Triple Quadrupole Mass Spectrometer for Metabolomics Analysis

用于代谢组学分析的三重四极杆质谱仪

• 批准号: 8051470
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 44.6万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051470
• 关键词: Adult; Aging; Amyotrophic Lateral Sclerosis; Area; Biochemical; Biology; Biomedical Research; Cardiovascular Diseases; Citric Acid Cycle; Complement; Data Quality; Defect; Diabetes Mellitus; Discipline; Funding; Funding Applicant; Health; Health Sciences; High Pressure Liquid Chromatography; Housing; Institution; Investigation; Laboratories; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Metabolic; Neurosciences; Nitric Oxide Synthase; Obesity; Proteins; Regenerative Medicine; Research; Research Activity; Research Personnel; Services; South Texas; Standardization; System; Texas; United States National Institutes of Health; Universities; Work; base; design; experience; healthy aging; instrumentation; mass spectrometer; metabolomics; mother nutrition; offspring; programs; research facility

DESCRIPTION (provided by applicant): Funds are requested to purchase a triple quadrupole mass spectrometer and HPLC system for targeted quantitative metabolomics analysis at the University of Texas Health Science Center at San Antonio (UTHSCSA), under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. The requested instrumentation will provide essential analytical capabilities for a new UTHSCSA metabolomics laboratory that is being established in the South Texas Research Facility (STRF) that is currently under construction and expected to be completed in May 2011. The STRF has been designed to maximize thematically-based collaborative efforts and will house the research activities of approximately 50 investigators working in the following general areas: adult cancer, diabetes and metabolic biology, healthy aging, neuroscience, and regenerative medicine. The core laboratories that will be housed in the STRF will be available for collaborative and support services to all UTHSCSA investigators and to researchers at other institutions. Acquisition of the requested mass spectrometer and HPLC system affords a tremendous opportunity to advance the research efforts of a large number of investigators studying a variety of important NIH-funded, health-related topics, including aging, amyotrophic lateral sclerosis (ALS), cancer, cardiovascular disease, diabetes, obesity, placental function, and the consequences of maternal nutrition on short-term and long-term offspring health. In addition, a variety of key biochemical systems will be investigated, such as metabolic defects in the tricarboxylic acid cycle and the nitric oxide synthase system. Addition of metabolomics analyses to these established research programs is essential to fully answer many of the hypotheses posed and to provide guidance for new directions to be explored. Setting up a laboratory that focuses on quantitative measurements will facilitate maintenance of the necessary standardization practices that are essential for assuring high-quality data. The activities of the new metabolomics laboratory will complement the efforts of the main campus mass spectrometry facility that concentrate on protein analyses. The acquisition of triple quadrupole mass spectrometer and HPLC system for targeted quantitative analysis has the potential to positively impact a large number of NIH-funded investigations covering the full array of disciplines in biomedical research.

描述（由申请人提供）： 申请资金在圣安东尼奥德克萨斯大学健康科学中心 (UTHSCSA) 购买三重四极杆质谱仪和 HPLC 系统，用于目标定量代谢组学分析，由 Susan Weintraub 博士指导，她在生物医学质谱领域拥有 30 多年的经验。所要求的仪器将为新的 UTHSCSA 代谢组学实验室提供必要的分析能力，该实验室正在南德克萨斯研究设施 (STRF) 中建立，该实验室目前正在建设中，预计将于 2011 年 5 月竣工。STRF 旨在最大限度地发挥基于主题的合作努力，并将容纳大约 50 名研究人员在以下一般领域开展研究活动：成人癌症、糖尿病和代谢生物学、健康老龄化、 神经科学和再生医学。位于 STRF 的核心实验室将为所有 UTHSCSA 研究人员和其他机构的研究人员提供协作和支持服务。获得所需的质谱仪和 HPLC 系统为推进大量研究人员的研究工作提供了巨大的机会，这些研究人员研究由 NIH 资助的各种重要的健康相关主题，包括衰老、肌萎缩侧索硬化症 (ALS)、癌症、心血管疾病、糖尿病、肥胖、胎盘功能以及母体营养对短期和长期后代的影响 健康。此外，还将研究各种关键的生化系统，例如三羧酸循环和一氧化氮合酶系统中的代谢缺陷。在这些已建立的研究项目中添加代谢组学分析对于充分回答所提出的许多假设并为探索新方向提供指导至关重要。建立一个专注于定量测量的实验室将有助于维护必要的标准化实践，这对于确保高质量数据至关重要。新代谢组学实验室的活动将补充主校区质谱设施专注于蛋白质分析的工作。收购用于目标定量分析的三重四极杆质谱仪和 HPLC 系统有可能对 NIH 资助的涵盖生物医学研究各个学科的大量研究产生积极影响。