielman, Ph.D.; Microarray: Don Baldwin Ph.D.; Proteomics/lipidomics and oxidative stress: Ian Blair, Ph.D.: Bioinformatics and statistics: David Fenstermacher, Ph.D.) who will serve as the Analytical Core Steering Committee. The Principal Investigator will serve as the Steering Committee Chair and the interface with the other investigators and N.I.C.H.D. staff participating in this program. The proposed Core builds on established strengths of the University of Pennsylvania in the study of the genetics of preterm birth, the genetics of complex traits, proteomics, the study of oxidative stress biomarkers, and microarray analysis. The existing facilities are prepared to accommodate high-throughput assays. In addition, the Core key personnel can provide expert advice in study design and data analysis. A Concept Protocol built around the use of admixture mapping and the transmission disequilibrium test to identify the impact of ancestry on preterm birth and neonatal outcomes after preterm delivery as well as association and linkage studies to identify specific risk promoting and protective genes, and parent of origin effects.