Triple Quadrupole Mass Spectrometer for Metabolomics Analysis

用于代谢组学分析的三重四极杆质谱仪

• 批准号: 8051470
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 44.6万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051470
• 关键词: Adult; Aging; Amyotrophic Lateral Sclerosis; Area; Biochemical; Biology; Biomedical Research; Cardiovascular Diseases; Citric Acid Cycle; Complement; Data Quality; Defect; Diabetes Mellitus; Discipline; Funding; Funding Applicant; Health; Health Sciences; High Pressure Liquid Chromatography; Housing; Institution; Investigation; Laboratories; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Metabolic; Neurosciences; Nitric Oxide Synthase; Obesity; Proteins; Regenerative Medicine; Research; Research Activity; Research Personnel; Services; South Texas; Standardization; System; Texas; United States National Institutes of Health; Universities; Work; base; design; experience; healthy aging; instrumentation; mass spectrometer; metabolomics; mother nutrition; offspring; programs; research facility

DESCRIPTION (provided by applicant): Funds are requested to purchase a triple quadrupole mass spectrometer and HPLC system for targeted quantitative metabolomics analysis at the University of Texas Health Science Center at San Antonio (UTHSCSA), under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. The requested instrumentation will provide essential analytical capabilities for a new UTHSCSA metabolomics laboratory that is being established in the South Texas Research Facility (STRF) that is currently under construction and expected to be completed in May 2011. The STRF has been designed to maximize thematically-based collaborative efforts and will house the research activities of approximately 50 investigators working in the following general areas: adult cancer, diabetes and metabolic biology, healthy aging, neuroscience, and regenerative medicine. The core laboratories that will be housed in the STRF will be available for collaborative and support services to all UTHSCSA investigators and to researchers at other institutions. Acquisition of the requested mass spectrometer and HPLC system affords a tremendous opportunity to advance the research efforts of a large number of investigators studying a variety of important NIH-funded, health-related topics, including aging, amyotrophic lateral sclerosis (ALS), cancer, cardiovascular disease, diabetes, obesity, placental function, and the consequences of maternal nutrition on short-term and long-term offspring health. In addition, a variety of key biochemical systems will be investigated, such as metabolic defects in the tricarboxylic acid cycle and the nitric oxide synthase system. Addition of metabolomics analyses to these established research programs is essential to fully answer many of the hypotheses posed and to provide guidance for new directions to be explored. Setting up a laboratory that focuses on quantitative measurements will facilitate maintenance of the necessary standardization practices that are essential for assuring high-quality data. The activities of the new metabolomics laboratory will complement the efforts of the main campus mass spectrometry facility that concentrate on protein analyses. The acquisition of triple quadrupole mass spectrometer and HPLC system for targeted quantitative analysis has the potential to positively impact a large number of NIH-funded investigations covering the full array of disciplines in biomedical research.

描述（由申请人提供）：要求资金购买三重定量代谢组学分析的三倍四极杆质谱仪和HPLC系统，用于在Susan Weintraub博士的指导下，在得克萨斯大学圣安东尼奥大学（UTHSCSA）的德克萨斯大学健康科学中心（UTHSCSA），他们在生物典型质谱质量方面有30多年的经验。所请求的仪器将为新的UTHSCSA代谢组学实验室提供必不可少的分析能力，该实验室正在南德克萨斯州研究机构（STRF）建立，该实验室目前正在建设中，预计将于2011年5月完成。STRF旨在最大程度地提高主题性的努力，并将在下面的研究活动中最大程度地提高癌症，并将在健康的研究活动中受到研究：成人癌症的研究，并在健康的研究活动中，并伴随着成人的癌症，并伴随着癌症，并将其纳入癌症。神经科学和再生医学。将在STRF中安置的核心实验室将用于所有UTHSCSA调查人员以及其他机构的研究人员的协作和支持服务。获得所需的质谱仪和HPLC系统的获取为促进许多研究人员的研究工作提供了巨大的机会，这些研究人员研究了各种重要的NIH资助，与健康相关的主题，包括衰老，肌萎缩性侧索硬化症（ALS），癌症，癌症，心血管疾病，癌症，妇产科，持续性，阶层功能，孕产，以及途中的临时功能，跨性别，以及后果，以及后果，以及后果，以及后果，以及后果，以及后果，妇女及其后果，以及途中的影响力，及时的影响力， 健康。此外，还将研究各种关键的生化系统，例如三羧酸周期中的代谢缺陷和一氧化氮合酶系统。在这些既定研究计划中添加代谢组学分析对于充分回答许多假设并为探索新方向提供指导至关重要。建立一个专注于定量测量的实验室将有助于维持必要的标准化实践，这对于确保高质量数据至关重要。新代谢组学实验室的活动将补充主要校园质谱设施的工作，该设施集中在蛋白质分析上。对靶向定量分析的三倍四极杆质谱仪和HPLC系统的采集具有积极影响的大量NIH资助的研究，这些研究涵盖了生物医学研究中的全部学科。