Regulation of Energy Metabolism in Insects

昆虫能量代谢的调节

• 批准号: 7758322
• 负责人: ROGER L MIESFELD
• 金额: $ 32.04万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2012-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7758322
• 关键词: Adult; Aedes; Amino Acids; Ammonia; Blood; Carbon; Carbon Dioxide; Complex; Culex (Genus); Culicidae; Development; Diapause; Digestion; Disease; Energy Metabolism; Ensure; Fat Body; Fatty Acids; Female; Future; Generations; Glucose; Glutamine; Glycogen; Goals; Grant; Hemolymph; Insecta; Label; Larva; Lipids; Metabolic; Metabolic Control; Metabolism; Methods; Midgut; Modeling; Mosquito Control; Pathway interactions; Play; Positioning Attribute; Production; Proline; Protein Biosynthesis; Proteins; Reaction; Regulation; Research Personnel; Role; Staging; Time; Tissues; Trehalose; Vitellogenesis; base; carbohydrate metabolism; egg; lipid biosynthesis; metabolic abnormality assessment; oxidation; programs; protein degradation; sugar

The results obtained so far with Ae. aegypti represent the first dynamic approach to determining the fate of amino acids from meal proteins in mosquitoes. Using the methods developed during the last grant period, we are now in a position to probe the metabolic control of amino acid metabolism in more detail and to begin an analysis of metabolic fuel accretion during larval development, as well as, applying these methods to other mosquitoes, such as Culex pipens. The ultimate aim of this project is to define blood meal protein amino acid metabolism in the context of the whole mosquito and to understand, in quantitative terms, how the different metabolic fluxes simultaneously support the gonotrophic cycle after a blood meal and the survival of the female mosquito, because unless she survives the first gonotrophic cycle there would be no opportunity to take a second blood meal and transmit disease. Such an analysis offers a unique opportunity to identify new and unexpected targets for mosquito control. The following specific aims are proposed for the next grant period: Aim 1 Investigate the metabolic fates of blood meal amino acids in Ae. aegypti females. ¿ How is the mosquito able to selectively alter the metabolic fate of different amino acids? ¿ What are the metabolic fates of proline, the major hemolymph amino acid, during blood meal digestion? ¿ How does the metabolism of ammonia released from amino acid oxidation change during a gonotrophic cycle? Aim 2 Develop quantitative models that accurately describe metabolic flux in Ae. aegypti females. ¿ What model best describes the time course for amino acid metabolism during the gonotrophic cycle? ¿ Howcan similar quantitative models be applied to understanding whole mosquito metabolism? Aim 3 Investigate the dynamics of metabolic flux during development in Ae. aegypti. ¿ How is the metabolism of larvae integrated to ensure the synthesis of energy reserves? Aim 4 Determine metabolic changes that occur in specific tissues ofAe. aegypti females. ¿ What is the extent of protein turnover under different metabolic conditions? ¿ Howdoes the metabolism of the fat body and midgut change during the gonotrophic cycle? Aim 5 Investigate the dynamics of metabolic flux during diapause in C.pipiens. ¿ Howdoes diapause alter sugar metabolism in C.pipiens? ¿ What is the fate of reserves during diapause in C.pipiens?

迄今为止用 Ae 获得的结果。 aegypti 代表了第一个决定命运的动态方法 来自蚊子膳食蛋白质的氨基酸。使用上次资助期间开发的方法，我们 现在能够更详细地探讨氨基酸代谢的代谢控制，并开始 分析幼虫发育过程中代谢燃料的积累，以及将这些方法应用于其他 蚊子，例如尖色库蚊。该项目的最终目标是定义血粉蛋白氨基酸 整个蚊子的新陈代谢，并从数量上理解不同的蚊子之间的差异 代谢通量同时支持血餐后的促性腺营养循环和细胞的存活 雌性蚊子，因为除非她能在第一个性营养周期中存活下来，否则就没有机会 吃第二次血并传播疾病。这种分析提供了一个独特的机会来识别新的 以及意想不到的蚊子控制目标。 为下一个资助期提出以下具体目标： 目标 1 研究伊蚊中血粉氨基酸的代谢命运。埃及伊蚊雌性。 ¿ 蚊子如何能够选择性地改变不同氨基酸的代谢命运？ ¿ 脯氨酸（主要血淋巴氨基酸）在血粉期间的代谢命运如何 消化？ ¿ 氨基酸氧化释放的氨的代谢在一个过程中如何变化？ 性腺营养周期？ 目标 2 开发准确描述伊蚊代谢通量的定量模型。埃及伊蚊雌性。 ¿ 什么模型最能描述性营养循环期间氨基酸代谢的时间进程？ ¿ 如何应用类似的定量模型来理解整个蚊子的新陈代谢？ 目标 3 研究 Ae 发育过程中代谢流的动态。埃及伊蚊。 ¿ 幼虫的新陈代谢是如何整合来保证能量储备的合成的？ 目标 4 确定伊蚊特定组织中发生的代谢变化。埃及伊蚊雌性。 ¿ 不同代谢条件下蛋白质周转的程度是多少？ ¿ 脂肪体和中肠的代谢在促性腺营养周期中如何变化？ 目标 5 研究 C.pipiens 滞育期间代谢通量的动态。 ¿ 滞育如何改变 C.pipiens 的糖代谢？ ¿ C.pipiens 滞育期间储备的命运如何？