Regulation of Energy Metabolism in Insects

描述（由申请人提供）：Ae到目前为止获得的结果。埃及伊蚊代表了确定蚊子膳食蛋白中氨基酸命运的第一个动态方法。利用上次拨款期间开发的方法，我们现在可以更详细地探索氨基酸代谢的代谢控制，并开始分析幼虫发育过程中的代谢燃料增加，以及将这些方法应用于其他蚊子，如库蚊。这个项目的最终目标是在整个蚊子的背景下定义血液中蛋白质氨基酸的代谢，并从定量的角度理解不同的代谢通量是如何同时支持一次血食后的淋养循环和雌蚊子的生存的，因为除非她在第一次淋养循环中存活下来，否则她就没有机会再吃一次血并传播疾病。这样的分析为确定新的和意想不到的蚊虫控制目标提供了一个独特的机会。为下一个资助期提出以下具体目标：目标1：研究Ae的血粕氨基酸代谢命运。蚊雌性。蚊子是如何选择性地改变不同氨基酸的代谢命运的？在血粉消化过程中，主要的血淋巴氨基酸脯氨酸的代谢命运是什么？氨基酸氧化释放的氨代谢在淋养循环中是如何变化的？目的2：建立准确描述Ae代谢通量的定量模型。蚊雌性。什么模型最能描述淋养循环中氨基酸代谢的时间过程？如何将类似的定量模型应用于理解整个蚊子的代谢？目的3：研究Ae发育过程中代谢通量的动态变化。蚊。如何整合幼虫的新陈代谢以保证能量储备的合成？目的4确定Ae特定组织中发生的代谢变化。蚊雌性。不同代谢条件下蛋白质周转的程度如何？在淋养循环中，脂肪体和中肠的代谢是如何变化的？目的5：研究库蚊滞育过程中代谢通量的动态变化。滞育如何改变库蚊的糖代谢？储存量在库蚊滞育期间的命运如何？

DESCRIPTION (provided by applicant): The results obtained so far with Ae. aegypti represent the first dynamic approach to determining the fate of amino acids from meal proteins in mosquitoes. Using the methods developed during the last grant period, we are now in a position to probe the metabolic control of amino acid metabolism in more detail and to begin an analysis of metabolic fuel accretion during larval development, as well as, applying these methods to other mosquitoes, such as Culex pipiens. The ultimate aim of this project is to define blood meal protein amino acid metabolism in the context of the whole mosquito and to understand, in quantitative terms, how the different metabolic fluxes simultaneously support the gonotrophic cycle after a blood meal and the survival of the female mosquito, because unless she survives the first gonotrophic cycle there would be no opportunity to take a second blood meal and transmit disease. Such an analysis offers a unique opportunity to identify new and unexpected targets for mosquito control. The following specific aims are proposed for the next grant period: Aim 1: Investigate the metabolic fates of blood meal amino acids in Ae. aegypti females. How is the mosquito able to selectively alter the metabolic fate of different amino acids? What are the metabolic fates of proline, the major hemolymph amino acid, during blood meal digestion? How does the metabolism of ammonia released from amino acid oxidation change during a gonotrophic cycle? Aim 2: Develop quantitative models that accurately describe metabolic flux in Ae. aegypti females. What model best describes the time course for amino acid metabolism during the gonotrophic cycle? How can similar quantitative models be applied to understanding whole mosquito metabolism? Aim 3: Investigate the dynamics of metabolic flux during development in Ae. aegypti. How is the metabolism of larvae integrated to ensure the synthesis of energy reserves? Aim 4 Determine metabolic changes that occur in specific tissues of Ae. aegypti females. What is the extent of protein turnover under different metabolic conditions? How does the metabolism of the fat body and midgut change during the gonotrophic cycle? Aim 5: Investigate the dynamics of metabolic flux during diapause in C. pipiens. How does diapause alter sugar metabolism in C. pipiens? What is the fate of reserves during diapause in C. pipiens?