MASS SPECTROMETRY SHARED RESOURCE

质谱共享资源

• 批准号: 7944762
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 2.47万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-03 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944762
• 关键词: Amino Acids; Anus; Biological; Cancer Center; Categories; Cells; Chemicals; Chromatography; Citric Acid Cycle; Communities; Complex Mixtures; Computer software; Devices; Diamond; Digestion; Electrons; Excision; Exhibits; Fluorescent Dyes; Gases; Gel; High Pressure Liquid Chromatography; Housing; Image Analysis; Individual; Ions; Label; Lasers; Length; Licensing; Lipids; Mass Spectrum Analysis; Measures; Methods; Molecular Weight; Nonlinear Dynamics; Nucleic Acid Databases; Peptides; Phase; Phosphoproteins; Phosphorylation; Pro-Q aerosol foam; Protein Analysis; Proteins; Proteome; Proteomics; Relative (related person); Research; Research Personnel; Resource Sharing; Running; Sampling; Science; Sequence Analysis; Services; Site; Slice; Solid; Solutions; Source; Spectrum Analysis; Spottings; Stable Isotope Labeling; Staging; Staining method; Stains; Subcellular Fractions; System; Therapeutic Studies; Time; Tissues; Two-Dimensional Gel Electrophoresis; base; cancer therapy; comparison group; cyanine dye 5; gel electrophoresis; instrument; ionization; mass spectrometer; mass spectrometry shared resource; member; molecular mass; polyacrylamide gels; programs; protein expression; quantum; research study; scaffold; selected ion monitoring; small molecule; stable isotope

The Mass Spectrometry (MASS SPECTROSCOPY) Shared Resource offers a wide range of analytical capabilities to support the needs of Cancer Center investigators and other researchers at UTHSCSA and in the nearby community. Mass spectrometry instrumentafion in the MASS SPECTROSCOPY Shared Resource is as follows: Thermo Fisher LTQ ion trap tandem mass spectrometer used in conjunction with an Eksigent NanoLC micro HPLC system to perform nanospray HPLC/MASS SPECTROSCOPY" analyses; Thermo Fisher Quantum-AM triple quadrupole mass spectrometer used with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Thermo Fisher LCQ ion trap mass spectrometer used in conjunction with a Michrom BioResources Paradigm MASS SPECTR0SC0PY4 micro HPLC system; Applied BiosysteMass Spectroscopy Voyager DE-STR matrixassisted laser desorpfion ionization fime-of-flight mass spectrometer (MALDI-TOF/MASS SPECTROSCOPY); Thermo Fisher DSQ quadrupole mass spectrometer which is able to perform GC/MASS SPECTROSCOPY and direct probe analyses using electron impact and chemical ionizafion with positive and negafive ion detecfion. In addition to the mass spectrometers, devices that greafiy facilitate protein characterization are available in the facility, including an imager, densitometer, gel spotcutter and PDQuest gel analysis software (all from Bio-Rad) and SameSpots gel analysis software from Nonlinear Dynamics. The major protein analysis services provided include gel electrophoresis, molecular mass determinafion, protein idenfificafion from solufion or polyacrylamide gel, sequence characterizafion, and elucidation of sites of post-translafional modificafion. Detecfion limits in the sub-picomole range are roufinely obtained, making it possible to characterize proteins and pepfides isolated from biological samples. Quantitative analysis of lipids and intermediary metabolites by GC/MASS SPECTROSCOPY is also performed on a regular basis.

质谱（MASS SPECTROSCOPY）共享资源提供广泛的分析 能力，以支持癌症中心的研究人员和其他研究人员在UTHSCSA和在 附近的社区。质谱仪器在质谱学中的应用 资源如下：Thermo Fisher LTQ离子阱串联质谱仪与 Eksigent NanoLC微型HPLC系统，用于执行纳米喷雾HPLC/质谱” 分析; Thermo Fisher Quantum-AM三重四极杆质谱仪与Michrom BioResources Paradigm MASS SPECTR 0 SC 0 PY 4微型HPLC系统; Thermo Fisher LCQ离子阱 与Michrom BioResources Paradigm MASS结合使用的质谱仪 SPECTR 0 SC 0 PY 4微型HPLC系统; Applied Biosystem质谱Voyager DE-STR基质辅助 激光解吸电离飞行时间质谱仪 SPECTROSCOPY）; Thermo Fisher DSQ四极杆质谱仪，能够执行 GC/MS光谱和直接探针分析，使用电子碰撞和化学电离， 正负离子检测。除了质谱仪之外，还可以使用一些设备， 蛋白质表征可在该设施，包括成像仪，密度计，凝胶点切割器， PDQuest凝胶分析软件（均来自Bio-Rad）和SameSpots凝胶分析软件（来自Nonlinear） 动力学主要提供的蛋白质分析服务包括凝胶电泳、分子量分析、 测定，从溶液或聚丙烯酰胺凝胶中进行蛋白质电泳，序列表征，以及 阐明后修饰位点。亚皮摩尔范围内的检测极限为 获得的蛋白质和肽，使得可以表征从生物样品中分离的蛋白质和肽。 样品通过GC/MS光谱法对脂质和中间代谢物进行定量分析。 也定期进行。