Infrastructure for Human Embryonic Stem Cells Productio*

描述（由申请人提供）：本申请的目的是使NIH人类ES细胞注册表中列出的人类ES细胞系可供全球研究界使用。 我们的目标是在第一年能够分配200等份的人类ES细胞（每份至少含有2x10（6）个细胞），并在第二年将生产和分配率提高一倍。 我们还将为接受者提供培训，其中包括：实验室手册，实践培训和网站支持。 为了确保在严格的质量控制过程（良好实验室规范）下扩大高质量的人类ES细胞，我们希望建立一个新的设备齐全的实验室，并增加训练有素的生物学家的数量。该团队还将参与旨在改善人类ES细胞的基础研究。探讨人胚胎干细胞系在生长条件和分化能力方面的变化。 这将显著增加人类ES细胞的研究应用潜力，以及发现用于治疗一些最常见和最具破坏性的人类疾病的潜在人类ES细胞衍生疗法的速度。

DESCRIPTION (provided by applicant): The aim of this application is to make the lines of human ES cells listed on the NIH Human ES cells registry available to the research community worldwide. We aim to be able to distribute 200 aliquots of human ES cells (each containing at least 2x10(6) cell) during the first year, and to double the production and distribution rate during the second year. We will also provide training to recipients which will include: lab manual, hands-on training and a web site support. To ensure the scale-up of high quality human ES cells under tight quality control processes (Good Laboratory Practice), we wish to establish a new fully-equipped laboratory and increase the number of the well-trained biologists. The team will also be involved in basic research aiming at improving human ES-cells. And to explore the variation in growth requirements and differentiation capacity of human ES cell lines. This will significantly increase the potential of research applications of human ES cells and the rate of discoveries of potential human ES cell-derived therapies for treating some of the most common and devastating human diseases.