Infrastructure for Human Embryonic Stem Cells Productio*

描述（由申请人提供）： 该提案的主要目的是促进用于人类胚胎干细胞（hESC）培养的基本方法，以鼓励其在饲养层，动物和无血清条件下的大规模生长及其分布。此外，我们的目标是进一步表征这些细胞，并根据良好实验室规范（GLP）法规培养它们。通过实现这些目标，我们将能够分发不仅适用于基础研究，而且有可能用于临床和工业用途的hESC。 为了实现这些目标，我们的研究团队将专注于以下方面：  探索hESC的最佳培养条件：饲养层，动物和无血清条件。  在GLP中生长和繁殖hESC。  生成用于评估细胞质量的一组测量结果。  在不同的培养条件下，全面表征四种Technion NIH注册细胞 培养条件下，在长期培养后和早期分化事件期间。  改进现有的冻融hESC的方法。 这些改进将构成在促进这些细胞的研究和临床利用的条件下大量生长和繁殖hESC的能力。

DESCRIPTION (provided by applicant): The main aim of this proposal is to promote the basic methodology used for human embryonic stem cells (hESCs) culturing in order to encourage their large-scale growth in feeder layer-, animal- and serum-free conditions, and their distributions. In addition, we aim to further characterize these cells and to culture them under good laboratory practices (GLP) regulations. By achieving these goals, we will be able to distribute hESCs that will not only be suitable for basic studies but also potentially for clinical and industrial uses. To meet these aims our research team will concentrate on the following;  Exploring the optimal culture conditions for hESCs: feeder layer-, animal- and serum-free conditions.  Growing and propagating hESCs in GLP.  Generating a set of measurements for the evaluation of the cells' quality.  Characterizing comprehensively the four Technion NIH-registered cells during their culture in various culture conditions, after prolonged culture and during early differentiation events.  Improving the existing methods for freezing and thawing hESCs. These improvements will constitute the ability to grow and propagate hESCs in large quantities in conditions that promote both research and clinical utilization of these cells.