Amersham Typhoon 9410 Variable Model Imager

Amersham Typhoon 9410 变量模型成像仪

• 批准号: 6877605
• 负责人: J. Michael MCCAFFERY
• 金额: $ 13.74万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877605
• 关键词: autoradiography; bioimaging /biomedical imaging; biomedical equipment purchase; biomedical equipment resource; fluorescence; imaging /visualization /scanning; luminescence; phosphorescence

DESCRIPTION (provided by applicant): A diverse user-group of 10 NIH funded investigators, within the Department of Biology at The Johns Hopkins University, request funds to purchase an Amersham 9410 Typhoon Variable Mode Imager to replace an outmoded 1993 Molecular Dynamics SF Model 455, which has been the primary workhorse for the department since 1993. This state of- the-art Typhoon phosophorimager will replace and constitute a significant upgrade from our older SF 455 system in terms of capabilities and, most importantly, reliability. Department investigators, all well funded through the NIH, work on a host of basic cell biological/biochemical related questions including, though not limited to, calcium signaling in yeast; helicases and bacterial conjugation, regulation of transcription and DNA binding by proteins, glycose phosphotransferase system, membrane organization and dynamics; microtubule-based motors; membrane trafficking; germ cell development and migration; sensory neuron development; and mechanisms of endocytosis. In the requested configuration, the 9410 phosophorimager will afford our disparate group of investigators a broad range of basic/advance capabilities for imaging/quantifying gel sandwiches, full-sized agarose and polyacrylamide gels, membranes, microplates, and microarrays. These capabilities required by our investigators are absent or deficient on our current SF 455 system, a limited format system. An even more critical concern in this submission pertains to the age-related unreliability of our older SF 455 system; and that Amersham has discontinued supplying service/parts to maintain the system in competent working condition. Particular worrisome is that the MD 455 design incorporates a unique, un-replacable integral computer design for which parts have been progressively more difficult to acquire, reinforcing our concern that in the near future the system will become un-repairable leaving us with no system. An additional major consideration relates to the rather costly/messy maintenance of the film processor and safe handling/disposal of photographic chemicals. Consequently, given the essential nature of gels/blots to our basic biological/biochemical research and the evolving use of microarray analysis in genomics/proteomics by department investigators; coupled with escalating concerns pertaining to reliability and performance limitations of our current system; we are seeking to upgrade our common-use facilities with the addition of the 9410 variable mode imager. The Typhoon 9410 will be conveniently located, administered, and maintained, in the Department's Integrated Imaging Center (IIC); and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

描述（由申请人提供）：一个由10名NIH资助的研究人员组成的多元化用户组，在约翰霍普金斯大学生物系内，申请资金购买阿默舍姆9410台风可变模式成像仪，以取代过时的1993年分子动力学SF模型455，该模型自1993年以来一直是该部门的主要主力。这台最先进的Typhoon Phosophorimager将取代我们的旧SF 455系统，并在功能和最重要的可靠性方面进行重大升级。部门的研究人员，所有通过美国国立卫生研究院的资金，工作在一个基本的细胞生物学/生物化学相关的问题，包括主机，但不限于，在酵母钙信号;解旋酶和细菌接合，转录和蛋白质，葡萄糖磷酸转移酶系统，膜组织和动力学DNA结合的调节;微管为基础的马达;膜运输;生殖细胞的发育和迁移;感觉神经元的发育和内吞作用的机制。在所要求的配置中，9410感光成像仪将为我们不同的研究人员团队提供广泛的基本/高级功能，用于成像/量化凝胶三明治、全尺寸琼脂糖和聚丙烯酰胺凝胶、膜、微孔板和微阵列。我们目前的SF 455系统（一个有限格式的系统）缺乏或缺乏研究者所需的这些功能。 本提交资料中更重要的问题是，我们的旧SF 455系统存在老化相关的不可靠性;阿默舍姆已停止提供服务/部件，以维持系统处于合格的工作状态。特别令人担忧的是，MD 455的设计采用了一种独特的、不可拆卸的整体计算机设计，其部件越来越难以获得，这加强了我们的担忧，即在不久的将来，该系统将变得不可修理，使我们没有系统。另一个主要的考虑因素涉及到胶片处理机的相当昂贵/混乱的维护和摄影化学品的安全处理/处置。因此，鉴于凝胶/印迹的基本性质，我们的基础生物/生化研究和不断发展的使用微阵列分析的基因组学/蛋白质组学部门的研究人员，再加上不断升级的关注有关的可靠性和性能限制，我们目前的系统，我们正在寻求升级我们的共同使用的设施，除了9410可变模式成像仪。 台风9410号将在该部门的综合成像中心（IIC）进行方便的定位，管理和维护;它将被纳入IIC现有的完善的补给系统，以确保收回供应和维护资金。