FEI Tecnai F20 Transmission Electron Microscope for Core Microscopy Facility

用于岩芯显微设备的 FEI Tecnai F20 透射电子显微镜

• 批准号: 8733931
• 负责人: J. Michael MCCAFFERY
• 金额: $ 128.92万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8733931
• 关键词: Arts; Biochemical; Biological; Biology; Cell Adhesion; Cells; Chromosomes; Communities; Complement; Cryoelectron Microscopy; DNA; Detection; Dose; Electron Microscope; Endocytosis; Endosomes; Engineering; Ensure; Free Will; Funding; Future; Genes; Growth; HIV; Housing; Image; Institutes; Internet; Laboratories; Letters; Lysosomes; Maintenance; Membrane; Membrane Protein Traffic; Microscopy; Minor; Molecular; Recovery; Regulation; Research; Research Personnel; Resources; Schools; Science; Services; Sialic Acids; Simplexvirus; Structure; System; Telephone; United States National Institutes of Health; Work; interest; medical schools; nanobiotechnology; oncology; particle; trafficking; transmission process

DESCRIPTION (provided by applicant): A diverse group of 9 major and 8 minor users who are well funded NIH investigators, within the Johns Hopkins School of Arts & Sciences, Whiting School of Engineering, and the JHMI School of Medicine, request funds to purchase an FEI Tecnai F20 TWIN 200kV transmission electron microscope (TEM) with a Falcon II direct detection camera. This new state-of-the-art TEM will be housed in the Hopkins Integrated Imaging Center (www.jhu.edu/IIC) that is the only TEM facility on the Hopkins' Homewood campus; and is well utilized by the Hopkins-wide research community. It will replace a 30 year old Philips EM 420 for which service and day-to-day reliability has become critically dubious (see letter from John Fike, East Regional Service Manager, FEI in Appendix A); and perfectly complement an existing Tecnai 12. If funded, the Tecnai F20 will become the primary TEM platform for cryoelectron microscopy and low dose applications at Hopkins. Its 200kV capability, FEG, and Falcon II direct detection camera will add upgraded capabilities in cryo and molecular TEM, that are not currently present, astonishingly, anywhere at Hopkins; and it will free up our existing T12 for use in more conventional, routine TEM applications. Our investigators, all well-funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: HIV structure and function, HSV assembly and maturation; DNA regulation and chromosome suprastructure, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; sialic acid display and cell adhesion; and mechanisms of endocytosis. The new Tecnai F20 will ensure that our diverse group of investigators have continued and uninterrupted access to a TEM optimized for many disparate applications; as well as allowing for future growth in more exotic structural applications of cryoTEM and single particle analysis. The Tecnai F20 will be conveniently located, administered, and maintained, in the Biology Department's soon to be renovated Integrated Imaging Center (IIC), a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >150 laboratories and >400 users. The IIC is a Hopkins' Homewood campus core microscopy resource associated with the Institute for NanoBioTechnology (INBT); and the newly established Engineering in Oncology Center. It is jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering; and is utilized regularly by multiple schools/departments The new system will be incorporated into the IIC's existing recharge system to ensure recovery of funds for supplies and maintenance; and it will be made freely available to all interested users Hopkins-wide through our convenient IIC web-scheduler and smart phone app.

描述（由申请人提供）：在约翰霍普金斯大学艺术与科学学院，怀廷工程学院和JHMI医学院内，由9名主要用户和8名次要用户组成的不同群体，他们是资金充足的NIH研究人员，要求资金购买FEI Tecnai F20 TWIN 200kV透射电子显微镜（TEM）与Falcon II直接检测相机。这个新的最先进的TEM将被安置在霍普金斯综合成像中心（www.jhu.edu/IIC），这是霍普金斯大学霍姆伍德校区唯一的TEM设施；并且被整个霍普金斯大学的研究团体充分利用。它将取代已有30年历史的飞利浦em420，该设备的服务和日常可靠性已经变得非常可疑（参见附录a中FEI东部区域服务经理John Fike的来信）；完美地补充了现有的Tecnai 12。如果获得资助，Tecnai F20将成为霍普金斯大学低温电子显微镜和低剂量应用的主要TEM平台。它的200kV能力、FEG和Falcon II直接探测相机将增加低温和分子TEM的升级能力，令人惊讶的是，这些目前在霍普金斯大学的任何地方都不存在；它将释放我们现有的T12，用于更常规的TEM应用。我们的研究人员都是由美国国立卫生研究院资助的，他们研究一系列基本的细胞生物学、生物化学和生物分子相关问题，包括但不限于：HIV的结构和功能、HSV的组装和成熟；DNA调控与染色体上层结构、膜组织和动力学；内体/溶酶体动力学;膜贩运;聚合基因载体的贩运；唾液酸显示与细胞粘附；以及内吞作用的机制。新的Tecnai F20将确保我们不同的研究小组能够持续不间断地访问针对许多不同应用进行优化的TEM；以及允许未来在更奇特的结构应用低温透射电镜和单粒子分析的增长。Tecnai F20将在生物学系即将翻新的综合成像中心（IIC）中方便地定位，管理和维护，这是霍姆伍德校园/霍普金斯大学范围内的显微镜资源，由150个实验室和400个用户组成的多个学校和部门定期使用。IIC是霍普金斯大学霍姆伍德校区的核心显微镜资源，与纳米生物技术研究所（INBT）有关；以及新成立的肿瘤工程中心。它是由克里格艺术与科学学院和怀廷工程学院联合支持的；，并由多个学校/部门定期使用。新系统会并入综合教育中心现有的充值系统，以确保回收供应及维修的资金；并将通过方便的IIC网络调度程序和智能手机应用程序免费提供给所有感兴趣的霍普金斯大学用户。