Rapid Prototyping of 3D Nanofluidic Systems in Glass Substrates

玻璃基板中 3D 纳米流体系统的快速原型制作

• 批准号: 7077942
• 负责人: ALAN J HUNT
• 金额: $ 22.91万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7077942
• 关键词: density; drug delivery systems; lasers; microfluidics

DESCRIPTION (provided by applicant): We recently developed a method for nanomachining in hard materials using a femtosecond pulsed laser (Joglekar et al., 2003, 2004), which we have recently extended to the rapid production of three-dimensional (3D) subsurface nanofluidic and microfluidic networks in glass substrates. The overall aim of this proposal is to apply this unique capability to fabricate extremely high density and increased functionality micro- and nanofluidic devices for biomedical diagnostics and biochemical analysis. To our knowledge, this is the first time that three-dimensional complexity, with precision on the order of ten nanometers, has been available for glass microfluidics. We hypothesize that a variety of novel bioanalysis and drug-delivery systems may be fabricated for applications in medical diagnostics, purification, sequencing, and proteomic analysis. The overall aim of this R21 proposal is to aggressively explore the limits of this nanomachining technique, methodically explore transport of biomolecules within nanochannels constructed in this manner, and develop at least one highly integrated system with compelling characterization data. The proposed plan of research is unique and potentially of very high impact in that it enables entirely new classes of micro- and nanofluidic devices that are no longer limited to either two-dimensional geometries or multiple layers of polymer materials. Specific Aims are 1: Determine the physical and practical limits of 3-D femtosecond machining for micro- and nanofluidics applications 2: Thoroughly explore flow and transport in 3D femtosecond laser-machined micro- and nanochannels 3: Employ 3D nanomachining to rapidly prototype high-density flow control components and integrated analysis devices. The overall goal of this aim is to create a compelling demonstration of the use of a flow- control toolbox for microfluidics in glass substrates that could be used for microarray, microseparations, or micro-drug delivery systems.

描述（由申请人提供）：我们最近开发了一种使用飞秒脉冲激光在硬质材料中进行纳米加工的方法（Joglekar等人，2003，2004），我们最近扩展到快速生产的三维（3D）的表面下的纳米流体和微流体网络在玻璃基板。该提案的总体目标是应用这种独特的能力来制造用于生物医学诊断和生化分析的极高密度和增加功能的微流体和纳米流体设备。据我们所知，这是第一次三维复杂性，精度在10纳米量级，已可用于玻璃微流体。我们假设，各种新型的生物分析和药物输送系统可以制造用于医学诊断，纯化，测序和蛋白质组学分析。这个R21提案的总体目标是积极探索这种纳米加工技术的局限性，有条不紊地探索以这种方式构建的纳米通道内生物分子的运输，并开发至少一个具有令人信服的表征数据的高度集成系统。拟议的研究计划是独特的，并且可能具有非常高的影响力，因为它使全新的微流体和纳米流体设备不再限于二维几何形状或多层聚合物材料。具体目标为1：确定三维飞秒激光加工在微纳流体应用中的物理和实际限制2：彻底探索三维飞秒激光加工的微纳通道中的流动和传输3：采用三维纳米加工快速制作高密度流量控制组件和集成分析设备的原型。这个目标的总体目标是创建一个令人信服的演示，使用一个流动控制工具箱的微流体在玻璃基板，可用于微阵列，微分离，或微药物输送系统。