MASS SPECTROMETRY SHARED RESOURCE

质谱学(质谱学)共享资源提供了广泛的分析 支持UTHSCSA和In癌症中心研究人员和其他研究人员的需求的能力 附近的社区。质谱仪在质谱学共享中的应用 来源如下：Thermo Fisher LTQ离子陷阱串联质谱仪与 一种具有代表性的纳米LC微型高效液相系统，用于进行纳米喷雾高效液相色谱/质谱分析. 分析.与密克罗姆一起使用的Thermo Fisher Quantum-AM三重四极质谱计 生物资源模式质谱仪SPECTR0SC0PY4微反相高效液相系统 与Michrom BioResources范例质谱仪结合使用的质谱计 SPECTR0SC0PY4微液相色谱系统；应用生物合成质谱学Voyager DE-STR基质辅助 激光解吸电离飞行时间质谱仪(MALDI-TOF/质谱仪 光谱学)；能够执行的Thermo Fisher DSQ四极质谱计 电子轰击和化学电离的GC/MS和直接探针分析 正、负离子检测。除了质谱计之外，这些设备还可以帮助 在该设施中可以进行蛋白质表征，包括成像仪、密度计、凝胶斑点切割器和 PDQuest凝胶分析软件(全部来自Bio-Rad)和SameSpots凝胶分析软件 动力学。提供的主要蛋白质分析服务包括凝胶电泳、分子质量分析。 测定，从溶液或聚丙烯酰胺凝胶中鉴定蛋白质，序列特征，以及 阐明跨膜后修饰的部位。亚皮摩尔范围内的检测限值为 粗略地获得，使从生物中分离的蛋白质和肽的特征成为可能 样本。用气相色谱/质谱法对脂类和中间代谢物进行了定量分析 也定期演出。

The Mass Spectrometry (MASS SPECTROSCOPY) Shared Resource offers a wide range of analytical capabilities to support the needs of Cancer Center investigators and other researchers at UTHSCSA and in the nearby community. Mass spectrometry instrumentafion in the MASS SPECTROSCOPY Shared Resource is as follows: Thermo Fisher LTQ ion trap tandem mass spectrometer used in conjunction with an Eksigent NanoLC micro HPLC system to perform nanospray HPLC/MASS SPECTROSCOPY" analyses; Thermo Fisher Quantum-AM triple quadrupole mass spectrometer used with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Thermo Fisher LCQ ion trap mass spectrometer used in conjunction with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Applied BiosysteMass Spectroscopy Voyager DE-STR matrixassisted laser desorpfion ionization fime-of-flight mass spectrometer (MALDI-TOF/MASS SPECTROSCOPY); Thermo Fisher DSQ quadrupole mass spectrometer which is able to perform GC/MASS SPECTROSCOPY and direct probe analyses using electron impact and chemical ionizafion with positive and negafive ion detecfion. In addition to the mass spectrometers, devices that greafiy facilitate protein characterization are available in the facility, including an imager, densitometer, gel spotcutter and PDQuest gel analysis software (all from Bio-Rad) and SameSpots gel analysis software from Nonlinear Dynamics. The major protein analysis services provided include gel electrophoresis, molecular mass determinafion, protein idenfificafion from solufion or polyacrylamide gel, sequence characterizafion, and elucidation of sites of post-translafional modificafion. Detecfion limits in the sub-picomole range are roufinely obtained, making it possible to characterize proteins and pepfides isolated from biological samples. Quantitative analysis of lipids and intermediary metabolites by GC/MASS SPECTROSCOPY is also performed on a regular basis.