Multianalyte Nanomagnetic Biosensor Array for Pathogen Detection

描述（由申请人提供）：该SBIR第I阶段项目旨在将巨磁阻（GMR）传感器设备适配为用于病原体检测的“自旋电子”多分析物生物传感器。生物传感器技术的潜在价值正在被广泛应用，从医疗诊断到打击生物恐怖主义。该项目将侧重于开发GMR免疫测定法，以检测供水中的常见细菌和原生动物。磁性纳米/微米颗粒作为生物分子结合的标记物具有优势，包括稳定性和低背景信号。该项目将为NVE公司开发的GMR微电极阵列和海军研究实验室开发的用于珠阵列计数器（BARC）生物传感器系统的顺磁性微珠的生物传感器应用提供改进的生物分子偶联技术。第一阶段的工作将包括合成和使用测试的光化学和电化学聚合物衍生物结合单克隆和多克隆抗体的氮化硅表面和磁性颗粒分别。将通过荧光和磁性测定方法测量信号/噪声特性，并显示其等于或上级当前使用的测定方法。这些阶段I结果预期提供用于优化阶段II中的生物分子固定化化学的坚实技术背景，以及用于增强GMR生物传感器流动池的微流体通道涂层和盖粘附的新的但相关的潜在反应性聚合物。开发一种功能性的低成本、便携式的病原体GMR生物传感器将有助于人类健康，因为它允许在现场快速分析当地的供水，从而最大限度地减少肠道感染。

DESCRIPTION (provided by applicant): This SBIR Phase I project is designed to adapt a Giant Magneto-Resistive (GMR) sensor device into a "spintronic" multianalyte biosensor for pathogen detection. The potential value of biosensor technology is being recognized for a wide variety of applications, from medical diagnostics to countering bioterrorism. This project will focus on developing GMR immunoassays to detect common bacteria and protozoa in water supplies. Magnetic nano/microparticles present advantages as labels for biomolecule binding, including stability and low background signal. This project will provide improved biomolecule coupling technology for biosensor applications of the GMR microelectrode array under development by NVE Corp. and the paramagnetic microbeads under development as labels by the Naval Research Laboratories for the Bead Array Counter (BARC) biosensor system. The Phase I effort will include the synthesis and use testing of photochemical and electrochemical polymer derivatives for binding monoclonal and polyclonal antibodies to the silicon nitride surface and magnetic particles respectively. Signal/noise properties will be measured by fluorescent and magnetic assay methods and shown to be equal or superior to currently used assays. These Phase I results are expected to provide a solid technical background for optimizing the biomolecule immobilization chemistry in Phase II, as well as new, but related latent-reactive polymers for enhanced microfluidic channel coating and lid adhesion for the GMR biosensor flow cell. Development of a functional low cost, portable GMR biosensor for pathogens will aid human health by allowing quick analysis of local water supplies in the field, thereby minimizing diarrheal infections.