LASER SCANNING CYTOMETER

激光扫描细胞仪

• 批准号: 6878180
• 负责人: Anna Huttenlocher
• 金额: $ 38.12万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2006-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6878180
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): This application requests funding for a CompuCyte laser scanning cytometer optimized for quantitative analysis of fluorescence in fixed and living cells and tissues. The laser scanning cytometer will become part of the University of Wisconsin Comprehensive Cancer Center's flow cytometry facility, and will serve the entire University of Wisconsin research community. The facility will serve investigators from multiple departments throughout the University of Wisconsin campus who need the instrument to quantify fluorescence in homogenous and heterogeneous cell populations with both live and fixed samples. The major users will require the CompuCyte laser scanning cytometer to (1) provide quantification of fluorescence in adherent cells and (2) analyze heterogeneous populations of cells within mixed populations or tissues. All major users have experience in fluorescence microscopy and flow cytometry analysis and have determined that the equipment currently available at the University of Wisconsin cannot meet their needs. The laser scanning cytometer will be installed in a flow cytometry facility laboratory located near the laboratory of the PI at a location central to the other investigators. Personnel experienced in flow cytometry will staff the facility and financial support for the facility will be provided by the University of Wisconsin Comprehensive Cancer Center. In summary, the application will serve the needs of a broad NIH-funded scientific community at the University of Wisconsin campus and will provide a significant advance over the current technology available for fluorescence analysis.

描述（由申请人提供）：本申请要求为优化的CompuCyte激光扫描细胞仪提供资金，用于定量分析固定和活细胞和组织中的荧光。激光扫描细胞仪将成为威斯康星州综合癌症中心的流式细胞仪设施的一部分，并将服务于整个威斯康星州研究社区的大学。该设施将为来自威斯康星州校园多个部门的研究人员提供服务，他们需要该仪器来定量活样本和固定样本的同质和异质细胞群中的荧光。主要用户将需要CompuCyte激光扫描细胞仪（1）提供贴壁细胞中荧光的定量和（2）分析混合细胞群或组织中的异质细胞群。所有主要用户都有荧光显微镜和流式细胞仪分析的经验，并已确定威斯康星州大学目前可用的设备不能满足他们的需求。激光扫描细胞仪将安装在流式细胞仪设施实验室中，该实验室位于PI实验室附近，位于其他研究者的中心位置。在流式细胞术方面有经验的人员将为该设施工作，该设施的财政支持将由威斯康星州大学综合癌症中心提供。总之，该应用程序将满足威斯康星州校园内NIH资助的广泛科学界的需求，并将提供比当前荧光分析技术的重大进步。