权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Nanoscale patterning that promotes cell adhesion

促进细胞粘附的纳米级图案

基本信息

批准号：
7140654
负责人：
Heather D Maynard
金额：
$ 18.28万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-23 至 2008-08-31
项目状态：
已结题

项目摘要

Cell adhesion is governed by interactions of cell surface receptors with proteins found inthe extracellular matrix (ECM). Nanometer and micrometer length scales are relevant in this process, and flexible strategies to pattern cell adhesion ligands derived from ECM proteins, particularly at the micro- and nanoscale, provides tremendous opportunities to study and control cell behavior. One objective of this research is to employ ultraviolet (UV) irradiation to selectively chemically transform programmable polymer surfaces to micropattern cell adhesion peptides. A second objective is to translate the technology using low intensity electron beam (e-beam) radiation to fabricate nanopatterns of peptides. We hypothesize that micropatterns and nanopatterns of cell adhesion peptides fabricated using UV or e-beam radiation and a pH- sensitive polymer surface will promote cell adhesion. We have proposed two specific aims to reach our objectives. The first aim is to quantify osteoblast behavior on surfaces micropatterned with cell adhesion peptides. Polymer surfaces will be prepared and subsequently converted to micropatterns of amine-reactive groups using UV light, a photoacid generator (PAG), and a mask. Cell adhesion peptides will be conjugated to the surfaces. In vitro cell culture will verify that the surfaces promote osteoblast adhesion, proliferation, and differentiation. The second aim is to fabricate nanoarrays of biomolecules using pH sensitive surfaces. Nanopatterns of biomolecules will be generated using e-beam radiation. Osteoblast adhesion will be demonstrated to validate the approach. One potential outcome of this research is the development of new implant coatings that promote osseointegration. The second potential outcome is a general strategy to pattern biomolecules with nanometer resolution to study cell adhesion. The long-term goal of this research is to employ a flexible patterning technique to determine the critical sizes, shapes, and physical separations of cell adhesion ligands at the nanoscale. Such information is essential to the rational design of biomaterials and to understanding the mechanisms by which signals from the ECM are transduced to the cell interior. Relevance. Surface coatings that promote and control cell behavior can lead to better human implants and devices.

细胞粘附是由细胞表面受体与细胞外基质（ECM）中的蛋白质相互作用所控制的。纳米和微米长度尺度在这一过程中是相关的，而从ECM蛋白衍生的细胞粘附配体的灵活策略，特别是在微纳米尺度上，为研究和控制细胞行为提供了巨大的机会。本研究的目的之一是利用紫外线（UV）照射选择性地将可编程聚合物表面化学转化为微图案细胞粘附肽。第二个目标是利用低强度电子束（电子束）辐射来制造肽的纳米图案。我们假设使用紫外线或电子束辐射和pH敏感聚合物表面制备的细胞粘附肽的微模式和纳米模式将促进细胞粘附。为实现我们的目标，我们提出了两个具体目标。第一个目标是量化成骨细胞在细胞粘附肽微图案化表面上的行为。聚合物表面将被制备并随后转化为胺反应性的微模式