LASER SCANNING CYTOMETER

激光扫描细胞仪

基本信息

批准号：
6878180
负责人：
Anna Huttenlocher
金额：
$ 38.12万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-15 至 2006-09-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6878180
关键词：
biomedical equipment purchase flow cytometry

项目摘要

DESCRIPTION (provided by applicant): This application requests funding for a CompuCyte laser scanning cytometer optimized for quantitative analysis of fluorescence in fixed and living cells and tissues. The laser scanning cytometer will become part of the University of Wisconsin Comprehensive Cancer Center's flow cytometry facility, and will serve the entire University of Wisconsin research community. The facility will serve investigators from multiple departments throughout the University of Wisconsin campus who need the instrument to quantify fluorescence in homogenous and heterogeneous cell populations with both live and fixed samples. The major users will require the CompuCyte laser scanning cytometer to (1) provide quantification of fluorescence in adherent cells and (2) analyze heterogeneous populations of cells within mixed populations or tissues. All major users have experience in fluorescence microscopy and flow cytometry analysis and have determined that the equipment currently available at the University of Wisconsin cannot meet their needs. The laser scanning cytometer will be installed in a flow cytometry facility laboratory located near the laboratory of the PI at a location central to the other investigators. Personnel experienced in flow cytometry will staff the facility and financial support for the facility will be provided by the University of Wisconsin Comprehensive Cancer Center. In summary, the application will serve the needs of a broad NIH-funded scientific community at the University of Wisconsin campus and will provide a significant advance over the current technology available for fluorescence analysis.

描述（由申请人提供）：此申请要求对合并激光扫描细胞仪进行资金，以优化用于定量固定和活细胞和组织中荧光的定量分析。激光扫描细胞仪将成为威斯康星大学综合癌症中心流式细胞仪设施的一部分，并将为整个威斯康星大学研究社区服务。该设施将为威斯康星大学校园多个系的研究人员提供服务，他们需要该工具来量化具有活样品和固定样品的同质和异质细胞种群中的荧光。主要用户将要求compucyte激光扫描细胞仪至（1）提供粘附细胞中荧光的定量，（2）分析混合种群或组织中细胞的异质种群。所有主要用户都有荧光显微镜和流式细胞仪分析的经验，并确定威斯康星大学目前可用的设备无法满足他们的需求。激光扫描细胞仪将安装在位于PI实验室附近的流式细胞仪设施实验室中，位于其他研究人员中心的位置。流式细胞仪经验丰富的人员将为该设施的设施和财政支持人员提供服务，威斯康星大学综合癌症中心将提供。总而言之，该应用程序将满足威斯康星大学校园内广泛的NIH资助科学界的需求，并将为当前可用于荧光分析的技术提供了重大进步。