Orbitrap XL/ETD Mass Spectrometer

Orbitrap XL/ETD 质谱仪

• 批准号: 7498740
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 77.61万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-06 至 2010-04-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7498740
• 关键词: Area; Bacteriophages; Biochemical; Biomedical Research; Blood capillaries; Cardiovascular Diseases; Charge; Citric Acid Cycle; Complex; Computer Systems; Defect; Diabetes Mellitus; Discipline; Electron Transport; Enzymes; Funding; Funding Applicant; Future; Health Sciences; High Pressure Liquid Chromatography; Individual; Investigation; Ions; Laboratories; Mass Spectrum Analysis; Mediating; Metabolism; Mitochondria; Modification; NADPH-Ferrihemoprotein Reductase; Nitric Oxide Synthase; Peptides; Post-Translational Protein Processing; Proteins; Relative (related person); Research; Research Personnel; Resolution; Site; System; Texas; Time; Universities; alcohol exposure; capillary; cell assembly; experience; fetus nutrition; instrument; mass spectrometer; public health relevance; research study

DESCRIPTION (provided by applicant): Funds are requested to purchase a Thermo Fisher LTQ Orbitrap XL mass spectrometer, a micro HPLC system and computer cluster hardware for the Institutional Mass Spectrometry Laboratory of the University of Texas Health Science Center at San Antonio, under the direction of Dr. Susan Weintraub, who has over 30 years experience in biomedical mass spectrometry. At the present time, capillary HPLC-ESIMS/ MS analyses are conducted on a heavily-utilized Thermo Fisher LTQ that was installed in October 2005. There are no high resolution/high mass accuracy mass spectrometers in San Antonio. The major uses for the requested instrument will be for assessing relative differences in protein quantities and for identifying sites of protein modification. The projects of nine major users and two future users are presented. Medically-relevant areas that will be studied by the major users include cardiovascular disease, diabetes, ethanol exposure and maternal/fetal nutrition; in addition, a wide variety of key biochemical systems will be investigated, such as bacteriophage assembly, cell contact-mediated processes, metabolic defects due to loss of individual TCA cycle enzymes, NADPH-cytochrome P450 oxidoreductase, nitric oxide synthase and modification of mitochondrial electron transport complexes. The requested Orbitrap XL will enhance the projects of the user group in many ways. The acquisition of high mass accuracy precursor ion spectra will substantially lower false discovery rates, leading to higher confidence for protein identifications. High mass resolution will be essential for charge state determinations and for differentiating between nearly isobaric species. The availability of multiple fragmentation strategies (CAD, HCD and ETD) will result in more successful localization of sites of modification and greater sequence coverage for protein identification/characterization. Finally, the capability of top-down analysis in conjunction with ETD will facilitate comprehensive characterization of large peptides and small proteins. In addition to the proposed user group, availability of the Orbitrap will stimulate other investigators to plan new experiments in the future that may take their research endeavors into exciting new directions. Thus, acquisition of the Orbitrap has the potential to positively impact a large number of NIH-funded investigations covering the full array of disciplines in biomedical research. PUBLIC HEALTH RELEVANCE Acquisition of the requested Orbitrap XL mass spectrometer will advance the research efforts of nine major users in six different departments at the University of Texas Health Science Center at San Antonio. Medically-relevant areas that will be studied by the major users include cardiovascular disease, diabetes, ethanol exposure and maternal/fetal nutrition; in addition, a wide variety of key biochemical systems will be investigated. Beyond the proposed user group, availability of the Orbitrap will stimulate other investigators to plan new experiments in the future that may take their biomedical research endeavors into exciting new directions.

