Project to be confirmed after rotation year

突触前囊泡释放的神经递质使神经元在大脑中进行交流。然而，突触内不同功能类型的突触囊泡的详细空间排列仍不清楚.我们将使用最先进的超分辨率显微镜，包括多色，实时和3DSTORM/STED显微镜，以及扩展显微镜，以及开发新的计算图像分析方法来解决这个问题。然后，我们将使用这些方法来了解在突触发育过程中，这种变化是如何对神经元活动做出反应的，以及当关键的突触蛋白受到干扰时。

Neurotransmitter release from presynaptic vesicles at the synapse allows neurons tocommunicate in the brain. However the detailed spatial arrangement of differentfunctional types of synaptic vesicles within the synapse remains unknown. We will usestate-of-the-art super-resolution microscopy, including multi-colour, live and 3DSTORM/STED microscopy, and expansion microscopy, together with developing newcomputational image analysis approaches, to address this question. We will then usethese approaches to understand how this changes during synapse development, inresponse to neuronal activity and when key synaptic proteins are perturbed.