Regulation of Energy Metabolism in Insects

昆虫能量代谢的调节

• 批准号: 7559707
• 负责人: ROGER L MIESFELD
• 金额: $ 32.36万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7559707
• 关键词: Adult; Aedes; Amino Acids; Ammonia; Blood; Carbon; Carbon Dioxide; Complex; Culex (Genus); Culicidae; Development; Diapause; Digestion; Disease; Energy Metabolism; Ensure; Fat Body; Fatty Acids; Female; Future; Generations; Glucose; Glutamine; Glycogen; Goals; Grant; Hemolymph; Insecta; Label; Larva; Lipids; Metabolic; Metabolic Control; Metabolism; Methods; Midgut; Modeling; Mosquito Control; Pathway interactions; Play; Positioning Attribute; Production; Proline; Protein Biosynthesis; Proteins; Reaction; Regulation; Research Personnel; Role; Staging; Time; Tissues; Trehalose; Vitellogenesis; base; carbohydrate metabolism; egg; lipid biosynthesis; metabolic abnormality assessment; oxidation; programs; protein degradation; sugar

The results obtained so far with Ae. aegypti represent the first dynamic approach to determining the fate of amino acids from meal proteins in mosquitoes. Using the methods developed during the last grant period, we are now in a position to probe the metabolic control of amino acid metabolism in more detail and to begin an analysis of metabolic fuel accretion during larval development, as well as, applying these methods to other mosquitoes, such as Culex pipens. The ultimate aim of this project is to define blood meal protein amino acid metabolism in the context of the whole mosquito and to understand, in quantitative terms, how the different metabolic fluxes simultaneously support the gonotrophic cycle after a blood meal and the survival of the female mosquito, because unless she survives the first gonotrophic cycle there would be no opportunity to take a second blood meal and transmit disease. Such an analysis offers a unique opportunity to identify new and unexpected targets for mosquito control. The following specific aims are proposed for the next grant period: Aim 1 Investigate the metabolic fates of blood meal amino acids in Ae. aegypti females. ¿ How is the mosquito able to selectively alter the metabolic fate of different amino acids? ¿ What are the metabolic fates of proline, the major hemolymph amino acid, during blood meal digestion? ¿ How does the metabolism of ammonia released from amino acid oxidation change during a gonotrophic cycle? Aim 2 Develop quantitative models that accurately describe metabolic flux in Ae. aegypti females. ¿ What model best describes the time course for amino acid metabolism during the gonotrophic cycle? ¿ Howcan similar quantitative models be applied to understanding whole mosquito metabolism? Aim 3 Investigate the dynamics of metabolic flux during development in Ae. aegypti. ¿ How is the metabolism of larvae integrated to ensure the synthesis of energy reserves? Aim 4 Determine metabolic changes that occur in specific tissues ofAe. aegypti females. ¿ What is the extent of protein turnover under different metabolic conditions? ¿ Howdoes the metabolism of the fat body and midgut change during the gonotrophic cycle? Aim 5 Investigate the dynamics of metabolic flux during diapause in C.pipiens. ¿ Howdoes diapause alter sugar metabolism in C.pipiens? ¿ What is the fate of reserves during diapause in C.pipiens?

迄今为止使用Ae获得的结果。埃及代表了第一个动态的方法来确定命运， 从蚊子的食物蛋白中提取氨基酸。使用在上一个赠款期间开发的方法，我们 现在能够更详细地探索氨基酸代谢的代谢控制，并开始一项 分析代谢燃料的增长在幼虫发育过程中，以及，应用这些方法，以其他 蚊子，如库蚊。本项目的最终目的是确定血粉蛋白质氨基酸 在整个蚊子的背景下进行新陈代谢，并从数量上了解不同的蚊子 代谢通量同时支持血餐后的生殖营养循环和 雌性蚊子，因为除非她在第一个生殖营养周期中幸存下来，否则就没有机会 吸食第二次血液传播疾病这种分析提供了一个独特的机会，以确定新的 和意想不到的蚊子控制目标。 为下一个赠款期提出了以下具体目标： 目的1研究山羊血粉氨基酸的代谢归宿。埃及女性。 蚊子是如何选择性地改变不同氨基酸的代谢命运的？ 在血餐期间，主要血淋巴氨基酸脯氨酸的代谢命运是什么？ 消化？ <$如何从氨基酸氧化释放的氨的代谢变化过程中， 生殖营养循环 目的2建立准确描述Ae代谢流的定量模型。埃及女性。 什么模型最好地描述了性腺营养周期中氨基酸代谢的时间过程？ 如何将类似的定量模型应用于了解整个蚊子代谢？ 目的3研究山羊草发育过程中代谢流的动态变化。埃及人。 <$如何代谢的幼虫集成，以确保合成的能量储备？ 目的4确定Ae特定组织中发生的代谢变化。埃及女性。 在不同的代谢条件下，蛋白质周转的程度如何？ 在性腺营养周期中，脂肪体和中肠的代谢是如何变化的？ 目的5研究尖音瓢虫滞育期间代谢流的动态。 滞育如何改变尖音隐翅虫的糖代谢？ 在C.pipiens滞育期间储备的命运是什么？