ESI-TOF Mass Spectrometer for Molecular Mass Analysis: Proteins/Small Molecules

用于分子质量分析的 ESI-TOF 质谱仪：蛋白质/小分子

• 批准号: 8447919
• 负责人: SUSAN T WEINTRAUB
• 金额: $ 16.53万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-19 至 2014-07-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447919
• 关键词: Agreement; Biochemistry; Biological Factors; Biomedical Research; Complement; Discipline; Environment; Evaluation; Funding; Funding Applicant; Health Sciences; Investigation; Laboratories; Laboratory Personnel; Marketing; Mass Spectrum Analysis; Minor; Peptides; Production; Protein Analysis; Proteins; Proteomics; Research Personnel; Research Project Grants; Sampling; Services; Site; Sodium Dodecyl Sulfate-PAGE; Solutions; Structural Protein; Students; Supervision; Texas; Time; Training; United States National Institutes of Health; Universities; Western Blotting; cancer therapy; cost effective; effective therapy; instrument; mass spectrometer; molecular mass; small molecule

DESCRIPTION (provided by applicant): Funds are requested to purchase an Agilent 6224 ESI-TOF mass spectrometer for molecular mass determination of proteins, peptides and "small" molecules for investigators at the University of Texas Health Science Center at San Antonio (UTHSCSA). The requested instrument will add valuable new capabilities to complement the existing Mass Spectrometry Laboratory (MSL) that has offered a wide range of proteomics services for many years. The impetus for initiating the effort to obtain an ESI-TOF mass spectrometer came from the establishment of a protein production facility in the UTHSCSA Department of Biochemistry that was set up to help investigators who require relatively large quantities of purified proteins for structural/biophysical studies but do not have the capacity in their own laboratory to generate the requisite protein. Initial exploration of ESI-TOF/MS instruments on the market took place in mid 2010. Because sufficient funds were not available at that time to purchase an instrument, representatives from Agilent approached the PI about the possibility of an on-site evaluation of an ESI-TOF/MS. An Agilent 6224 was installed in the MSL in summer 2011 and was integrated into the laboratory workflow several months later. The instrument has quickly become essential for support of many research projects, in particular, an NIH-funded investigation focusing on discovery of new natural products and synthetic compounds to provide more effective therapies for the treatment of cancer. Investigators who have had protein samples analyzed on the Agilent 6224 now recognize the substantial benefit of obtaining accurate and more comprehensive molecular mass information about their proteins as compared to the far less precise assessment provided by 1-D SDS PAGE and Western blotting. If this proposal is funded, the instrument will be made available in an open-access environment such that after proper training, staff/students from the users' laboratories will be able to conduc their own analyses with only minor supervision from MSL personnel. Analyses on the requested ESI-TOF mass spectrometer will be much more accurate, more cost-effective and with a faster turn-around time than is currently available through the MSL, and will be amenable to samples in a wider range of solution compositions. The agreement with Agilent is for a one-year evaluation, although it is hoped that the time can be extended until the review of this proposal is completed. The acquisition of an ESI-TOF mass spectrometer for molecular mass determination has the potential to positively impact a large number of NIH-funded investigations covering the full array of disciplines in biomedical research.

描述（由申请人提供）：需要资金购买 Agilent 6224 ESI-TOF 质谱仪，用于为圣安东尼奥德克萨斯大学健康科学中心 (UTHSCSA) 的研究人员测定蛋白质、肽和“小”分子的分子质量。所需的仪器将增加有价值的新功能，以补充现有的质谱实验室 (MSL)，该实验室多年来一直提供广泛的蛋白质组学服务。开始努力获得 ESI-TOF 质谱仪的动力来自于 UTHSCSA 生物化学系建立的蛋白质生产设施，该设施的设立是为了帮助需要相对大量纯化蛋白质进行结构/生物物理研究但在自己的实验室中没有能力生成所需蛋白质的研究人员。市场上对 ESI-TOF/MS 仪器的初步探索发生在 2010 年中期。由于当时没有足够的资金来购买仪器，安捷伦的代表向 PI 询问了对 ESI-TOF/MS 进行现场评估的可能性。 Agilent 6224 于 2011 年夏季安装在 MSL 中，并在几个月后集成到实验室工作流程中。该仪器很快就成为支持许多研究项目的必需品，特别是 NIH 资助的一项研究，重点是发现新的天然产物和合成化合物，以提供更有效的癌症治疗方法。在 Agilent 6224 上分析蛋白质样品的研究人员现在认识到，与 1-D SDS PAGE 和蛋白质印迹提供的不太精确的评估相比，获得有关蛋白质的准确且更全面的分子量信息具有巨大的好处。如果该提案获得资助，该仪器将在开放式环境中提供，这样经过适当的培训，用户实验室的工作人员/学生将能够在 MSL 人员的少量监督下进行自己的分析。与目前通过 MSL 进行的分析相比，所要求的 ESI-TOF 质谱仪的分析将更加准确、更具成本效益且周转时间更快，并且适用于更广泛的溶液成分中的样品。与安捷伦达成的协议是为期一年的评估，不过希望可以延长时间，直到对该提案的审查通过。 完全的。购买用于分子质量测定的 ESI-TOF 质谱仪有可能对 NIH 资助的涵盖生物医学研究各个学科的大量研究产生积极影响。