Short-term course in human embryonic stem cell culture *

描述（由申请人提供）：人类胚胎干（hES）细胞系提供了一种独特的新研究工具，具有广泛的临床应用可能。为了鼓励这些细胞的大规模生长和分布，全世界都投入了巨大的努力。该提案的主要目的是遵循NIH拨款PAR-02-023（“人类胚胎干细胞资源基础设施增强”）进行的he细胞分布，并提供he细胞培养技术的短期课程。这些课程旨在确保在毕业生的实验室中成功建立hES细胞群。这些课程将在干细胞生物学单位/神经科学实验室/国家老龄化研究所/美国国立卫生研究院的工作人员的协助下在以色列和美国进行。为了满足每个参与者的不同个人需求，我们的目标是提供两个替代课程：*一个入门课程，旨在培训基本的he细胞培养技术。*高级课程，包括he细胞的基础和高级技术，包括无动物培养系统、体外和体内分化方法原理、分选和基因操作技术。定期与各课程毕业生进行后续沟通，以提供进一步的支持和指导。因此，课程毕业生在建立殖民地时将享有密切的反馈，而教师将有机会估计课程的成功率。综上所述，该指导计划将鼓励基于he细胞的分布和研究。

DESCRIPTION (provided by applicant): Human embryonic stem (hES) cell lines offer a unique new research tool with widespread possible clinical application. Great effort is invested throughout the world in order to encourage the large scale growth and distribution of these cells. The main aim of this proposal is to follow the hES cell distribution carried out under NIH grant PAR-02-023 (" Human Embryonic Stem Cell Resource Infrastructure Enhancement") with short-term courses in hES cell culturing techniques. The courses are designed to ensure a successful establishment of hES cell colony at the graduates' laboratories. The courses will take place in Israel and in the United States with the assistance of the staff of Stem Cell Biology Unit/Laboratory of Neuroscience/National Institute on Aging/NIH. In order to address the various individual requirements of each of the participants, we aim to offer two alternative courses: * An introductory course designed for training basic hES cells culturing techniques. * An advanced course including basic and advanced techniques of hES cells, including animal-free culture system, principle in vitro and in vivo differentiation methods, sorting and genetic manipulation techniques. A periodical follow-up communication with each course graduate will be initiated to provide further support and guidance. Thus course graduates will enjoy close feed-back when establishing the colonies while instructors will have the opportunity to estimate the success rate of the course. Taken together, this guiding program will encourage the distribution and research based on hES cells.