ZEISS/31 STALLION DDI MULTI-DIMENSIONAL LIVE-CELL IMAGING WORKSTATION

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：由11名NIH资助的研究人员组成的多元化小组，来自约翰霍普金斯大学艺术与科学、工程、医学和公共卫生学院；和马里兰大学医学院的马里兰大学生物技术研究所向卡尔蔡司/智能成像创新公司申请资金购买一台种马DPI活细胞数字成像工作站。该系统集成了蔡司200M红外平台和双超高速/高灵敏度级联512B EM数码相机；405/488 FRAP/光激活激光模块；完全集成31个幻灯片多维采集/分析软件；并针对1)快速/超灵敏4D双通道采集进行了优化；2)反褶积;3) FRET和比值成像；4)粒子跟踪；5) FRAP和光活化。新的最先进的工作站将补充我们大量使用的蔡司LSM 510 METAs和老化的Deltavision系统，同时在速度和灵敏度方面进行重大升级。重要的是，Stallion DPI将为霍普金斯大学综合成像中心提供新的/独特的宽视场荧光功能，该中心是霍默伍德校园/霍普金斯大学范围内的显微镜资源，由60个实验室和200个用户组成的多个学校和部门定期使用。我们的研究人员都是由美国国立卫生研究院资助的，他们研究一系列基本的细胞生物学、生物化学和生物分子相关问题，包括但不限于：肿瘤中的端粒/着丝粒重组；HSV的组装和成熟；MHC I的运输和组装，膜组织和动力学；内体/溶酶体动力学;膜贩运;聚合基因载体的贩运；线粒体与凋亡；唾液酸显示与细胞粘附；以及内吞作用的机制。在要求的配置中，Stallion DDI工作站将为我们不同的研究小组提供广泛的基本/高级活细胞显微镜功能，这是目前霍普金斯大学霍姆伍德校区缺乏的。新系统将位于生物系综合成像中心（NO:），便于管理和维护，并将纳入IIC现有的完善的充电系统，以确保回收供应和维护的资金。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.