Microsphere Coatings for Immunoassays

描述（由申请人提供）：该项目旨在开发和展示一种简便、经济、广泛适用于诊断、生物技术和治疗用途的微/纳米颗粒薄膜涂层技术。这种方法需要制备和使用两亲嵌段共聚物，在疏水嵌段中含有光活化偶联和聚合基团，在亲水嵌段中含有钝化和生物分子偶联基团。目前的商业微颗粒缺乏易于提供流动颗粒和固定化微球阵列所需的表面特性，用于基因组，蛋白质组学和细胞组学分析系统；涂层纳米纤维在细胞和组织工程中的应用；包覆的“量子点”用于自编码粒子制备和需要活细胞摄取粒子的疾病研究和治疗。在第一阶段，将开发新的PhotoLink试剂和涂层技术，用于自组装单层膜在微纳米粒子和纳米粒子上的共价键，用于体外和体内诊断。预计在生物分子固定效率、稳定性和比活性方面，比目前的商业微球产品至少有50%的改进。该方案提出了两亲性嵌段共聚物设计的具有光活化双自由基固定和交联化学的自组装单层涂层，亲水性“刷”钝化涂层以及生物分子固定和稳定性能的创新组合。

DESCRIPTION (provided by applicant): This project is designed to develop and demonstrate a facile, cost-effective, and broadly applicable thin-film coating technology for micro/nanoparticles in diagnostic, biotech, and therapeutic uses. This approach requires the preparation and use of amphiphilic block copolymers containing photoactivatable coupling and polymerizing groups in the hydrophobic block and passivating and biomolecule coupling groups in the hydrophilic block. Current commercial microparticles lack the facile provision of surface properties needed for flowing particle and immobilized microsphere arrays for genomic, proteomic and cellomic assay systems; coated nanofibers for cellomic and tissue engineering applications; coated "quantum dots" for self-encoded particle preparation and for disease studies and treatment requiring particle uptake by living cells. In Phase I, new PhotoLink reagents and coating technology will be developed for covalent bonding of self-assembling monolayer film on micro- and nanoparticles for in vitro and in vivo diagnostic uses. A 50% minimum improvement over current commercial microsphere products is expected in the properties of biomolecule immobilization efficiency, stability, and specific activity. This plan presents an innovative combination in amphiphilic block copolymer design of self-assembling monolayer coating with photoactivatable diradical immobilization and crosslinking chemistry, hydrophilic "brush" passivating coating, and biomolecule immobilization and stabilization properties.