Nanoscale patterning that promotes cell adhesion

促进细胞粘附的纳米级图案

基本信息

批准号：
7140654
负责人：
Heather D Maynard
金额：
$ 18.28万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-23 至 2008-08-31
项目状态：
已结题

项目摘要

Cell adhesion is governed by interactions of cell surface receptors with proteins found inthe extracellular matrix (ECM). Nanometer and micrometer length scales are relevant in this process, and flexible strategies to pattern cell adhesion ligands derived from ECM proteins, particularly at the micro- and nanoscale, provides tremendous opportunities to study and control cell behavior. One objective of this research is to employ ultraviolet (UV) irradiation to selectively chemically transform programmable polymer surfaces to micropattern cell adhesion peptides. A second objective is to translate the technology using low intensity electron beam (e-beam) radiation to fabricate nanopatterns of peptides. We hypothesize that micropatterns and nanopatterns of cell adhesion peptides fabricated using UV or e-beam radiation and a pH- sensitive polymer surface will promote cell adhesion. We have proposed two specific aims to reach our objectives. The first aim is to quantify osteoblast behavior on surfaces micropatterned with cell adhesion peptides. Polymer surfaces will be prepared and subsequently converted to micropatterns of amine-reactive groups using UV light, a photoacid generator (PAG), and a mask. Cell adhesion peptides will be conjugated to the surfaces. In vitro cell culture will verify that the surfaces promote osteoblast adhesion, proliferation, and differentiation. The second aim is to fabricate nanoarrays of biomolecules using pH sensitive surfaces. Nanopatterns of biomolecules will be generated using e-beam radiation. Osteoblast adhesion will be demonstrated to validate the approach. One potential outcome of this research is the development of new implant coatings that promote osseointegration. The second potential outcome is a general strategy to pattern biomolecules with nanometer resolution to study cell adhesion. The long-term goal of this research is to employ a flexible patterning technique to determine the critical sizes, shapes, and physical separations of cell adhesion ligands at the nanoscale. Such information is essential to the rational design of biomaterials and to understanding the mechanisms by which signals from the ECM are transduced to the cell interior. Relevance. Surface coatings that promote and control cell behavior can lead to better human implants and devices.

细胞粘附受细胞表面受体与细胞外基质（ECM）中发现的蛋白质的相互作用。纳米长度和微米长度尺度在此过程中是相关的，并且具有源自ECM蛋白的细胞粘附配体的灵活策略，尤其是在微观和纳米级时，为研究和控制细胞行为提供了巨大的机会。这项研究的一个目的是利用紫外线（UV）辐射来选择性化学化学转化可编程的聚合物表面，向微孔图案细胞粘附肽。第二个目标是使用低强度电子束（E-BEAM）辐射转换技术，以制造肽的纳米图案。我们假设使用紫外线或电子束辐射制造的细胞粘附肽和pH敏感聚合物表面的细胞粘附肽的微图和纳米图质将促进细胞粘附。我们提出了两个具体目标以实现我们的目标。第一个目的是量化用细胞粘附肽微图案的表面上的成骨细胞行为。聚合物表面将准备并随后转化为胺反应的微图案使用紫外线，光酸发生器（PAG）和面具的组。细胞粘附肽将偶联到表面。体外细胞培养将验证表面是否促进成骨细胞粘附，增殖和分化。第二个目的是使用pH敏感表面制造生物分子的纳米阵列。生物分子的纳米图案将使用电子束辐射产生。将证明成骨细胞粘附以验证该方法。这项研究的潜在结果是开发促进骨整合的新植入涂层。第二个潜在结果是一般策略具有纳米分辨率的模式生物分子研究细胞粘附。这项研究的长期目标是采用灵活的图案技术来确定纳米级细胞粘附配体的临界大小，形状和物理分离。此类信息对于生物材料的合理设计至关重要，并了解将ECM信号传递到细胞内部的机制。关联。促进和控制细胞行为的表面涂层会导致更好的人植入物和设备。