Ti:Sapphire Laser Upgrade to Zeiss LSM 510/Confocor 3 FCS System

Ti:Sapphire 激光器升级至 Zeiss LSM 510/Confocor 3 FCS 系统

• 批准号: 7595448
• 负责人: J. Michael MCCAFFERY
• 金额: $ 34.63万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595448
• 关键词: Arts; Biology; Biomedical Engineering; Biophysics; Carbon Dioxide; Cell Differentiation process; Cells; Cellular biology; Circadian Rhythms; Complex; Core Facility; Engineering; Ensure; Environment; Fluorescence; Funding; Gene Expression Regulation; Herpesviridae; Humidity; Image; In Vitro; Incubators; Institutes; Internet; Laboratories; Lasers; Letters; Life; Maintenance; Membrane Protein Traffic; Membrane Proteins; Microscopy; Mitochondria; Monitor; Neurosciences; Organogenesis; Psychology; Recovery; Research; Research Personnel; Resources; Sapphire; Schools; Science; Spectrum Analysis; System; Temperature; Tissues; United States National Institutes of Health; Universities; Update; Virus Assembly; Work; in vivo; instrument; interest; medical schools; nanobiotechnology; neuronal circuitry; programs; protein folding; public health relevance; research study

DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins University Krieger School of Arts and Sciences, Whiting School of Engineering, and the School of Medicine request funds to purchase a Chameleon ULTRA II Ti:Sapphire multiphoton laser; a Zeiss environmental incubator (for modulating temperature, humidity, and CO2); and NLO hardware necessary to integrate the new laser onto an existing LSM 510 VIS system equipped with a Confocor-3 Fluorescence Correlation Spectroscopy module. The resulting configuration will significantly upgrade the FCS capability of the Confocor-3; and add a new live cell/deep tissue imaging resource that is presently absent from the Homewood campus. It will be a boon to multiple ongoing research programs within biology, psychology, biophysics, engineering, and the newly expanded neuroscience presence on Homewood. The instrument is situated in the Johns Hopkins Integrated Imaging Center (IIC, http://www.jhu.edu/iic), a core facility associated with the Institute of NanoBioTechnology (INBT, http://inbt.jhu.edu/index.php). The IIC is a Homewood campus/Hopkins-wide microscopy resource, jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering (see Lattman & Jones letters, page 2 of the appendix); and utilized regularly by multiple universities, schools, and departments comprising >80 laboratories and more than 250 users. Our investigators, all well funded through the NIH, work on a host of diverse basic cell biology and biomedical engineering related questions including, though not limited to: cell differentiation and gene regulation; Herpes virus assembly and maturation, membrane trafficking, mitochondrial fission, membrane protein folding, organogenesis, and circadian rhythm neuronal circuitry. In the updated configuration, the LSM 510/Confocor 3 will afford our disparate group of investigators a broad range of basic/advanced in vivo and in vitro microscopy capabilities that are presently absent from the Hopkins Homewood campus. The new system will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance; it will be made freely available to all interested users Hopkins- wide through our convenient web-scheduler; and it will be incorporated into the IIC's annual undergraduate/graduate course offerings. PUBLIC HEALTH RELEVANCE: Ti:Sapphire Laser upgrade to LSM 510/Confocor-3 Fluorescence Correlation Spectroscopy System will be used for monitoring the interactions of fluorescent biomolecules and biomacromolecular complexes in their native sub-cellular environments; and for deep tissue and live imaging experiments.

描述（由申请人提供）：约翰·霍普金斯大学克里格艺术与科学学院、怀廷工程学院和医学院的 11 名 NIH 资助研究人员组成的多元化小组请求资金购买 Chameleon ULTRA II Ti:Sapphire 多光子激光器；蔡司环境培养箱（用于调节温度、湿度和二氧化碳）；将新激光器集成到配备 Confocor-3 荧光相关光谱模块的现有 LSM 510 VIS 系统中所需的 NLO 硬件。由此产生的配置将显着提升 Confocor-3 的 FCS 能力；并添加霍姆伍德校区目前缺乏的新活细胞/深层组织成像资源。这将有利于生物学、心理学、生物物理学、工程学以及霍姆伍德新扩展的神经科学领域的多个正在进行的研究项目。该仪器位于约翰霍普金斯大学综合成像中心（IIC，http://www.jhu.edu/iic），该中心是与纳米生物技术研究所（INBT，http://inbt.jhu.edu/index.php）相关的核心设施。 IIC 是霍姆伍德校区/霍普金斯大学范围内的显微镜资源，由克里格艺术与科学学院和怀廷工程学院共同支持（参见附录第 2 页的 Lattman 和 Jones 信件）；并被多所大学、学校和部门定期使用，其中包括超过 80 个实验室和超过 250 个用户。我们的研究人员均通过 NIH 获得充足的资助，致力于研究一系列不同的基础细胞生物学和生物医学工程相关问题，包括但不限于：细胞分化和基因调控；疱疹病毒组装和成熟、膜运输、线粒体裂变、膜蛋白折叠、器官发生和昼夜节律神经元回路。在更新的配置中，LSM 510/Confocor 3 将为我们不同的研究人员小组提供广泛的基本/高级体内和体外显微镜功能，而这些功能目前在霍普金斯大学霍姆伍德校区尚不具备。新系统将并入IIC现有的、完善的充值系统，以确保回收补给和维护资金；它将通过我们方便的网络调度程序免费提供给霍普金斯范围内所有感兴趣的用户；它将被纳入 IIC 的年度本科/研究生课程中。公共健康相关性：Ti:Sapphire 激光升级至 LSM 510/Confocor-3 荧光相关光谱系统将用于监测荧光生物分子和生物大分子复合物在其天然亚细胞环境中的相互作用；以及深层组织和实时成像实验。