Regulation of Energy Metabolism in Insects

昆虫能量代谢的调节

• 批准号: 7048021
• 负责人: ROGER L MIESFELD
• 金额: $ 33.93万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7048021
• 关键词: Aedes; Anopheles; aminoacid metabolism; bioenergetics; carbon dioxide; dietary aminoacid; enzyme activity; female; glycogen; glycogenolysis; lipids; nutrition; nutrition related tag; oogenesis; phosphoenolpyruvate carboxylase; protein metabolism

DESCRIPTION (provided by applicant): The results obtained so far with Ae. aegypti represent the first dynamic approach to determining the fate of amino acids from meal proteins in mosquitoes. Using the methods developed during the last grant period, we are now in a position to probe the metabolic control of amino acid metabolism in more detail and to begin an analysis of metabolic fuel accretion during larval development, as well as, applying these methods to other mosquitoes, such as Culex pipiens. The ultimate aim of this project is to define blood meal protein amino acid metabolism in the context of the whole mosquito and to understand, in quantitative terms, how the different metabolic fluxes simultaneously support the gonotrophic cycle after a blood meal and the survival of the female mosquito, because unless she survives the first gonotrophic cycle there would be no opportunity to take a second blood meal and transmit disease. Such an analysis offers a unique opportunity to identify new and unexpected targets for mosquito control. The following specific aims are proposed for the next grant period: Aim 1: Investigate the metabolic fates of blood meal amino acids in Ae. aegypti females. How is the mosquito able to selectively alter the metabolic fate of different amino acids? What are the metabolic fates of proline, the major hemolymph amino acid, during blood meal digestion? How does the metabolism of ammonia released from amino acid oxidation change during a gonotrophic cycle? Aim 2: Develop quantitative models that accurately describe metabolic flux in Ae. aegypti females. What model best describes the time course for amino acid metabolism during the gonotrophic cycle? How can similar quantitative models be applied to understanding whole mosquito metabolism? Aim 3: Investigate the dynamics of metabolic flux during development in Ae. aegypti. How is the metabolism of larvae integrated to ensure the synthesis of energy reserves? Aim 4 Determine metabolic changes that occur in specific tissues of Ae. aegypti females. What is the extent of protein turnover under different metabolic conditions? How does the metabolism of the fat body and midgut change during the gonotrophic cycle? Aim 5: Investigate the dynamics of metabolic flux during diapause in C. pipiens. How does diapause alter sugar metabolism in C. pipiens? What is the fate of reserves during diapause in C. pipiens?

描述（由申请人提供）：迄今为止使用Ae. Aegypti代表了确定蚊子中来自膳食蛋白质的氨基酸命运的第一种动态方法。使用在最后一个赠款期间开发的方法，我们现在能够更详细地探索氨基酸代谢的代谢控制，并开始分析幼虫发育过程中的代谢燃料积累，以及将这些方法应用于其他蚊子，如库蚊。该项目的最终目的是在整个蚊子的背景下定义血餐蛋白质氨基酸代谢，并以定量的方式了解不同的代谢通量如何同时支持血餐后的生殖营养周期和雌蚊的生存，因为除非她在第一个生殖营养周期中幸存下来，否则就没有机会服用第二次血餐并传播疾病。这种分析为确定新的和意想不到的蚊子控制目标提供了独特的机会。以下具体目标，提出了下一个授权期：目标1：调查的代谢命运的血粉氨基酸在Ae。埃及女性。 蚊子是如何选择性地改变不同氨基酸的代谢命运的？ 在血餐消化过程中，主要血淋巴氨基酸脯氨酸的代谢命运是什么？ 在生殖营养循环中，氨基酸氧化释放的氨的代谢是如何变化的？目的2：开发定量模型，准确地描述代谢通量在Ae。埃及女性。 什么模型最能描述生殖营养周期中氨基酸代谢的时间进程？ 如何将类似的定量模型应用于了解整个蚊子代谢？目的3：研究山羊草发育过程中代谢流的动态变化。埃及人。 幼虫的新陈代谢是如何整合的，以保证能量储备的合成？目的4确定Ae特定组织的代谢变化。埃及女性。 在不同的代谢条件下，蛋白质周转的程度如何？在性腺营养周期中，脂肪体和中肠的代谢是如何变化的？目的5：研究滞育过程中代谢流的动态变化。pipiens。 滞育如何改变C的糖代谢。pipiens？ 在滞育期间，C. pipiens？