COBRE: Membrane Protein Production and Characterization

COBRE：膜蛋白生产和表征

• 批准号: 6988625
• 负责人: ABRAHAM M LENHOFF
• 金额: $ 173.62万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6988625
• 关键词: membrane proteins; protein structure function

DESCRIPTION (provided by applicant): The COBRE on Membrane Protein Production and Characterization (COMPPAC) is motivated by the disparity between the critical role of membrane proteins in biology and the well-recognized deficiencies in production and structural characterization of these molecules. We will address this challenge via a suite of five subprojects involving seven PIs from four academic disciplines at all faculty ranks, with all five of the lead PIs being either early-career or junior investigators. The scope of the research spans a spectrum from membrane protein overexpression, solubilization and purification, to structure-function studies based on crystallographic characterization, to predominantly functional studies of biomedically significant or related membrane proteins. The membrane proteins to be studied span a variety of structural and functional characteristics, including receptors, adhesion proteins and interfacial enzymes, and their relevance to several biomedically important conditions will be examined, including cancer, spermatogenesis and chemical warfare. The COBRE will have a strong interdisciplinary flavor by virtue of collaborations within and between subprojects, and as the initial subprojects acquire independent support, the scope of the research activities will be expanded by complementary research within several additional active membrane protein research labs. Core facilities support to be provided for protein production, structural biology and bioimaging is closely integrated with the substantial infrastructure for biomolecular research at the University of Delaware and the Delaware Biotechnology Institute. The COBRE will also foster the systematic application of the methods under development to membrane proteins in general, especially for the expression, purification and structural biology studies. This can have a significant impact on the membrane protein research community at large, and on the national infrastructure on membrane protein structural biology being developed within the NIH Roadmap.

描述(由申请人提供)： 关于膜蛋白生产和表征的COMPPAC(COMPPAC)是由膜蛋白在生物学中的关键作用与公认的这些分子在生产和结构表征方面的缺陷之间的差异所推动的。我们将通过一套五个子项目来应对这一挑战，涉及来自四个学科的所有教职级的七名PI，所有五名首席PI要么是职业生涯早期的调查人员，要么是初级调查人员。研究范围从膜蛋白的过度表达、增溶和纯化，到基于结晶学特征的结构-功能研究，再到具有生物医学意义的或相关的膜蛋白的主要功能研究。将研究的膜蛋白跨越各种结构和功能特征，包括受体、黏附蛋白和界面酶，并将研究它们与几种生物医学重要疾病的相关性，包括癌症、精子发生和化学战。COBRE将凭借子项目内部和子项目之间的合作而具有强烈的跨学科色彩，随着初始子项目获得独立支持，研究活动的范围将通过在多个活跃的膜蛋白研究实验室内进行补充研究来扩大。为蛋白质生产、结构生物学和生物成像提供的核心设施支持与特拉华大学和特拉华生物技术研究所的生物分子研究的实质性基础设施紧密结合在一起。Cobre还将促进正在开发的方法对膜蛋白的系统应用，特别是在表达、纯化和结构生物学研究方面。这可能会对整个膜蛋白研究界产生重大影响，并对NIH路线图内正在制定的膜蛋白结构生物学的国家基础设施产生重大影响。