Microsphere Coatings for Immunoassays

用于免疫分析的微球涂层

• 批准号: 6994579
• 负责人: Kristin S Taton
• 金额: $ 9.99万
• 依托单位: INNOVATIVE SURFACE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994579
• 关键词: amphiphilicity; biomaterial development /preparation; copolymer; hydropathy; immunologic assay /test; microcapsule; molecular assembly /self assembly; photoactivation; photochemistry; polystyrenes; surface coating

DESCRIPTION (provided by applicant): This project is designed to develop and demonstrate a facile, cost-effective, and broadly applicable thin-film coating technology for micro/nanoparticles in diagnostic, biotech, and therapeutic uses. This approach requires the preparation and use of amphiphilic block copolymers containing photoactivatable coupling and polymerizing groups in the hydrophobic block and passivating and biomolecule coupling groups in the hydrophilic block. Current commercial microparticles lack the facile provision of surface properties needed for flowing particle and immobilized microsphere arrays for genomic, proteomic and cellomic assay systems; coated nanofibers for cellomic and tissue engineering applications; coated "quantum dots" for self-encoded particle preparation and for disease studies and treatment requiring particle uptake by living cells. In Phase I, new PhotoLink reagents and coating technology will be developed for covalent bonding of self-assembling monolayer film on micro- and nanoparticles for in vitro and in vivo diagnostic uses. A 50% minimum improvement over current commercial microsphere products is expected in the properties of biomolecule immobilization efficiency, stability, and specific activity. This plan presents an innovative combination in amphiphilic block copolymer design of self-assembling monolayer coating with photoactivatable diradical immobilization and crosslinking chemistry, hydrophilic "brush" passivating coating, and biomolecule immobilization and stabilization properties.

描述（由申请人提供）：该项目旨在开发和展示一种简便、经济高效且广泛适用的薄膜涂层技术，用于诊断、生物技术和治疗用途的微米/纳米颗粒。该方法需要制备和使用在疏水性嵌段中含有可光活化偶联和聚合基团以及在亲水性嵌段中含有钝化和生物分子偶联基团的两亲性嵌段共聚物。目前的商业微粒缺乏流动颗粒和固定微球阵列所需的表面特性，用于基因组、蛋白质组和细胞组测定系统；用于细胞组学和组织工程应用的涂层纳米纤维；涂层“量子点”，用于自编码粒子制备以及需要活细胞吸收粒子的疾病研究和治疗。在第一阶段，将开发新的 PhotoLink 试剂和涂层技术，用于将自组装单层膜共价键合在微米和纳米粒子上，用于体外和体内诊断用途。预计生物分子固定效率、稳定性和比活性性能将比现有商业微球产品至少提高 50%。该计划提出了自组装单层涂层与光活化双自由基固定和交联化学、亲水“刷”钝化涂层以及生物分子固定和稳定特性的两亲性嵌段共聚物设计的创新组合。