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Nanoscale patterning that promotes cell adhesion

促进细胞粘附的纳米级图案

基本信息

批准号：
7140654
负责人：
Heather D Maynard
金额：
$ 18.28万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-23 至 2008-08-31
项目状态：
已结题

项目摘要

Cell adhesion is governed by interactions of cell surface receptors with proteins found inthe extracellular matrix (ECM). Nanometer and micrometer length scales are relevant in this process, and flexible strategies to pattern cell adhesion ligands derived from ECM proteins, particularly at the micro- and nanoscale, provides tremendous opportunities to study and control cell behavior. One objective of this research is to employ ultraviolet (UV) irradiation to selectively chemically transform programmable polymer surfaces to micropattern cell adhesion peptides. A second objective is to translate the technology using low intensity electron beam (e-beam) radiation to fabricate nanopatterns of peptides. We hypothesize that micropatterns and nanopatterns of cell adhesion peptides fabricated using UV or e-beam radiation and a pH- sensitive polymer surface will promote cell adhesion. We have proposed two specific aims to reach our objectives. The first aim is to quantify osteoblast behavior on surfaces micropatterned with cell adhesion peptides. Polymer surfaces will be prepared and subsequently converted to micropatterns of amine-reactive groups using UV light, a photoacid generator (PAG), and a mask. Cell adhesion peptides will be conjugated to the surfaces. In vitro cell culture will verify that the surfaces promote osteoblast adhesion, proliferation, and differentiation. The second aim is to fabricate nanoarrays of biomolecules using pH sensitive surfaces. Nanopatterns of biomolecules will be generated using e-beam radiation. Osteoblast adhesion will be demonstrated to validate the approach. One potential outcome of this research is the development of new implant coatings that promote osseointegration. The second potential outcome is a general strategy to pattern biomolecules with nanometer resolution to study cell adhesion. The long-term goal of this research is to employ a flexible patterning technique to determine the critical sizes, shapes, and physical separations of cell adhesion ligands at the nanoscale. Such information is essential to the rational design of biomaterials and to understanding the mechanisms by which signals from the ECM are transduced to the cell interior. Relevance. Surface coatings that promote and control cell behavior can lead to better human implants and devices.

细胞粘附受细胞表面受体与细胞外基质（ECM）中蛋白质相互作用的控制。纳米和微米的长度尺度是相关的，在这个过程中，和灵活的策略，图案细胞粘附配体衍生自ECM蛋白质，特别是在微米和纳米级，提供了巨大的机会，研究和控制细胞的行为。本研究的一个目的是采用紫外线（UV）照射选择性地化学转化可编程聚合物表面的微图案细胞粘附肽。第二个目标是使用低强度电子束（e-束）辐射来转化该技术以制造肽的纳米片。我们假设使用UV或电子束辐射和pH敏感的聚合物表面制造的细胞粘附肽的微图案和纳米图案将促进细胞粘附。我们提出了两个具体目标，以实现我们的目标。第一个目的是量化成骨细胞的行为与细胞粘附肽微图案化的表面。聚合物表面将被制备并随后转化为胺反应性微图案。使用UV光、光酸产生剂（PAG）和掩模的基团。细胞粘附肽将与表面缀合。体外细胞培养将验证表面促进成骨细胞粘附、增殖和分化。第二个目标是使用pH敏感表面制造生物分子的纳米阵列。生物分子的纳米粒子将使用电子束辐射产生。将证明成骨细胞粘附以验证该方法。这项研究的一个潜在成果是开发新的种植体涂层，促进骨整合。第二个可能的结果是一项总体战略，以纳米分辨率对生物分子进行图案化，以研究细胞粘附。这项研究的长期目标是采用灵活的图案化技术，以确定在纳米尺度上的细胞粘附配体的临界尺寸，形状和物理分离。这些信息对于生物材料的合理设计和理解ECM信号转导到细胞内部的机制至关重要。本案无关促进和控制细胞行为的表面涂层可以导致更好的人体植入物和设备。