Tools To Study Cell Motility and Ion Channels

研究细胞运动和离子通道的工具

• 批准号: 7010631
• 负责人: GEORGE M WHITESIDES
• 金额: $ 31.73万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010631
• 关键词: axon; bacteria; cell line; cell migration; cell motility; chemotaxis; electrophysiology; extracellular matrix; membrane channels; microfluidics; technology /technique development; tissue /cell culture; voltage /patch clamp

DESCRIPTION (provided by applicant): This research will study two areas: Mechanisms of cell motility, and molecular action of ion channels in cell membranes. It has three specific foci: i) the motility of mammalian cells; ii) the motility of bacterial cells; and iii) electrophysioiogy of ion channels. The work will also develop new experimental methods for examining these biological phenomena and processes. These developments will emphasize microtools, fabricated using a combination of soft lithography, self-assembled monolayers (SAMs), microfluidics, and electrochemistry. These projects are tied together by three common themes: motility, sensing, and new, microfabricated tools for biology/biochemistry. The work will have three broad outcomes. It will clarify the molecular and cell physiological mechanisms of motility in mammalian and bacterial cells. It will provide new systems for studying ion channels, it will develop new tools to support these studies, and demonstrate these tools in the context of biological problems. These studies are relevant to a number of areas of biology in which an understanding of the mechanisms of cellular motility and cellular sensing are important; from fundamental studies of development, metastasis, angiogenesis, and infectious disease to applications in cell-based sensors and tools for high-throughput screening of leads. The work spans biology, microfabrication, and biophysics, and an important product of the research will be students broadly trained in biological and physical sciences.

描述（由申请人提供）：本研究将研究两个领域：细胞运动机制和细胞膜离子通道的分子作用。它有三个特定的焦点：i)哺乳动物细胞的运动性；Ii)细菌细胞的运动性；离子通道的电生理。这项工作还将开发新的实验方法来检查这些生物现象和过程。这些发展将强调使用软光刻、自组装单层（sam）、微流体和电化学相结合的微工具。这些项目由三个共同的主题联系在一起：运动性、传感和用于生物/生物化学的新型微制造工具。这项工作将产生三大成果。这将阐明哺乳动物和细菌细胞运动的分子和细胞生理机制。它将为研究离子通道提供新的系统，它将开发新的工具来支持这些研究，并在生物学问题的背景下展示这些工具。这些研究与许多生物学领域相关，在这些领域中，对细胞运动和细胞感知机制的理解是重要的；从发展、转移、血管生成和传染病的基础研究到基于细胞的传感器和用于高通量筛选导联的工具的应用。这项工作涵盖了生物学、微加工和生物物理学，研究的一个重要成果将是培养学生在生物和物理科学方面的广泛训练。

项目成果

Fabrication of high-aspect-ratio metallic nanostructures using nanoskiving.

• DOI: 10.1021/nl0615672
• 发表时间: 2006-07
• 期刊: Nano letters
• 影响因子: 10.8
• 作者: Qiaobing Xu;R. Perez-Castillejos;Zhefeng Li;G. Whitesides

Surface plasmon resonances of free-standing gold nanowires fabricated by nanoskiving.

• DOI: 10.1002/anie.200600394
• 发表时间: 2006-05
• 期刊: Angewandte Chemie
• 作者: Qiaobing Xu;J. Bao;F. Capasso;G. Whitesides

Geometric confinement influences cellular mechanical properties II -- intracellular variances in polarized cells.

几何限制影响细胞机械特性 II——极化细胞的细胞内差异。

• 发表时间: 2007
• 期刊: Molecular & cellular biomechanics : MCB
• 作者: Su,Judith;Brau,RicardoR;Jiang,Xingyu;Whitesides,GeorgeM;Lange,MatthewJ;So,PeterTC
• 通讯作者: So,PeterTC

Rapid prototyping of microstructures by soft lithography for biotechnology.

• DOI: 10.1007/978-1-60327-106-6_3
• 发表时间: 2010
• 期刊: Methods in molecular biology
• 作者: D. Wolfe;D. Qin;G. Whitesides

Subnanometer replica molding of molecular steps on ionic crystals.

• DOI: 10.1021/nl102409d
• 发表时间: 2010-10-13
• 期刊: Nano letters
• 影响因子: 10.8
• 作者: Elhadj S;Rioux RM;Dickey MD;DeYoreo JJ;Whitesides GM
• 通讯作者: Whitesides GM