Malaria Parasite Receptors And Host Cell Ligands

目的：1.鉴定疟原虫红细胞侵入受体和感染红细胞与内皮细胞和胎盘黏附的受体。2.确定每个寄生虫受体的红细胞配体。3.确定变异抗原中与胎盘结合的最小结构域。4.在变异抗原上测试一个区域，以诱导对恶性疟原虫的保护和克服变异。 1.BAEBL配体可被纯化的血糖蛋白C阻断，但不能被N-葡聚糖酶处理的血糖蛋白C所阻断。与沙鼠红细胞相比，正常红细胞的血糖蛋白C上的N-糖链有所不同。2.鉴定了AMA1与红细胞结合的受体结构域以及与AMA1结合的红细胞上的Kx蛋白。3.确定了DD2(神经氨酸酶处理的红细胞无侵袭)和DD2/nm(神经氨酸酶处理的红细胞侵袭)之间的差异。

Purpose: 1. To identify the receptors on malaria parasites for red cell invasion and on infected red cells for adhesion to endothelium and to placenta. 2. To identify the red cell ligands for each parasite receptor. 3. To identify the minimal domain in the variant antigen for binding to placenta. 4. Test a region on the variant antigen for inducing protection against P. falciparum in Aotus monkeys and for overcoming variation. Accomplishments during the year: 1. The BAEBL ligand is blocked by purified glycophorin C, but not by N-glycanase treated glycophorin C. The N-glycan on glycophorin C differs for normal red cells compared to Gerbich red cells 2. The receptor domain of AMA1 for binding red cells has been identified as well as the Kx protein on the red cell for binding of AMA1. 3. The difference between Dd2 (no invasion of neuraminidase treated red cells) and Dd2/nm (invasion of neuraminidase treated red cells) has been identified.