描述（由申请人提供）：申请资金用于为圣安东尼奥德克萨斯大学健康科学中心的机构质谱实验室购买Thermo Fisher LTQ Orbitrap XL质谱仪、微型HPLC系统和计算机集群硬件，该实验室由Susan Weintraub博士指导，Susan Weintraub博士在生物医学质谱方面拥有30多年的经验。目前，毛细管HPLC-ESIMS/ MS分析是在2005年10月安装的大量使用的Thermo Fisher LTQ上进行的。圣安东尼奥没有高分辨率/高质量准确度质谱仪。所要求的仪器的主要用途是评估蛋白质数量的相对差异和鉴定蛋白质修饰的位点。介绍了九个主要用户和两个未来用户的项目。主要用户将研究的与医学有关的领域包括心血管疾病、糖尿病、酒精接触和母亲/胎儿营养;此外，还将研究各种关键的生化系统，如噬菌体组装、细胞接触介导的过程、由于个别TCA循环酶、NADPH-细胞色素P450氧化还原酶一氧化氮合酶和线粒体电子传递复合物的修饰。所要求的Orbitrap XL将在许多方面加强用户组的项目。高质量准确度前体离子光谱的获取将大大降低错误发现率，从而提高蛋白质鉴定的置信度。高质量分辨率将是必不可少的电荷状态的测定和区分近同量异位素的物种。多种片段化策略（CAD、HCD和ETD）的可用性将导致修饰位点的更成功的定位和用于蛋白质鉴定/表征的更大的序列覆盖。最后，自上而下的分析能力与ETD相结合，将促进大肽和小蛋白质的全面表征。除了拟议的用户群外，轨道阱的可用性将刺激其他研究人员计划未来的新实验，这些实验可能会将他们的研究努力带入令人兴奋的新方向。因此，收购Orbitrap有可能对大量NIH资助的研究产生积极影响，这些研究涵盖了生物医学研究的全部学科。公共卫生相关性收购所要求的Orbitrap XL质谱仪将推进圣安东尼奥得克萨斯大学健康科学中心六个不同部门的九个主要用户的研究工作。主要用户将研究的医学相关领域包括心血管疾病、糖尿病、乙醇暴露和母亲/胎儿营养;此外，还将研究各种关键的生化系统。除了拟议的用户群之外，Orbitrap的可用性将刺激其他研究人员计划未来的新实验，这些实验可能会将他们的生物医学研究努力带入令人兴奋的新方向。

项目成果

Identification of Essential Genes in the Salmonella Phage SPN3US Reveals Novel Insights into Giant Phage Head Structure and Assembly.

• DOI: 10.1128/jvi.01492-16
• 发表时间: 2016-11-15
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Thomas JA;Benítez Quintana AD;Bosch MA;Coll De Peña A;Aguilera E;Coulibaly A;Wu W;Osier MV;Hudson AO;Weintraub ST;Black LW
• 通讯作者: Black LW

Characterization of the Primary Human Trophoblast Cell Secretome Using Stable Isotope Labeling With Amino Acids in Cell Culture.

• DOI: 10.3389/fcell.2021.704781
• 发表时间: 2021
• 期刊: Frontiers in cell and developmental biology
• 影响因子: 5.5
• 作者: Rosario FJ;Pardo S;Michelsen TM;Erickson K;Moore L;Powell TL;Weintraub ST;Jansson T
• 通讯作者: Jansson T

Major venom proteins of the fire ant Solenopsis invicta: insights into possible pheromone-binding function from mass spectrometric analysis.

• DOI: 10.1111/imb.12388
• 发表时间: 2018-08
• 期刊: Insect molecular biology
• 影响因子: 2.6
• 作者: Das T;Alabi I;Colley M;Yan F;Griffith W;Bach S;Weintraub ST;Renthal R
• 通讯作者: Renthal R

Mass Spectral Analyses of Salmonella Myovirus SPN3US Reveal Conserved and Divergent Themes in Proteolytic Maturation of Large Icosahedral Capsids.

• DOI: 10.3390/v15030723
• 发表时间: 2023-03-10
• 期刊: Viruses
• 作者: Scheuch A;Moran SAM;Faraone JN;Unwin SR;Vu G;Benítez AD;Mohd Redzuan NH;Molleur D;Pardo S;Weintraub ST;Thomas JA
• 通讯作者: Thomas JA

To Be or Not To Be T4: Evidence of a Complex Evolutionary Pathway of Head Structure and Assembly in Giant Salmonella Virus SPN3US.

• DOI: 10.3389/fmicb.2017.02251
• 发表时间: 2017
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Ali B;Desmond MI;Mallory SA;Benítez AD;Buckley LJ;Weintraub ST;Osier MV;Black LW;Thomas JA
• 通讯作者: Thomas JA