Short-term course in human embryonic stem cell culture *

人类胚胎干细胞培养短期课程*

• 批准号: 6911613
• 负责人: JOSEPH ITSKOVITZ-ELDOR
• 金额: $ 15.85万
• 依托单位: TECHNION-ISRAEL INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6911613
• 关键词: Short; term; course; human; embryonic

DESCRIPTION (provided by applicant): Human embryonic stem (hES) cell lines offer a unique new research tool with widespread possible clinical application. Great effort is invested throughout the world in order to encourage the large scale growth and distribution of these cells. The main aim of this proposal is to follow the hES cell distribution carried out under NIH grant PAR-02-023 (" Human Embryonic Stem Cell Resource Infrastructure Enhancement") with short-term courses in hES cell culturing techniques. The courses are designed to ensure a successful establishment of hES cell colony at the graduates' laboratories. The courses will take place in Israel and in the United States with the assistance of the staff of Stem Cell Biology Unit/Laboratory of Neuroscience/National Institute on Aging/NIH. In order to address the various individual requirements of each of the participants, we aim to offer two alternative courses: * An introductory course designed for training basic hES cells culturing techniques. * An advanced course including basic and advanced techniques of hES cells, including animal-free culture system, principle in vitro and in vivo differentiation methods, sorting and genetic manipulation techniques. A periodical follow-up communication with each course graduate will be initiated to provide further support and guidance. Thus course graduates will enjoy close feed-back when establishing the colonies while instructors will have the opportunity to estimate the success rate of the course. Taken together, this guiding program will encourage the distribution and research based on hES cells.

描述(申请人提供)：人类胚胎干细胞(HES)细胞系提供了一种独特的新研究工具，具有广泛的临床应用前景。全世界都投入了巨大的努力，以鼓励这些细胞的大规模生长和分布。这项建议的主要目的是跟踪在NIH资助PAR-02-023(“人类胚胎干细胞资源基础设施增强”)下进行的HES细胞分布，并提供HES细胞培养技术的短期课程。这些课程旨在确保在研究生实验室成功建立HES细胞克隆。这些课程将在以色列和美国进行，由干细胞生物学单位/神经科学实验室/国家老龄研究所/国立卫生研究院的工作人员提供协助。为了满足每个参与者的不同个人需求，我们计划提供两种不同的课程：*为培训基本的HES细胞培养技术而设计的入门课程。*一个高级课程，内容包括HES细胞的基本和高级技术，包括无动物培养系统、体外和体内分化原理、分选和遗传操作技术。将与每一位课程毕业生进行定期的后续沟通，以提供进一步的支持和指导。因此，课程毕业生在建立殖民地时将享受到密切的反馈，而教师将有机会估计课程的成功率。综上所述，该指导计划将鼓励基于HES细胞的分布和研究。